Sauropus yellowing virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus; unclassified Polerovirus

Average proteome isoelectric point is 8.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7EAU5|A0A0A7EAU5_9LUTE Serine protease OS=Sauropus yellowing virus OX=1577997 PE=4 SV=1
MM1 pKa = 7.54AGYY4 pKa = 10.69YY5 pKa = 8.3LTTFLVLLFLFSHH18 pKa = 6.58SSSFPTYY25 pKa = 10.31DD26 pKa = 3.12GRR28 pKa = 11.84VEE30 pKa = 4.56GNFTSTFPMLPPLSNGDD47 pKa = 3.59GGSDD51 pKa = 3.17ISPVSVLMEE60 pKa = 3.94TLYY63 pKa = 11.27VSISDD68 pKa = 4.28EE69 pKa = 4.28PPVAPLTNYY78 pKa = 9.99TYY80 pKa = 11.16GALLGAVSSKK90 pKa = 10.59FVFDD94 pKa = 4.96TIALCNEE101 pKa = 3.93AQEE104 pKa = 4.03IWVTLFRR111 pKa = 11.84SFCDD115 pKa = 3.2NTVNGLKK122 pKa = 9.49WAIEE126 pKa = 3.88KK127 pKa = 10.76LILSLVSIWSWLVYY141 pKa = 9.56QAILCILSCAVNYY154 pKa = 10.71APVLITLVLCGVCTRR169 pKa = 11.84YY170 pKa = 10.48LFLAVIWMYY179 pKa = 10.72GIQVTCLLSFLRR191 pKa = 11.84IISKK195 pKa = 8.07VLRR198 pKa = 11.84FRR200 pKa = 11.84RR201 pKa = 11.84PSGFKK206 pKa = 10.17NEE208 pKa = 3.9KK209 pKa = 9.32SVEE212 pKa = 4.1GFTEE216 pKa = 3.91ITFEE220 pKa = 3.79QSPPGKK226 pKa = 9.95SVLLLEE232 pKa = 4.29EE233 pKa = 4.06RR234 pKa = 11.84RR235 pKa = 11.84NGEE238 pKa = 3.95KK239 pKa = 10.24RR240 pKa = 11.84HH241 pKa = 5.53VGYY244 pKa = 11.09AFAGKK249 pKa = 10.01LYY251 pKa = 10.01NGEE254 pKa = 4.04VGLFTVAHH262 pKa = 6.07NLEE265 pKa = 4.49SCDD268 pKa = 3.85NLWVLSEE275 pKa = 4.1RR276 pKa = 11.84TKK278 pKa = 11.4NSLKK282 pKa = 10.58LKK284 pKa = 9.36QFQPIVLSKK293 pKa = 10.9SADD296 pKa = 3.51LAILRR301 pKa = 11.84GPVNWEE307 pKa = 3.62ALLGCKK313 pKa = 10.12GGIYY317 pKa = 7.07TTSNRR322 pKa = 11.84LAVSGCTWFDD332 pKa = 3.48YY333 pKa = 9.91TEE335 pKa = 3.89SWRR338 pKa = 11.84SHH340 pKa = 4.4SAKK343 pKa = 10.48VVGTDD348 pKa = 3.13GDD350 pKa = 4.1LVRR353 pKa = 11.84VLSNTRR359 pKa = 11.84PGHH362 pKa = 5.96SGTPYY367 pKa = 10.61FNGKK371 pKa = 9.12SCLGLHH377 pKa = 6.08KK378 pKa = 10.29GYY380 pKa = 10.83RR381 pKa = 11.84LAGDD385 pKa = 4.23NNNCMVPIPPIPGYY399 pKa = 6.53TTPDD403 pKa = 3.37YY404 pKa = 10.97KK405 pKa = 11.05FEE407 pKa = 4.16TSAPQGLLFDD417 pKa = 4.52SKK419 pKa = 10.07TIARR423 pKa = 11.84LEE425 pKa = 4.07KK426 pKa = 9.88QAMEE430 pKa = 4.4MVRR433 pKa = 11.84HH434 pKa = 6.24SGGVWDD440 pKa = 5.15FDD442 pKa = 3.94DD443 pKa = 4.46AFAVVSSDD451 pKa = 3.59YY452 pKa = 11.3VGEE455 pKa = 4.32TTLPKK460 pKa = 10.15PDD462 pKa = 3.29VDD464 pKa = 4.09EE465 pKa = 4.6SNKK468 pKa = 9.63IPKK471 pKa = 9.51DD472 pKa = 3.42HH473 pKa = 7.09QSFLRR478 pKa = 11.84DD479 pKa = 3.41YY480 pKa = 9.67GTVLTKK486 pKa = 10.72HH487 pKa = 6.31FGQYY491 pKa = 9.49QSPDD495 pKa = 3.26SAVDD499 pKa = 3.96LNLLFPGVKK508 pKa = 9.55PEE510 pKa = 3.74QAQQKK515 pKa = 8.9KK516 pKa = 9.92SSGNRR521 pKa = 11.84VSRR524 pKa = 11.84AGGKK528 pKa = 7.75NTGIRR533 pKa = 11.84RR534 pKa = 11.84TIHH537 pKa = 4.97PFEE540 pKa = 4.33RR541 pKa = 11.84KK542 pKa = 8.98RR543 pKa = 11.84RR544 pKa = 11.84YY545 pKa = 7.95EE546 pKa = 3.62ANRR549 pKa = 11.84AHH551 pKa = 6.89GCAYY555 pKa = 9.51WADD558 pKa = 3.55QLRR561 pKa = 11.84EE562 pKa = 3.81NRR564 pKa = 11.84RR565 pKa = 11.84TGSRR569 pKa = 11.84QNSEE573 pKa = 4.32FVDD576 pKa = 3.71EE577 pKa = 4.54TTQDD581 pKa = 3.27TRR583 pKa = 11.84SSWRR587 pKa = 11.84KK588 pKa = 8.93EE589 pKa = 3.29EE590 pKa = 3.67ARR592 pKa = 11.84NIRR595 pKa = 11.84EE596 pKa = 4.24HH597 pKa = 6.07FASRR601 pKa = 11.84YY602 pKa = 5.59QWNIPSPTPEE612 pKa = 3.7VEE614 pKa = 4.22GFQACGTFAYY624 pKa = 10.03RR625 pKa = 11.84YY626 pKa = 9.07HH627 pKa = 7.14CKK629 pKa = 9.79QLQEE633 pKa = 4.28SDD635 pKa = 2.62WGRR638 pKa = 11.84FLTGQYY644 pKa = 8.53PALGEE649 pKa = 4.08EE650 pKa = 4.4TRR652 pKa = 11.84GFGWPEE658 pKa = 3.26KK659 pKa = 10.46GAAAEE664 pKa = 4.1LKK666 pKa = 10.29SLKK669 pKa = 10.19LQASRR674 pKa = 11.84WRR676 pKa = 11.84EE677 pKa = 3.44RR678 pKa = 11.84ANRR681 pKa = 11.84AEE683 pKa = 4.23IPTGSARR690 pKa = 11.84QNVIEE695 pKa = 4.13QTVRR699 pKa = 11.84AYY701 pKa = 10.78SAVANTDD708 pKa = 2.95TPLCVRR714 pKa = 11.84SGQLVWSDD722 pKa = 3.98FIEE725 pKa = 4.49DD726 pKa = 4.22FKK728 pKa = 11.44QAVSTLEE735 pKa = 3.7LDD737 pKa = 3.63AGVGIPYY744 pKa = 9.97VEE746 pKa = 4.57FGKK749 pKa = 8.53PTHH752 pKa = 6.66RR753 pKa = 11.84GWIEE757 pKa = 3.79DD758 pKa = 3.81SYY760 pKa = 11.18LAPILAQLTFDD771 pKa = 4.5RR772 pKa = 11.84LQKK775 pKa = 9.96MSEE778 pKa = 4.07VNLAEE783 pKa = 4.14LTSAEE788 pKa = 4.32SLVQLGLCDD797 pKa = 5.01PIRR800 pKa = 11.84TFVKK804 pKa = 10.77GEE806 pKa = 3.68PHH808 pKa = 6.04KK809 pKa = 10.35QAKK812 pKa = 9.74LDD814 pKa = 3.49EE815 pKa = 4.35GRR817 pKa = 11.84YY818 pKa = 8.74RR819 pKa = 11.84LIMSVSLVDD828 pKa = 3.43QMVARR833 pKa = 11.84VLFQQQNKK841 pKa = 9.77RR842 pKa = 11.84EE843 pKa = 3.99IALWRR848 pKa = 11.84AIPSKK853 pKa = 10.43PGFGVSSDD861 pKa = 3.5EE862 pKa = 3.97QVAEE866 pKa = 4.95FISLLAQSVGKK877 pKa = 8.38TPIEE881 pKa = 4.22TVGEE885 pKa = 4.05WKK887 pKa = 10.37KK888 pKa = 11.18YY889 pKa = 10.23IIPTDD894 pKa = 3.4CSGFDD899 pKa = 3.21WSVAEE904 pKa = 4.61WMLDD908 pKa = 2.98DD909 pKa = 5.76DD910 pKa = 4.15MEE912 pKa = 4.33VRR914 pKa = 11.84NRR916 pKa = 11.84LTANNNGLLRR926 pKa = 11.84KK927 pKa = 9.55LRR929 pKa = 11.84AVWLQCIKK937 pKa = 10.82QSVLCLSDD945 pKa = 3.79GSLLAQRR952 pKa = 11.84IPGIQKK958 pKa = 9.39SGSYY962 pKa = 8.27NTSSSNSRR970 pKa = 11.84IRR972 pKa = 11.84VMCALHH978 pKa = 6.98CGAPWAIAMGDD989 pKa = 3.65DD990 pKa = 3.51ALEE993 pKa = 4.51GVGSDD998 pKa = 2.94LALYY1002 pKa = 9.79HH1003 pKa = 6.88KK1004 pKa = 10.6LGLKK1008 pKa = 10.24VEE1010 pKa = 4.27VSPKK1014 pKa = 10.82LEE1016 pKa = 4.26FCSHH1020 pKa = 6.37LFEE1023 pKa = 6.76SEE1025 pKa = 4.09TLALPVNINKK1035 pKa = 8.29MLWKK1039 pKa = 10.33LIYY1042 pKa = 10.0GYY1044 pKa = 11.02NPASGNLEE1052 pKa = 4.13VISNYY1057 pKa = 10.05LAACASVMHH1066 pKa = 6.45EE1067 pKa = 4.3MRR1069 pKa = 11.84HH1070 pKa = 5.89LPDD1073 pKa = 3.79KK1074 pKa = 11.16VDD1076 pKa = 3.86IIKK1079 pKa = 10.15RR1080 pKa = 11.84WLVAPIQPQNN1090 pKa = 3.18

Molecular weight: 122.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7E9K4|A0A0A7E9K4_9LUTE Readthrough protein OS=Sauropus yellowing virus OX=1577997 PE=3 SV=1
MM1 pKa = 6.62NTAGRR6 pKa = 11.84KK7 pKa = 7.97GRR9 pKa = 11.84RR10 pKa = 11.84GNGKK14 pKa = 9.38RR15 pKa = 11.84RR16 pKa = 11.84FNNVTRR22 pKa = 11.84QQRR25 pKa = 11.84TIQPVVVVTPNGQPRR40 pKa = 11.84GRR42 pKa = 11.84NRR44 pKa = 11.84GRR46 pKa = 11.84RR47 pKa = 11.84NRR49 pKa = 11.84LRR51 pKa = 11.84NRR53 pKa = 11.84GNRR56 pKa = 11.84GRR58 pKa = 11.84TARR61 pKa = 11.84GSVQSEE67 pKa = 4.02TFVFNKK73 pKa = 10.26DD74 pKa = 3.39DD75 pKa = 4.31LKK77 pKa = 11.22GSSHH81 pKa = 5.45GTIKK85 pKa = 10.75FGPNLSEE92 pKa = 4.39SVALSAGVLKK102 pKa = 10.51AYY104 pKa = 10.31HH105 pKa = 6.65EE106 pKa = 4.46YY107 pKa = 10.43KK108 pKa = 10.15IVMVNIRR115 pKa = 11.84FVSEE119 pKa = 4.22SSSTAQGSIAYY130 pKa = 9.28EE131 pKa = 3.94LDD133 pKa = 3.38PHH135 pKa = 7.0CKK137 pKa = 9.85LDD139 pKa = 3.78ALKK142 pKa = 9.62STLRR146 pKa = 11.84KK147 pKa = 9.65FPITKK152 pKa = 10.07GGQATFRR159 pKa = 11.84ASEE162 pKa = 3.93INGEE166 pKa = 4.16KK167 pKa = 9.35WHH169 pKa = 6.92DD170 pKa = 3.53TTVDD174 pKa = 3.49QFRR177 pKa = 11.84LLYY180 pKa = 9.71KK181 pKa = 10.9GNGAASEE188 pKa = 4.24TAGFFQIRR196 pKa = 11.84FTVQLHH202 pKa = 5.06NPKK205 pKa = 10.54

Molecular weight: 22.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3036

161

1090

506.0

56.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.192 ± 0.432

1.68 ± 0.257

4.545 ± 0.502

5.567 ± 0.346

4.348 ± 0.462

7.444 ± 0.46

1.91 ± 0.276

4.578 ± 0.312

5.27 ± 0.492

9.519 ± 0.702

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.548 ± 0.258

4.348 ± 0.473

5.369 ± 0.528

3.722 ± 0.419

7.016 ± 0.987

8.959 ± 0.597

6.061 ± 0.465

6.719 ± 0.353

1.877 ± 0.291

3.294 ± 0.29
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski