Aeromonas phage SD04

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Queuovirinae; Seuratvirus; unclassified Seuratvirus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S7N8Y1|A0A3S7N8Y1_9CAUD Uncharacterized protein OS=Aeromonas phage SD04 OX=2201378 PE=4 SV=1
MM1 pKa = 7.36RR2 pKa = 11.84VEE4 pKa = 4.48EE5 pKa = 4.01IVEE8 pKa = 4.13GKK10 pKa = 9.87EE11 pKa = 3.61YY12 pKa = 10.96RR13 pKa = 11.84LLTDD17 pKa = 3.43IGGVGYY23 pKa = 8.56TPDD26 pKa = 3.97DD27 pKa = 3.76AACFNVLPQGAIVTVNKK44 pKa = 9.97VVTDD48 pKa = 4.39LYY50 pKa = 10.96SVHH53 pKa = 7.46DD54 pKa = 4.51GRR56 pKa = 11.84WPHH59 pKa = 5.98AVIVTFGKK67 pKa = 10.28DD68 pKa = 3.12GYY70 pKa = 11.32VDD72 pKa = 4.92LDD74 pKa = 4.3GEE76 pKa = 4.46DD77 pKa = 3.39GAYY80 pKa = 9.43EE81 pKa = 3.89VAYY84 pKa = 9.31TGLVSPNFLEE94 pKa = 4.59EE95 pKa = 4.0VV96 pKa = 3.43

Molecular weight: 10.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S7N8U2|A0A3S7N8U2_9CAUD Uncharacterized protein OS=Aeromonas phage SD04 OX=2201378 PE=4 SV=1
MM1 pKa = 7.54GLHH4 pKa = 6.14LQPYY8 pKa = 7.93EE9 pKa = 4.38CEE11 pKa = 3.74ILRR14 pKa = 11.84QLWLEE19 pKa = 4.0KK20 pKa = 10.13RR21 pKa = 11.84VSEE24 pKa = 4.59AEE26 pKa = 4.04QNAKK30 pKa = 10.53LMEE33 pKa = 4.3SQMQNQNKK41 pKa = 8.83GRR43 pKa = 11.84RR44 pKa = 11.84KK45 pKa = 9.48RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 3.21

Molecular weight: 5.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

14352

47

1017

214.2

23.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.727 ± 0.72

1.233 ± 0.159

5.665 ± 0.246

6.327 ± 0.295

3.477 ± 0.257

7.483 ± 0.284

1.672 ± 0.177

5.727 ± 0.193

6.166 ± 0.304

7.483 ± 0.292

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.961 ± 0.172

5.086 ± 0.238

4.25 ± 0.329

4.759 ± 0.373

4.738 ± 0.216

5.365 ± 0.26

5.874 ± 0.27

7.086 ± 0.296

1.568 ± 0.13

3.351 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski