Streptococcus phage IPP34

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5SBJ0|A0A1S5SBJ0_9CAUD Uncharacterized protein OS=Streptococcus phage IPP34 OX=1916173 GN=IPP34_00032 PE=4 SV=1
MM1 pKa = 7.34MEE3 pKa = 4.11EE4 pKa = 4.65LKK6 pKa = 10.91QKK8 pKa = 10.59VNEE11 pKa = 4.25VYY13 pKa = 10.57NWTVEE18 pKa = 4.18DD19 pKa = 5.08GKK21 pKa = 9.85PQPPKK26 pKa = 10.18QDD28 pKa = 3.05LPQAVKK34 pKa = 10.82DD35 pKa = 3.53RR36 pKa = 11.84ADD38 pKa = 3.92YY39 pKa = 9.42FWEE42 pKa = 4.0MAEE45 pKa = 5.33DD46 pKa = 3.88GMTFMGAMEE55 pKa = 5.67CIFADD60 pKa = 4.73EE61 pKa = 5.02KK62 pKa = 10.3PTDD65 pKa = 3.84YY66 pKa = 11.56DD67 pKa = 4.51LGATKK72 pKa = 10.55DD73 pKa = 3.52WLPKK77 pKa = 10.51SKK79 pKa = 10.64EE80 pKa = 3.52FDD82 pKa = 3.25DD83 pKa = 3.91WVGYY87 pKa = 8.92SPSMAQVVIAVYY99 pKa = 10.36LIYY102 pKa = 10.64RR103 pKa = 11.84GNN105 pKa = 3.24

Molecular weight: 12.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5SBH1|A0A1S5SBH1_9CAUD Uncharacterized protein OS=Streptococcus phage IPP34 OX=1916173 GN=IPP34_00012 PE=4 SV=1
MM1 pKa = 6.88MVVLANHH8 pKa = 6.91PNWQVYY14 pKa = 9.15PDD16 pKa = 4.7EE17 pKa = 4.04IAKK20 pKa = 10.37RR21 pKa = 11.84KK22 pKa = 9.24GVSRR26 pKa = 11.84DD27 pKa = 3.45TVDD30 pKa = 3.51SYY32 pKa = 11.69FKK34 pKa = 10.48ILEE37 pKa = 4.11KK38 pKa = 10.65NGYY41 pKa = 9.01LRR43 pKa = 11.84IVKK46 pKa = 10.05KK47 pKa = 11.01GMGRR51 pKa = 11.84GKK53 pKa = 9.88GVRR56 pKa = 11.84VFRR59 pKa = 11.84FFSDD63 pKa = 3.14VKK65 pKa = 10.69ISDD68 pKa = 3.67FQFEE72 pKa = 4.23IMKK75 pKa = 10.12QRR77 pKa = 11.84LNEE80 pKa = 4.54SISKK84 pKa = 10.66LSTGG88 pKa = 3.63

Molecular weight: 10.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

12198

32

1848

182.1

20.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.698 ± 0.579

0.582 ± 0.104

6.231 ± 0.295

8.288 ± 0.525

4.255 ± 0.279

6.075 ± 0.605

1.599 ± 0.207

6.673 ± 0.277

8.493 ± 0.424

8.337 ± 0.348

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.541 ± 0.191

5.386 ± 0.296

2.492 ± 0.177

4.206 ± 0.209

4.624 ± 0.314

6.173 ± 0.271

6.099 ± 0.282

6.214 ± 0.265

1.303 ± 0.162

3.73 ± 0.319
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski