Lysobacter enzymogenes

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Lysobacter

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5514 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S2DG49|A0A0S2DG49_LYSEN Uncharacterized protein OS=Lysobacter enzymogenes OX=69 GN=GLE_2131 PE=4 SV=1
MM1 pKa = 7.37GAATDD6 pKa = 3.66IEE8 pKa = 4.58QINLSNPTATGDD20 pKa = 3.93DD21 pKa = 3.79ANGTDD26 pKa = 5.24DD27 pKa = 4.2EE28 pKa = 5.17DD29 pKa = 4.67AFASIPAIPLVAGGTYY45 pKa = 10.41NLSVPCAGNGAAVSGFIDD63 pKa = 4.15FNRR66 pKa = 11.84DD67 pKa = 2.15GDD69 pKa = 4.06FADD72 pKa = 4.12SGEE75 pKa = 4.13KK76 pKa = 10.07SAAATCNGSTAALTWTLPGAAGLNAGASFVRR107 pKa = 11.84LRR109 pKa = 11.84IGTQAAQVNVPTGLASDD126 pKa = 4.86GEE128 pKa = 4.5VEE130 pKa = 4.81DD131 pKa = 4.23YY132 pKa = 11.32AVTLTSPTLTLRR144 pKa = 11.84KK145 pKa = 8.75QWNGATTGDD154 pKa = 4.09DD155 pKa = 3.57AAVTASRR162 pKa = 11.84GGTVVGTLNSDD173 pKa = 3.18AGSANEE179 pKa = 5.0LDD181 pKa = 3.37TATAINVFPGEE192 pKa = 4.27TLTLAEE198 pKa = 4.22TLAANGGRR206 pKa = 11.84TYY208 pKa = 9.84TQSLACTGTSDD219 pKa = 5.1ANPNDD224 pKa = 3.52GLTIGAADD232 pKa = 3.86ANIVCTFTNAQAARR246 pKa = 11.84LTVIKK251 pKa = 10.75NVINDD256 pKa = 3.63NGGVATVADD265 pKa = 4.54FGIQVDD271 pKa = 4.65GVARR275 pKa = 11.84SFGANTGTAGNAVYY289 pKa = 9.7TSAAVAVNAGTRR301 pKa = 11.84ALTEE305 pKa = 4.41LNVAGYY311 pKa = 8.28TEE313 pKa = 4.57GTWACTGTGVTMGNAAFDD331 pKa = 3.99SGSVTLAAGADD342 pKa = 3.74ATCTITNDD350 pKa = 3.49DD351 pKa = 4.07TPASLTLRR359 pKa = 11.84KK360 pKa = 9.72AVVNDD365 pKa = 3.55NGGTAAQTAWTLNAAGPTPISGTHH389 pKa = 5.7GAAAVTNAAVGAGTYY404 pKa = 8.02TLSEE408 pKa = 4.23SGGPAGYY415 pKa = 7.42TASAYY420 pKa = 10.34SCSVDD425 pKa = 2.77GGAAVSGNSLTLANGQNAVCTITNDD450 pKa = 3.55DD451 pKa = 4.37APAQLTLQKK460 pKa = 10.89VVVNDD465 pKa = 3.39NGGAAVATAWTLNANGPTPISGTHH489 pKa = 5.92AAAAVTNATVGAGTYY504 pKa = 7.95TLSEE508 pKa = 4.23SGGPAGYY515 pKa = 7.42TASAYY520 pKa = 10.47SCSIDD525 pKa = 2.97GGAAVSGNSLTLANGQNAVCTITNDD550 pKa = 3.58DD551 pKa = 3.81APANLTLRR559 pKa = 11.84KK560 pKa = 7.83TVVNNNGGTAAATAWTLNAAGPTPISGTHH589 pKa = 6.13GSAAVTNAAVGAGTYY604 pKa = 7.9TLSEE608 pKa = 4.25TGPAGYY614 pKa = 9.41NASAWVCTNGVTVTGGNQITLANGATTDD642 pKa = 3.74CTITNDD648 pKa = 3.19DD649 pKa = 3.73RR650 pKa = 11.84QAVLSLTKK658 pKa = 10.3RR659 pKa = 11.84VINDD663 pKa = 3.22NGGTRR668 pKa = 11.84LASAWTLRR676 pKa = 11.84ATGPTAVSGTPPVLPRR692 pKa = 11.84PVNAGTYY699 pKa = 7.55TLSEE703 pKa = 4.29TGPGGYY709 pKa = 9.53AASAWTCTNGVTVTGGNQITLPPGGGTSCQITNDD743 pKa = 3.95DD744 pKa = 3.98RR745 pKa = 11.84PATLTLQKK753 pKa = 10.36IVVNDD758 pKa = 3.6NGGTAVATAWTLNAAGPTPISGAHH782 pKa = 5.88GAAAVTGAAVNAGTYY797 pKa = 10.42ALSEE801 pKa = 4.34SGGPADD807 pKa = 4.12YY808 pKa = 8.54TASAYY813 pKa = 10.55SCSIDD818 pKa = 2.97GGAAVSGDD826 pKa = 3.94SLTLANGQSAVCTVTNDD843 pKa = 4.29DD844 pKa = 4.39SNQADD849 pKa = 3.82LSITKK854 pKa = 10.49SNTYY858 pKa = 9.41TPADD862 pKa = 3.91PSDD865 pKa = 3.58QVGDD869 pKa = 3.82TVVAGTPTTYY879 pKa = 10.79TLVVTNNGPATAVGAVVRR897 pKa = 11.84DD898 pKa = 3.95VPQAGLDD905 pKa = 3.88CPASNPVACSGAACPSAAITIGDD928 pKa = 4.22LGSGITLGSLATGATATLSFTCAVQQQ954 pKa = 3.9

Molecular weight: 92.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S2DMV5|A0A0S2DMV5_LYSEN Aminopeptidase N OS=Lysobacter enzymogenes OX=69 GN=lta4h PE=3 SV=1
MM1 pKa = 7.13QRR3 pKa = 11.84RR4 pKa = 11.84SPHH7 pKa = 5.79GPAGTGGRR15 pKa = 11.84ARR17 pKa = 11.84PARR20 pKa = 11.84GAPRR24 pKa = 11.84MPRR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84GFRR33 pKa = 11.84ARR35 pKa = 11.84AAPARR40 pKa = 11.84AFARR44 pKa = 11.84FGTVFRR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84GAAPPPSS60 pKa = 3.51

Molecular weight: 6.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5514

0

5514

1754100

37

9289

318.1

34.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.471 ± 0.062

0.893 ± 0.012

5.916 ± 0.032

5.13 ± 0.036

3.276 ± 0.023

8.791 ± 0.05

2.065 ± 0.019

3.684 ± 0.026

2.592 ± 0.037

10.5 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.843 ± 0.018

2.46 ± 0.029

5.626 ± 0.032

3.764 ± 0.026

8.361 ± 0.048

5.222 ± 0.027

4.488 ± 0.056

6.805 ± 0.028

1.556 ± 0.016

2.452 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski