Erwinia phage vB_EamM_Y3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Sasquatchvirus; Erwinia virus Y3

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 333 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4IBF5|A0A2H4IBF5_9CAUD Uncharacterized protein OS=Erwinia phage vB_EamM_Y3 OX=1983553 GN=Y3_224 PE=4 SV=1
MM1 pKa = 6.52TTYY4 pKa = 10.7TSIYY8 pKa = 9.13EE9 pKa = 3.76IAEE12 pKa = 4.69AIDD15 pKa = 3.81GGLNSEE21 pKa = 4.68EE22 pKa = 4.03VAQALNITASAQEE35 pKa = 3.9ILVCGLQEE43 pKa = 4.27DD44 pKa = 4.52FGIYY48 pKa = 10.09VPLINDD54 pKa = 3.44QNYY57 pKa = 10.49LIADD61 pKa = 3.73TDD63 pKa = 4.08DD64 pKa = 4.48LFSEE68 pKa = 4.36HH69 pKa = 6.87LKK71 pKa = 10.63GQGAFTVCRR80 pKa = 11.84IFTPEE85 pKa = 3.85NEE87 pKa = 4.32MFDD90 pKa = 3.65QEE92 pKa = 4.37EE93 pKa = 4.76VEE95 pKa = 4.52VEE97 pKa = 3.85QLGEE101 pKa = 4.08FNDD104 pKa = 4.1FEE106 pKa = 4.37TALAAFVSAINTAANLKK123 pKa = 10.74AEE125 pKa = 4.54LEE127 pKa = 4.15AFAARR132 pKa = 11.84INQLLPDD139 pKa = 4.32DD140 pKa = 4.55RR141 pKa = 11.84SVVVFDD147 pKa = 6.1DD148 pKa = 4.25IEE150 pKa = 5.99DD151 pKa = 3.51EE152 pKa = 4.12AAYY155 pKa = 10.29MMIVKK160 pKa = 9.64VEE162 pKa = 4.06NGNVVEE168 pKa = 5.13DD169 pKa = 3.22IGYY172 pKa = 9.9SISHH176 pKa = 6.26SLSGTDD182 pKa = 4.49CSYY185 pKa = 12.03ADD187 pKa = 3.8AGDD190 pKa = 3.81YY191 pKa = 7.68PTIEE195 pKa = 4.09LGKK198 pKa = 8.92TDD200 pKa = 4.21EE201 pKa = 4.25EE202 pKa = 4.62VAANIAALTFF212 pKa = 4.14

Molecular weight: 23.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4IAX9|A0A2H4IAX9_9CAUD Oxygenase OS=Erwinia phage vB_EamM_Y3 OX=1983553 GN=Y3_020 PE=4 SV=1
MM1 pKa = 7.6DD2 pKa = 5.2HH3 pKa = 7.38KK4 pKa = 11.19DD5 pKa = 3.29FVIAVFVCCGLIAAVTVNVFAVRR28 pKa = 11.84SEE30 pKa = 4.01RR31 pKa = 11.84KK32 pKa = 9.54ARR34 pKa = 11.84ASYY37 pKa = 8.92WATPTIAVVQRR48 pKa = 11.84RR49 pKa = 11.84YY50 pKa = 10.19RR51 pKa = 11.84IARR54 pKa = 11.84RR55 pKa = 11.84VSLATIVLAIVIMAFAFGAHH75 pKa = 5.94AA76 pKa = 4.79

Molecular weight: 8.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

333

0

333

82035

39

1951

246.4

27.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.413 ± 0.194

1.096 ± 0.055

6.798 ± 0.164

5.858 ± 0.164

3.909 ± 0.087

6.289 ± 0.138

1.953 ± 0.079

5.594 ± 0.098

6.196 ± 0.255

8.343 ± 0.127

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.767 ± 0.078

4.481 ± 0.089

4.257 ± 0.077

3.803 ± 0.08

5.145 ± 0.106

6.913 ± 0.122

6.308 ± 0.17

7.26 ± 0.128

1.113 ± 0.05

3.502 ± 0.098
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski