Anaeromassilibacillus sp. An250

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Anaeromassilibacillus; unclassified Anaeromassilibacillus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3281 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4EWC9|A0A1Y4EWC9_9FIRM ATP-dependent DNA helicase RecG OS=Anaeromassilibacillus sp. An250 OX=1965604 GN=recG PE=3 SV=1
MM1 pKa = 7.01KK2 pKa = 9.4TWKK5 pKa = 9.88KK6 pKa = 10.29CLAAVLSLVMMVSATACSGTDD27 pKa = 3.14KK28 pKa = 10.9SWAVKK33 pKa = 10.38SEE35 pKa = 3.83NLTVPIGAYY44 pKa = 9.45IYY46 pKa = 10.31NLYY49 pKa = 10.36SAYY52 pKa = 9.55QQAYY56 pKa = 9.81YY57 pKa = 9.52MVEE60 pKa = 4.33DD61 pKa = 3.56ATQPVLEE68 pKa = 4.44QTVEE72 pKa = 4.12DD73 pKa = 3.66QDD75 pKa = 3.49AATWMKK81 pKa = 11.02DD82 pKa = 3.09FALRR86 pKa = 11.84QTKK89 pKa = 10.25SILVIDD95 pKa = 4.45DD96 pKa = 3.4MMRR99 pKa = 11.84DD100 pKa = 3.36MGLSLTEE107 pKa = 5.49DD108 pKa = 3.77EE109 pKa = 4.82LQQVSDD115 pKa = 3.74QTDD118 pKa = 3.77SFWGSISTAMTEE130 pKa = 3.99YY131 pKa = 10.92GVAKK135 pKa = 10.48SSFNLAYY142 pKa = 10.24ADD144 pKa = 4.37YY145 pKa = 8.33YY146 pKa = 11.1TKK148 pKa = 9.89FTKK151 pKa = 10.46VFDD154 pKa = 3.59ATYY157 pKa = 10.35GPGGTEE163 pKa = 3.79EE164 pKa = 4.81VSDD167 pKa = 4.44EE168 pKa = 4.15DD169 pKa = 4.0VKK171 pKa = 11.28DD172 pKa = 3.98YY173 pKa = 11.67YY174 pKa = 9.93LTNYY178 pKa = 9.86KK179 pKa = 10.41AFSYY183 pKa = 11.21VMAPIIDD190 pKa = 4.06MTTGASLSDD199 pKa = 4.41DD200 pKa = 4.09EE201 pKa = 5.99LSQLDD206 pKa = 4.2TEE208 pKa = 4.75FKK210 pKa = 10.83GYY212 pKa = 10.9ASQINDD218 pKa = 3.09GSMTIQDD225 pKa = 3.53AADD228 pKa = 3.77AYY230 pKa = 9.68KK231 pKa = 10.68ASSGDD236 pKa = 3.42DD237 pKa = 3.33TVQVYY242 pKa = 9.45TDD244 pKa = 3.36AADD247 pKa = 4.08FSGEE251 pKa = 4.07TASYY255 pKa = 9.07PLEE258 pKa = 4.72FGTEE262 pKa = 4.06LDD264 pKa = 3.46TLEE267 pKa = 4.29NGKK270 pKa = 10.62ASVIDD275 pKa = 3.59VQGLYY280 pKa = 10.33KK281 pKa = 10.58VLLVKK286 pKa = 10.78DD287 pKa = 5.16DD288 pKa = 4.06INATADD294 pKa = 3.46EE295 pKa = 5.09RR296 pKa = 11.84ISDD299 pKa = 3.77EE300 pKa = 4.07TSRR303 pKa = 11.84TSILQTMKK311 pKa = 10.63QEE313 pKa = 3.93EE314 pKa = 4.47FQAKK318 pKa = 9.44IDD320 pKa = 4.26DD321 pKa = 4.49AADD324 pKa = 3.64SYY326 pKa = 11.76NATVNQSAIDD336 pKa = 3.81SYY338 pKa = 11.19QPDD341 pKa = 3.63MFVQEE346 pKa = 4.41TSSVAASTDD355 pKa = 3.43EE356 pKa = 4.22TSSQEE361 pKa = 4.06GTDD364 pKa = 3.6GSGEE368 pKa = 4.18EE369 pKa = 4.45TSSEE373 pKa = 4.05AAA375 pKa = 3.63

Molecular weight: 41.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4ENZ6|A0A1Y4ENZ6_9FIRM LacI family transcriptional regulator OS=Anaeromassilibacillus sp. An250 OX=1965604 GN=B5F54_12440 PE=4 SV=1
MM1 pKa = 7.49TSFTNGVEE9 pKa = 4.66LIDD12 pKa = 4.12PRR14 pKa = 11.84CLLGKK19 pKa = 9.78HH20 pKa = 5.59EE21 pKa = 4.59KK22 pKa = 9.83AAPAIGCRR30 pKa = 11.84PFAFLHH36 pKa = 6.43IGTICLEE43 pKa = 3.77VRR45 pKa = 11.84IRR47 pKa = 11.84SAALIFLARR56 pKa = 11.84HH57 pKa = 4.78KK58 pKa = 9.45TAILGATLLAGVVRR72 pKa = 11.84QLRR75 pKa = 11.84GTGCQPVHH83 pKa = 6.52SRR85 pKa = 11.84IVAVGWQVPTCGVGVDD101 pKa = 3.72RR102 pKa = 11.84SSSVLEE108 pKa = 3.98KK109 pKa = 10.02SWRR112 pKa = 11.84TRR114 pKa = 11.84RR115 pKa = 11.84PAPRR119 pKa = 11.84TMPGAAAIQLWRR131 pKa = 11.84GSTPLIYY138 pKa = 9.89QGHH141 pKa = 6.69LEE143 pKa = 4.35DD144 pKa = 4.79VFF146 pKa = 4.87

Molecular weight: 15.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3281

0

3281

1017378

27

2863

310.1

34.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.999 ± 0.05

1.554 ± 0.021

5.715 ± 0.045

7.257 ± 0.044

3.918 ± 0.031

7.335 ± 0.035

1.835 ± 0.02

6.001 ± 0.043

5.336 ± 0.042

9.349 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.725 ± 0.018

3.831 ± 0.035

4.063 ± 0.03

3.771 ± 0.025

5.248 ± 0.054

5.777 ± 0.034

5.574 ± 0.051

6.959 ± 0.039

1.026 ± 0.015

3.728 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski