Paenibacillus protaetiae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3615 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P6EW82|A0A4P6EW82_9BACL Uncharacterized protein OS=Paenibacillus protaetiae OX=2509456 GN=ET464_11615 PE=4 SV=1
MM1 pKa = 6.91YY2 pKa = 9.48CVCKK6 pKa = 10.55GHH8 pKa = 6.79VEE10 pKa = 3.82LAIDD14 pKa = 3.54KK15 pKa = 10.59FVDD18 pKa = 3.68EE19 pKa = 5.73FEE21 pKa = 4.75DD22 pKa = 4.43APDD25 pKa = 3.54IVDD28 pKa = 3.86LNKK31 pKa = 10.51TEE33 pKa = 4.09FADD36 pKa = 3.59WDD38 pKa = 3.87PPRR41 pKa = 11.84CCDD44 pKa = 3.98LCEE47 pKa = 4.0QNAEE51 pKa = 4.13FLVVV55 pKa = 3.51

Molecular weight: 6.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P6EZ03|A0A4P6EZ03_9BACL Carbohydrate ABC transporter permease OS=Paenibacillus protaetiae OX=2509456 GN=ET464_12125 PE=3 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.82PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.78VLAARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.23GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3615

0

3615

1155770

25

2793

319.7

35.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.306 ± 0.058

0.733 ± 0.012

5.158 ± 0.03

6.536 ± 0.04

3.944 ± 0.029

7.699 ± 0.039

2.111 ± 0.019

6.39 ± 0.04

5.142 ± 0.035

9.996 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.747 ± 0.025

3.611 ± 0.033

4.22 ± 0.028

4.005 ± 0.025

5.179 ± 0.039

6.229 ± 0.031

5.247 ± 0.039

7.066 ± 0.035

1.252 ± 0.018

3.427 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski