Paraburkholderia piptadeniae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9923 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N7SB61|A0A1N7SB61_9BURK Putative effector of murein hydrolase LrgA OS=Paraburkholderia piptadeniae OX=1701573 GN=BN2476_420067 PE=4 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84ISTSQYY8 pKa = 9.89FGMNVQTMDD17 pKa = 3.72DD18 pKa = 3.76QQVTLAQLYY27 pKa = 8.39QQLSSGVSLATPSDD41 pKa = 3.92NPVGAAQAVQLSMQGTTLSQYY62 pKa = 11.47ASNQNTALSSLQAEE76 pKa = 4.63DD77 pKa = 4.07SSLSSISSVLTAINTQIVHH96 pKa = 6.72AGDD99 pKa = 4.01GSLNDD104 pKa = 4.07ANRR107 pKa = 11.84STIATEE113 pKa = 4.05LEE115 pKa = 4.27GMRR118 pKa = 11.84SQLMGLANGTDD129 pKa = 3.17ASGNYY134 pKa = 10.22LYY136 pKa = 10.88GGYY139 pKa = 9.2QASTPPFSVGTSGAVQYY156 pKa = 11.19SGDD159 pKa = 3.52NGVQYY164 pKa = 11.05VQVTSSRR171 pKa = 11.84SIAVADD177 pKa = 3.41TGKK180 pKa = 10.69AVFLSSSQPGSTDD193 pKa = 2.87VTAGALSNSGTGTIGGVTVNNSTDD217 pKa = 3.11PTNNHH222 pKa = 6.44KK223 pKa = 9.38FTINFTSGTTFTVTDD238 pKa = 3.76VATSTTSAPQTYY250 pKa = 8.07TAGQPIVLGGGGQSVTISGAPNGGDD275 pKa = 3.14SFSVTPAGQTSDD287 pKa = 3.37VFANLDD293 pKa = 3.6TVIAALQTPVTGATGQANLTNALGTALTQLQNTLNNVTTIQTSVGGRR340 pKa = 11.84EE341 pKa = 3.87QEE343 pKa = 3.9IEE345 pKa = 3.92ALQTVTQSNSLQTQSNLADD364 pKa = 3.53LTQTNLTKK372 pKa = 10.68VISQYY377 pKa = 11.24TMTQSSLQAAQQGFSMIQKK396 pKa = 10.32LSLFDD401 pKa = 4.21YY402 pKa = 10.72IGNN405 pKa = 3.88

Molecular weight: 41.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N7RWK2|A0A1N7RWK2_9BURK Galactonate dehydratase OS=Paraburkholderia piptadeniae OX=1701573 GN=dgoD PE=4 SV=1
MM1 pKa = 8.06RR2 pKa = 11.84PRR4 pKa = 11.84LKK6 pKa = 10.76LLFWFRR12 pKa = 11.84RR13 pKa = 11.84MMIRR17 pKa = 11.84SYY19 pKa = 9.16PQPARR24 pKa = 11.84RR25 pKa = 11.84HH26 pKa = 4.4GRR28 pKa = 11.84EE29 pKa = 3.81RR30 pKa = 11.84RR31 pKa = 11.84PAGPPWIPPAQQARR45 pKa = 11.84ALPLQAVLSRR55 pKa = 11.84RR56 pKa = 11.84PTT58 pKa = 3.19

Molecular weight: 6.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9923

0

9923

2772491

20

3122

279.4

30.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.295 ± 0.037

1.078 ± 0.01

5.432 ± 0.022

5.131 ± 0.024

3.715 ± 0.017

8.037 ± 0.028

2.42 ± 0.013

4.797 ± 0.018

3.213 ± 0.022

9.906 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.476 ± 0.012

2.878 ± 0.02

5.03 ± 0.019

3.547 ± 0.014

7.175 ± 0.027

5.922 ± 0.024

5.477 ± 0.022

7.685 ± 0.02

1.371 ± 0.01

2.418 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski