Semliki forest virus (SFV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Togaviridae; Alphavirus

Average proteome isoelectric point is 7.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

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* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>sp|P08411|POLN_SFV Polyprotein P1234 OS=Semliki forest virus OX=11033 PE=1 SV=2
MM1 pKa = 7.23AAKK4 pKa = 10.06VHH6 pKa = 5.91VDD8 pKa = 3.51IEE10 pKa = 4.4ADD12 pKa = 3.65SPFIKK17 pKa = 10.3SLQKK21 pKa = 10.5AFPSFEE27 pKa = 4.18VEE29 pKa = 4.31SLQVTPNDD37 pKa = 3.7HH38 pKa = 6.54ANARR42 pKa = 11.84AFSHH46 pKa = 7.06LATKK50 pKa = 10.64LIEE53 pKa = 3.98QEE55 pKa = 3.91TDD57 pKa = 2.69KK58 pKa = 11.15DD59 pKa = 3.98TLILDD64 pKa = 3.86IGSAPSRR71 pKa = 11.84RR72 pKa = 11.84MMSTHH77 pKa = 7.32KK78 pKa = 9.23YY79 pKa = 9.86HH80 pKa = 6.77CVCPMRR86 pKa = 11.84SAEE89 pKa = 4.02DD90 pKa = 3.77PEE92 pKa = 5.43RR93 pKa = 11.84LVCYY97 pKa = 10.05AKK99 pKa = 10.67KK100 pKa = 10.22LAAASGKK107 pKa = 10.13VLDD110 pKa = 4.57RR111 pKa = 11.84EE112 pKa = 4.24IAGKK116 pKa = 8.37ITDD119 pKa = 4.55LQTVMATPDD128 pKa = 3.35AEE130 pKa = 4.61SPTFCLHH137 pKa = 6.47TDD139 pKa = 3.67VTCRR143 pKa = 11.84TAAEE147 pKa = 4.04VAVYY151 pKa = 9.92QDD153 pKa = 3.71VYY155 pKa = 11.3AVHH158 pKa = 7.13APTSLYY164 pKa = 8.74HH165 pKa = 5.53QAMKK169 pKa = 10.56GVRR172 pKa = 11.84TAYY175 pKa = 9.57WIGFDD180 pKa = 3.41TTPFMFDD187 pKa = 3.6ALAGAYY193 pKa = 7.07PTYY196 pKa = 9.32ATNWADD202 pKa = 3.71EE203 pKa = 4.23QVLQARR209 pKa = 11.84NIGLCAASLTEE220 pKa = 3.92GRR222 pKa = 11.84LGKK225 pKa = 10.6LSILRR230 pKa = 11.84KK231 pKa = 9.59KK232 pKa = 8.34QLKK235 pKa = 9.77PCDD238 pKa = 3.22TVMFSVGSTLYY249 pKa = 10.03TEE251 pKa = 4.25SRR253 pKa = 11.84KK254 pKa = 9.79LLRR257 pKa = 11.84SWHH260 pKa = 6.11LPSVFHH266 pKa = 6.91LKK268 pKa = 10.36GKK270 pKa = 10.29QSFTCRR276 pKa = 11.84CDD278 pKa = 3.56TIVSCEE284 pKa = 4.06GYY286 pKa = 9.24VVKK289 pKa = 10.51KK290 pKa = 9.56ITMCPGLYY298 pKa = 10.36GKK300 pKa = 7.94TVGYY304 pKa = 10.56AVTYY308 pKa = 9.17HH309 pKa = 7.04AEE311 pKa = 3.99GFLVCKK317 pKa = 8.49TTDD320 pKa = 3.15TVKK323 pKa = 10.8GEE325 pKa = 3.97RR326 pKa = 11.84VSFPVCTYY334 pKa = 10.42VPSTICDD341 pKa = 3.17QMTGILATDD350 pKa = 3.81VTPEE354 pKa = 4.08DD355 pKa = 3.7AQKK358 pKa = 11.43LLVGLNQRR366 pKa = 11.84IVVNGRR372 pKa = 11.84TQRR375 pKa = 11.84NTNTMKK381 pKa = 10.77NYY383 pKa = 9.87LLPIVAVAFSKK394 pKa = 10.07WARR397 pKa = 11.84EE398 pKa = 3.97YY399 pKa = 11.11KK400 pKa = 10.46ADD402 pKa = 5.03LDD404 pKa = 4.13DD405 pKa = 4.76EE406 pKa = 4.82KK407 pKa = 11.17PLGVRR412 pKa = 11.84EE413 pKa = 4.24RR414 pKa = 11.84SLTCCCLWAFKK425 pKa = 8.5TRR427 pKa = 11.84KK428 pKa = 7.16MHH430 pKa = 5.9TMYY433 pKa = 10.75KK434 pKa = 10.3KK435 pKa = 10.23PDD437 pKa = 3.19TQTIVKK443 pKa = 9.62VPSEE447 pKa = 4.24FNSFVIPSLWSTGLAIPVRR466 pKa = 11.84SRR468 pKa = 11.84IKK470 pKa = 9.99MLLAKK475 pKa = 8.89KK476 pKa = 8.15TKK478 pKa = 10.21RR479 pKa = 11.84EE480 pKa = 4.4LIPVLDD486 pKa = 4.13ASSARR491 pKa = 11.84DD492 pKa = 3.34AEE494 pKa = 4.29QEE496 pKa = 3.96EE497 pKa = 4.73KK498 pKa = 10.81EE499 pKa = 4.09RR500 pKa = 11.84LEE502 pKa = 5.02AEE504 pKa = 4.47LTRR507 pKa = 11.84EE508 pKa = 4.15ALPPLVPIAPAEE520 pKa = 4.2TGVVDD525 pKa = 3.64VDD527 pKa = 3.95VEE529 pKa = 4.15EE530 pKa = 4.93LEE532 pKa = 4.22YY533 pKa = 10.77HH534 pKa = 6.51AGAGVVEE541 pKa = 4.79TPRR544 pKa = 11.84SALKK548 pKa = 9.32VTAQPNDD555 pKa = 3.56VLLGNYY561 pKa = 8.74VVLSPQTVLKK571 pKa = 10.3SSKK574 pKa = 9.66LAPVHH579 pKa = 6.33PLAEE583 pKa = 3.93QVKK586 pKa = 9.55IITHH590 pKa = 5.83NGRR593 pKa = 11.84AGRR596 pKa = 11.84YY597 pKa = 7.55QVDD600 pKa = 4.3GYY602 pKa = 10.79DD603 pKa = 3.68GRR605 pKa = 11.84VLLPCGSAIPVPEE618 pKa = 4.17FQALSEE624 pKa = 4.4SATMVYY630 pKa = 10.35NEE632 pKa = 4.29RR633 pKa = 11.84EE634 pKa = 3.94FVNRR638 pKa = 11.84KK639 pKa = 8.6LYY641 pKa = 10.49HH642 pKa = 6.12IAVHH646 pKa = 6.41GPSLNTDD653 pKa = 3.18EE654 pKa = 4.84EE655 pKa = 4.67NYY657 pKa = 10.34EE658 pKa = 3.99KK659 pKa = 10.92VRR661 pKa = 11.84AEE663 pKa = 4.04RR664 pKa = 11.84TDD666 pKa = 3.05AEE668 pKa = 4.68YY669 pKa = 11.44VFDD672 pKa = 4.53VDD674 pKa = 5.45KK675 pKa = 10.84KK676 pKa = 9.22CCVKK680 pKa = 10.6RR681 pKa = 11.84EE682 pKa = 4.03EE683 pKa = 4.17ASGLVLVGEE692 pKa = 4.44LTNPPFHH699 pKa = 6.66EE700 pKa = 4.38FAYY703 pKa = 9.79EE704 pKa = 3.92GLKK707 pKa = 9.91IRR709 pKa = 11.84PSAPYY714 pKa = 7.96KK715 pKa = 7.34TTVVGVFGVPGSGKK729 pKa = 9.82SAIIKK734 pKa = 10.36SLVTKK739 pKa = 10.42HH740 pKa = 6.94DD741 pKa = 4.03LVTSGKK747 pKa = 10.06KK748 pKa = 9.65EE749 pKa = 3.77NCQEE753 pKa = 3.73IVNDD757 pKa = 3.88VKK759 pKa = 10.98KK760 pKa = 10.29HH761 pKa = 5.86RR762 pKa = 11.84GLDD765 pKa = 3.49IQAKK769 pKa = 7.54TVDD772 pKa = 4.04SILLNGCRR780 pKa = 11.84RR781 pKa = 11.84AVDD784 pKa = 3.14ILYY787 pKa = 10.27VDD789 pKa = 3.87EE790 pKa = 6.07AFACHH795 pKa = 6.76SGTLLALIALVKK807 pKa = 10.09PRR809 pKa = 11.84SKK811 pKa = 10.93VVLCGDD817 pKa = 4.33PKK819 pKa = 10.69QCGFFNMMQLKK830 pKa = 10.72VNFNHH835 pKa = 6.98NICTEE840 pKa = 4.09VCHH843 pKa = 6.6KK844 pKa = 10.51SISRR848 pKa = 11.84RR849 pKa = 11.84CTRR852 pKa = 11.84PVTAIVSTLHH862 pKa = 6.15YY863 pKa = 8.59GGKK866 pKa = 8.54MRR868 pKa = 11.84TTNPCNKK875 pKa = 9.52PIIIDD880 pKa = 3.46TTGQTKK886 pKa = 9.88PKK888 pKa = 9.8PGDD891 pKa = 3.18IVLTCFRR898 pKa = 11.84GWVKK902 pKa = 10.19QLQLDD907 pKa = 4.14YY908 pKa = 10.75RR909 pKa = 11.84GHH911 pKa = 5.99EE912 pKa = 4.46VMTAAASQGLTRR924 pKa = 11.84KK925 pKa = 9.35GVYY928 pKa = 9.7AVRR931 pKa = 11.84QKK933 pKa = 11.2VNEE936 pKa = 4.08NPLYY940 pKa = 10.75APASEE945 pKa = 4.39HH946 pKa = 6.37VNVLLTRR953 pKa = 11.84TEE955 pKa = 4.6DD956 pKa = 3.39RR957 pKa = 11.84LVWKK961 pKa = 8.62TLAGDD966 pKa = 3.55PWIKK970 pKa = 10.52VLSNIPQGNFTATLEE985 pKa = 4.22EE986 pKa = 4.35WQEE989 pKa = 3.85EE990 pKa = 3.97HH991 pKa = 7.2DD992 pKa = 4.81KK993 pKa = 10.82IMKK996 pKa = 9.93VIEE999 pKa = 4.63GPAAPVDD1006 pKa = 3.69AFQNKK1011 pKa = 9.99ANVCWAKK1018 pKa = 10.88SLVPVLDD1025 pKa = 3.54TAGIRR1030 pKa = 11.84LTAEE1034 pKa = 3.76EE1035 pKa = 4.66WSTIITAFKK1044 pKa = 10.08EE1045 pKa = 3.75DD1046 pKa = 3.37RR1047 pKa = 11.84AYY1049 pKa = 11.34SPVVALNEE1057 pKa = 4.04ICTKK1061 pKa = 10.69YY1062 pKa = 10.96YY1063 pKa = 10.85GVDD1066 pKa = 3.45LDD1068 pKa = 4.44SGLFSAPKK1076 pKa = 9.6VSLYY1080 pKa = 10.84YY1081 pKa = 10.64EE1082 pKa = 4.16NNHH1085 pKa = 5.6WDD1087 pKa = 3.5NRR1089 pKa = 11.84PGGRR1093 pKa = 11.84MYY1095 pKa = 11.26GFNAATAARR1104 pKa = 11.84LEE1106 pKa = 4.13ARR1108 pKa = 11.84HH1109 pKa = 5.76TFLKK1113 pKa = 9.85GQWHH1117 pKa = 6.09TGKK1120 pKa = 9.98QAVIAEE1126 pKa = 4.36RR1127 pKa = 11.84KK1128 pKa = 8.5IQPLSVLDD1136 pKa = 3.61NVIPINRR1143 pKa = 11.84RR1144 pKa = 11.84LPHH1147 pKa = 6.57ALVAEE1152 pKa = 4.27YY1153 pKa = 8.56KK1154 pKa = 8.37TVKK1157 pKa = 9.98GSRR1160 pKa = 11.84VEE1162 pKa = 3.79WLVNKK1167 pKa = 9.64VRR1169 pKa = 11.84GYY1171 pKa = 10.55HH1172 pKa = 5.14VLLVSEE1178 pKa = 4.81YY1179 pKa = 11.04NLALPRR1185 pKa = 11.84RR1186 pKa = 11.84RR1187 pKa = 11.84VTWLSPLNVTGADD1200 pKa = 3.01RR1201 pKa = 11.84CYY1203 pKa = 10.89DD1204 pKa = 3.78LSLGLPADD1212 pKa = 3.92AGRR1215 pKa = 11.84FDD1217 pKa = 4.5LVFVNIHH1224 pKa = 4.31TEE1226 pKa = 4.0FRR1228 pKa = 11.84IHH1230 pKa = 7.58HH1231 pKa = 5.41YY1232 pKa = 7.31QQCVDD1237 pKa = 3.83HH1238 pKa = 6.91AMKK1241 pKa = 10.65LQMLGGDD1248 pKa = 4.2ALRR1251 pKa = 11.84LLKK1254 pKa = 10.65PGGSLLMRR1262 pKa = 11.84AYY1264 pKa = 10.4GYY1266 pKa = 11.07ADD1268 pKa = 4.41KK1269 pKa = 10.72ISEE1272 pKa = 4.33AVVSSLSRR1280 pKa = 11.84KK1281 pKa = 8.87FSSARR1286 pKa = 11.84VLRR1289 pKa = 11.84PDD1291 pKa = 3.49CVTSNTEE1298 pKa = 3.76VFLLFSNFDD1307 pKa = 3.31NGKK1310 pKa = 9.74RR1311 pKa = 11.84PSTLHH1316 pKa = 5.61QMNTKK1321 pKa = 10.39LSAVYY1326 pKa = 10.1AGEE1329 pKa = 4.06AMHH1332 pKa = 6.72TAGCAPSYY1340 pKa = 9.56RR1341 pKa = 11.84VKK1343 pKa = 10.66RR1344 pKa = 11.84ADD1346 pKa = 2.92IATCTEE1352 pKa = 3.92AAVVNAANARR1362 pKa = 11.84GTVGDD1367 pKa = 4.28GVCRR1371 pKa = 11.84AVAKK1375 pKa = 9.9KK1376 pKa = 9.48WPSAFKK1382 pKa = 10.75GAATPVGTIKK1392 pKa = 9.95TVMCGSYY1399 pKa = 10.31PVIHH1403 pKa = 6.65AVAPNFSATTEE1414 pKa = 4.15AEE1416 pKa = 4.08GDD1418 pKa = 3.59RR1419 pKa = 11.84EE1420 pKa = 3.87LAAVYY1425 pKa = 9.82RR1426 pKa = 11.84AVAAEE1431 pKa = 4.03VNRR1434 pKa = 11.84LSLSSVAIPLLSTGVFSGGRR1454 pKa = 11.84DD1455 pKa = 3.02RR1456 pKa = 11.84LQQSLNHH1463 pKa = 6.74LFTAMDD1469 pKa = 3.6ATDD1472 pKa = 4.21ADD1474 pKa = 3.8VTIYY1478 pKa = 10.82CRR1480 pKa = 11.84DD1481 pKa = 3.47KK1482 pKa = 10.78SWEE1485 pKa = 4.05KK1486 pKa = 10.56KK1487 pKa = 8.41IQEE1490 pKa = 4.7AIDD1493 pKa = 3.35MRR1495 pKa = 11.84TAVEE1499 pKa = 4.23LLNDD1503 pKa = 4.02DD1504 pKa = 4.63VEE1506 pKa = 4.51LTTDD1510 pKa = 4.22LVRR1513 pKa = 11.84VHH1515 pKa = 7.22PDD1517 pKa = 2.53SSLVGRR1523 pKa = 11.84KK1524 pKa = 9.54GYY1526 pKa = 8.43STTDD1530 pKa = 3.0GSLYY1534 pKa = 10.63SYY1536 pKa = 11.4FEE1538 pKa = 4.03GTKK1541 pKa = 10.16FNQAAIDD1548 pKa = 3.62MAEE1551 pKa = 4.9ILTLWPRR1558 pKa = 11.84LQEE1561 pKa = 4.21ANEE1564 pKa = 4.11QICLYY1569 pKa = 11.0ALGEE1573 pKa = 4.2TMDD1576 pKa = 4.97NIRR1579 pKa = 11.84SKK1581 pKa = 11.37CPVNDD1586 pKa = 4.19SDD1588 pKa = 5.14SSTPPRR1594 pKa = 11.84TVPCLCRR1601 pKa = 11.84YY1602 pKa = 10.52AMTAEE1607 pKa = 4.69RR1608 pKa = 11.84IARR1611 pKa = 11.84LRR1613 pKa = 11.84SHH1615 pKa = 5.78QVKK1618 pKa = 10.83SMVVCSSFPLPKK1630 pKa = 9.88YY1631 pKa = 10.39HH1632 pKa = 7.21VDD1634 pKa = 3.16GVQKK1638 pKa = 9.98VKK1640 pKa = 10.87CEE1642 pKa = 3.82KK1643 pKa = 10.81GLLFDD1648 pKa = 3.74PTVPSVVSPRR1658 pKa = 11.84KK1659 pKa = 9.08YY1660 pKa = 9.92AASTTDD1666 pKa = 2.7HH1667 pKa = 7.18SDD1669 pKa = 3.05RR1670 pKa = 11.84SLRR1673 pKa = 11.84GFDD1676 pKa = 5.28LDD1678 pKa = 3.33WTTDD1682 pKa = 3.36SSSTASDD1689 pKa = 3.5TMSLPSLQSCDD1700 pKa = 2.67IDD1702 pKa = 4.81SIYY1705 pKa = 11.04EE1706 pKa = 3.7PMAPIVVTADD1716 pKa = 3.19VHH1718 pKa = 6.11PEE1720 pKa = 3.55PAGIADD1726 pKa = 4.74LAADD1730 pKa = 3.93VHH1732 pKa = 6.51PEE1734 pKa = 3.94PADD1737 pKa = 3.56HH1738 pKa = 7.25VDD1740 pKa = 4.17LEE1742 pKa = 4.57NPIPPPRR1749 pKa = 11.84PKK1751 pKa = 10.1RR1752 pKa = 11.84AAYY1755 pKa = 9.15LASRR1759 pKa = 11.84AAEE1762 pKa = 3.89RR1763 pKa = 11.84PVPAPRR1769 pKa = 11.84KK1770 pKa = 6.39PTPAPRR1776 pKa = 11.84TAFRR1780 pKa = 11.84NKK1782 pKa = 10.32LPLTFGDD1789 pKa = 3.9FDD1791 pKa = 3.71EE1792 pKa = 6.23HH1793 pKa = 6.77EE1794 pKa = 4.31VDD1796 pKa = 4.77ALASGITFGDD1806 pKa = 3.4FDD1808 pKa = 4.35DD1809 pKa = 3.87VLRR1812 pKa = 11.84LGRR1815 pKa = 11.84AGAYY1819 pKa = 9.31IFSSDD1824 pKa = 3.4TGSGHH1829 pKa = 6.97LQQKK1833 pKa = 7.61SVRR1836 pKa = 11.84QHH1838 pKa = 6.07NLQCAQLDD1846 pKa = 3.81AVEE1849 pKa = 4.3EE1850 pKa = 4.2EE1851 pKa = 4.39KK1852 pKa = 10.26MYY1854 pKa = 10.41PPKK1857 pKa = 10.63LDD1859 pKa = 3.44TEE1861 pKa = 4.32RR1862 pKa = 11.84EE1863 pKa = 4.0KK1864 pKa = 11.42LLLLKK1869 pKa = 9.81MQMHH1873 pKa = 7.16PSEE1876 pKa = 4.58ANKK1879 pKa = 10.19SRR1881 pKa = 11.84YY1882 pKa = 7.27QSRR1885 pKa = 11.84KK1886 pKa = 7.62VEE1888 pKa = 4.03NMKK1891 pKa = 9.67ATVVDD1896 pKa = 4.27RR1897 pKa = 11.84LTSGARR1903 pKa = 11.84LYY1905 pKa = 10.44TGADD1909 pKa = 3.16VGRR1912 pKa = 11.84IPTYY1916 pKa = 9.62AVRR1919 pKa = 11.84YY1920 pKa = 5.92PRR1922 pKa = 11.84PVYY1925 pKa = 10.41SPTVIEE1931 pKa = 4.73RR1932 pKa = 11.84FSSPDD1937 pKa = 3.21VAIAACNEE1945 pKa = 4.08YY1946 pKa = 10.71LSRR1949 pKa = 11.84NYY1951 pKa = 8.4PTVASYY1957 pKa = 10.99QITDD1961 pKa = 3.64EE1962 pKa = 4.04YY1963 pKa = 11.39DD1964 pKa = 3.05AYY1966 pKa = 11.6LDD1968 pKa = 3.91MVDD1971 pKa = 5.75GSDD1974 pKa = 3.51SCLDD1978 pKa = 3.32RR1979 pKa = 11.84ATFCPAKK1986 pKa = 10.1LRR1988 pKa = 11.84CYY1990 pKa = 8.97PKK1992 pKa = 9.72HH1993 pKa = 6.07HH1994 pKa = 7.36AYY1996 pKa = 9.69HH1997 pKa = 5.9QPTVRR2002 pKa = 11.84SAVPSPFQNTLQNVLAAATKK2022 pKa = 9.91RR2023 pKa = 11.84NCNVTQMRR2031 pKa = 11.84EE2032 pKa = 4.17LPTMDD2037 pKa = 3.1SAVFNVEE2044 pKa = 3.6CFKK2047 pKa = 10.9RR2048 pKa = 11.84YY2049 pKa = 9.7ACSGEE2054 pKa = 3.93YY2055 pKa = 9.07WEE2057 pKa = 5.88EE2058 pKa = 4.15YY2059 pKa = 9.73AKK2061 pKa = 10.52QPIRR2065 pKa = 11.84ITTEE2069 pKa = 3.66NITTYY2074 pKa = 8.1VTKK2077 pKa = 10.96LKK2079 pKa = 10.51GPKK2082 pKa = 9.28AAALFAKK2089 pKa = 8.13THH2091 pKa = 5.99NLVPLQEE2098 pKa = 4.17VPMDD2102 pKa = 3.95RR2103 pKa = 11.84FTVDD2107 pKa = 2.78MKK2109 pKa = 11.01RR2110 pKa = 11.84DD2111 pKa = 3.64VKK2113 pKa = 9.76VTPGTKK2119 pKa = 8.49HH2120 pKa = 4.48TEE2122 pKa = 3.91EE2123 pKa = 4.12RR2124 pKa = 11.84PKK2126 pKa = 10.92VQVIQAAEE2134 pKa = 3.99PLATAYY2140 pKa = 10.83LCGIHH2145 pKa = 6.74RR2146 pKa = 11.84EE2147 pKa = 4.01LVRR2150 pKa = 11.84RR2151 pKa = 11.84LNAVLRR2157 pKa = 11.84PNVHH2161 pKa = 6.01TLFDD2165 pKa = 3.67MSAEE2169 pKa = 4.19DD2170 pKa = 3.84FDD2172 pKa = 6.61AIIASHH2178 pKa = 6.31FHH2180 pKa = 6.64PGDD2183 pKa = 3.57PVLEE2187 pKa = 3.96TDD2189 pKa = 3.41IASFDD2194 pKa = 3.58KK2195 pKa = 11.43SQDD2198 pKa = 3.29DD2199 pKa = 3.96SLALTGLMILEE2210 pKa = 4.67DD2211 pKa = 4.28LGVDD2215 pKa = 3.88QYY2217 pKa = 12.13LLDD2220 pKa = 5.14LIEE2223 pKa = 4.37AAFGEE2228 pKa = 4.67ISSCHH2233 pKa = 6.67LPTGTRR2239 pKa = 11.84FKK2241 pKa = 10.46FGAMMKK2247 pKa = 10.15SGMFLTLFINTVLNITIASRR2267 pKa = 11.84VLEE2270 pKa = 3.91QRR2272 pKa = 11.84LTDD2275 pKa = 3.72SACAAFIGDD2284 pKa = 4.09DD2285 pKa = 4.12NIVHH2289 pKa = 6.37GVISDD2294 pKa = 3.24KK2295 pKa = 11.39LMAEE2299 pKa = 4.95RR2300 pKa = 11.84CASWVNMEE2308 pKa = 4.29VKK2310 pKa = 10.31IIDD2313 pKa = 3.47AVMGEE2318 pKa = 4.34KK2319 pKa = 10.1PPYY2322 pKa = 8.65FCGGFIVFDD2331 pKa = 4.12SVTQTACRR2339 pKa = 11.84VSDD2342 pKa = 3.64PLKK2345 pKa = 10.81RR2346 pKa = 11.84LFKK2349 pKa = 10.55LGKK2352 pKa = 9.2PLTAEE2357 pKa = 4.49DD2358 pKa = 4.2KK2359 pKa = 10.5QDD2361 pKa = 3.36EE2362 pKa = 4.31DD2363 pKa = 3.63RR2364 pKa = 11.84RR2365 pKa = 11.84RR2366 pKa = 11.84ALSDD2370 pKa = 3.64EE2371 pKa = 3.9VSKK2374 pKa = 10.54WFRR2377 pKa = 11.84TGLGAEE2383 pKa = 4.45LEE2385 pKa = 4.48VALTSRR2391 pKa = 11.84YY2392 pKa = 8.32EE2393 pKa = 3.95VEE2395 pKa = 4.09GCKK2398 pKa = 10.54SILIAMATLARR2409 pKa = 11.84DD2410 pKa = 3.3IKK2412 pKa = 10.9AFKK2415 pKa = 10.14KK2416 pKa = 10.37LRR2418 pKa = 11.84GPVIHH2423 pKa = 7.18LYY2425 pKa = 10.45GGPRR2429 pKa = 11.84LVRR2432 pKa = 3.89

Molecular weight:
269.51 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>sp|P08411|POLN_SFV Polyprotein P1234 OS=Semliki forest virus OX=11033 PE=1 SV=2
MM1 pKa = 7.57NYY3 pKa = 9.73IPTQTFYY10 pKa = 11.22GRR12 pKa = 11.84RR13 pKa = 11.84WRR15 pKa = 11.84PRR17 pKa = 11.84PAARR21 pKa = 11.84PWPLQATPVAPVVPDD36 pKa = 4.5FQAQQMQQLISAVNALTMRR55 pKa = 11.84QNAIAPARR63 pKa = 11.84PPKK66 pKa = 9.73PKK68 pKa = 9.89KK69 pKa = 10.42KK70 pKa = 9.2KK71 pKa = 4.82TTKK74 pKa = 9.93PKK76 pKa = 10.59PKK78 pKa = 8.95TQPKK82 pKa = 9.21KK83 pKa = 10.74INGKK87 pKa = 6.47TQQQKK92 pKa = 10.93KK93 pKa = 9.73KK94 pKa = 10.68DD95 pKa = 3.53KK96 pKa = 10.97QADD99 pKa = 3.66KK100 pKa = 10.94KK101 pKa = 10.57KK102 pKa = 10.03KK103 pKa = 10.11KK104 pKa = 8.28PGKK107 pKa = 9.32RR108 pKa = 11.84EE109 pKa = 3.84RR110 pKa = 11.84MCMKK114 pKa = 10.12IEE116 pKa = 3.99NDD118 pKa = 4.0CIFEE122 pKa = 4.27VKK124 pKa = 10.55HH125 pKa = 5.59EE126 pKa = 4.41GKK128 pKa = 8.61VTGYY132 pKa = 10.98ACLVGDD138 pKa = 4.21KK139 pKa = 10.61VMKK142 pKa = 8.7PAHH145 pKa = 5.82VKK147 pKa = 10.64GVIDD151 pKa = 3.79NADD154 pKa = 3.68LAKK157 pKa = 10.68LAFKK161 pKa = 10.65KK162 pKa = 10.39SSKK165 pKa = 10.6YY166 pKa = 10.35DD167 pKa = 3.62LEE169 pKa = 4.45CAQIPVHH176 pKa = 5.68MRR178 pKa = 11.84SDD180 pKa = 3.26ASKK183 pKa = 8.2YY184 pKa = 6.47THH186 pKa = 6.76EE187 pKa = 4.89KK188 pKa = 10.56PEE190 pKa = 3.88GHH192 pKa = 6.34YY193 pKa = 9.58NWHH196 pKa = 6.8HH197 pKa = 6.34GAVQYY202 pKa = 11.02SGGRR206 pKa = 11.84FTIPTGAGKK215 pKa = 9.46PGDD218 pKa = 3.72SGRR221 pKa = 11.84PIFDD225 pKa = 3.06NKK227 pKa = 9.91GRR229 pKa = 11.84VVAIVLGGANEE240 pKa = 4.36GSRR243 pKa = 11.84TALSVVTWNKK253 pKa = 11.14DD254 pKa = 2.71MVTRR258 pKa = 11.84VTPEE262 pKa = 3.7GSEE265 pKa = 4.03EE266 pKa = 3.74WSAPLITAMCVLANATFPCFQPPCVPCCYY295 pKa = 10.03EE296 pKa = 4.03NNAEE300 pKa = 3.98ATLRR304 pKa = 11.84MLEE307 pKa = 4.44DD308 pKa = 3.5NVDD311 pKa = 3.39RR312 pKa = 11.84PGYY315 pKa = 10.45YY316 pKa = 10.31DD317 pKa = 4.58LLQAALTCRR326 pKa = 11.84NGTRR330 pKa = 11.84HH331 pKa = 5.8RR332 pKa = 11.84RR333 pKa = 11.84SVSQHH338 pKa = 4.65FNVYY342 pKa = 9.9KK343 pKa = 10.03ATRR346 pKa = 11.84PYY348 pKa = 10.02IAYY351 pKa = 10.04CADD354 pKa = 3.51CGAGHH359 pKa = 6.46SCHH362 pKa = 6.81SPVAIEE368 pKa = 4.08AVRR371 pKa = 11.84SEE373 pKa = 4.33ATDD376 pKa = 3.33GMLKK380 pKa = 9.73IQFSAQIGIDD390 pKa = 3.02KK391 pKa = 10.61SDD393 pKa = 3.05NHH395 pKa = 7.4DD396 pKa = 3.57YY397 pKa = 11.22TKK399 pKa = 10.33IRR401 pKa = 11.84YY402 pKa = 8.89ADD404 pKa = 3.14GHH406 pKa = 6.61AIEE409 pKa = 4.3NAVRR413 pKa = 11.84SSLKK417 pKa = 10.13VATSGDD423 pKa = 4.2CFVHH427 pKa = 5.62GTMGHH432 pKa = 6.92FILAKK437 pKa = 9.87CPPGEE442 pKa = 3.91FLQVSIQDD450 pKa = 3.12TRR452 pKa = 11.84NAVRR456 pKa = 11.84ACRR459 pKa = 11.84IQYY462 pKa = 9.47HH463 pKa = 7.02HH464 pKa = 7.76DD465 pKa = 3.69PQPVGRR471 pKa = 11.84EE472 pKa = 3.67KK473 pKa = 10.26FTIRR477 pKa = 11.84PHH479 pKa = 5.64YY480 pKa = 10.09GKK482 pKa = 10.36EE483 pKa = 4.26IPCTTYY489 pKa = 10.56QQTTAEE495 pKa = 4.3TVEE498 pKa = 4.69EE499 pKa = 4.81IDD501 pKa = 3.15MHH503 pKa = 6.15MPPDD507 pKa = 3.96TPDD510 pKa = 3.18RR511 pKa = 11.84TLLSQQSGNVKK522 pKa = 8.56ITVGGKK528 pKa = 8.7KK529 pKa = 9.85VKK531 pKa = 10.44YY532 pKa = 10.23NCTCGTGNVGTTNSDD547 pKa = 3.17MTINTCLIEE556 pKa = 4.06QCHH559 pKa = 5.71VSVTDD564 pKa = 3.47HH565 pKa = 6.9KK566 pKa = 10.65KK567 pKa = 8.74WQFNSPFVPRR577 pKa = 11.84ADD579 pKa = 3.41EE580 pKa = 3.93PARR583 pKa = 11.84KK584 pKa = 9.45GKK586 pKa = 8.83VHH588 pKa = 7.01IPFPLDD594 pKa = 3.73NITCRR599 pKa = 11.84VPMARR604 pKa = 11.84EE605 pKa = 3.63PTVIHH610 pKa = 6.73GKK612 pKa = 10.14RR613 pKa = 11.84EE614 pKa = 4.27VTLHH618 pKa = 6.31LHH620 pKa = 6.88PDD622 pKa = 3.19HH623 pKa = 6.99PTLFSYY629 pKa = 8.72RR630 pKa = 11.84TLGEE634 pKa = 4.09DD635 pKa = 3.51PQYY638 pKa = 10.93HH639 pKa = 5.81EE640 pKa = 4.77EE641 pKa = 4.19WVTAAVEE648 pKa = 4.08RR649 pKa = 11.84TIPVPVDD656 pKa = 2.65GMEE659 pKa = 4.04YY660 pKa = 9.84HH661 pKa = 7.16WGNNDD666 pKa = 3.59PVRR669 pKa = 11.84LWSQLTTEE677 pKa = 5.27GKK679 pKa = 8.23PHH681 pKa = 6.75GWPHH685 pKa = 6.65QIVQYY690 pKa = 10.99YY691 pKa = 8.98YY692 pKa = 10.96GLYY695 pKa = 9.55PAATVSAVVGMSLLALISIFASCYY719 pKa = 7.8MLVAARR725 pKa = 11.84SKK727 pKa = 10.98CLTPYY732 pKa = 11.25ALTPGAAVPWTLGILCCAPRR752 pKa = 11.84AHH754 pKa = 6.76AASVAEE760 pKa = 4.11TMAYY764 pKa = 10.36LWDD767 pKa = 3.63QNQALFWLEE776 pKa = 3.44FAAPVACILIITYY789 pKa = 8.79CLRR792 pKa = 11.84NVLCCCKK799 pKa = 10.39SLSFLVLLSLGATARR814 pKa = 11.84AYY816 pKa = 9.2EE817 pKa = 4.51HH818 pKa = 6.14STVMPNVVGFPYY830 pKa = 9.7KK831 pKa = 10.72AHH833 pKa = 6.97IEE835 pKa = 4.17RR836 pKa = 11.84PGYY839 pKa = 10.49SPLTLQMQVVEE850 pKa = 4.57TSLEE854 pKa = 3.89PTLNLEE860 pKa = 4.93YY861 pKa = 9.64ITCEE865 pKa = 4.01YY866 pKa = 8.89KK867 pKa = 10.34TVVPSPYY874 pKa = 10.04VKK876 pKa = 10.44CCGASEE882 pKa = 4.58CSTKK886 pKa = 10.47EE887 pKa = 4.07KK888 pKa = 10.25PDD890 pKa = 3.82YY891 pKa = 9.37QCKK894 pKa = 10.31VYY896 pKa = 10.29TGVYY900 pKa = 8.44PFMWGGAYY908 pKa = 9.35CFCDD912 pKa = 3.74SEE914 pKa = 4.29NTQLSEE920 pKa = 4.39AYY922 pKa = 9.93VDD924 pKa = 3.72RR925 pKa = 11.84SDD927 pKa = 4.22VCRR930 pKa = 11.84HH931 pKa = 5.59DD932 pKa = 3.53HH933 pKa = 6.08ASAYY937 pKa = 8.88KK938 pKa = 10.52AHH940 pKa = 6.24TASLKK945 pKa = 9.92AKK947 pKa = 9.38VRR949 pKa = 11.84VMYY952 pKa = 10.86GNVNQTVDD960 pKa = 3.35VYY962 pKa = 11.88VNGDD966 pKa = 3.5HH967 pKa = 7.05AVTIGGTQFIFGPLSSAWTPFDD989 pKa = 3.59NKK991 pKa = 10.09IVVYY995 pKa = 10.06KK996 pKa = 10.99DD997 pKa = 3.19EE998 pKa = 4.67VFNQDD1003 pKa = 3.32FPPYY1007 pKa = 10.63GSGQPGRR1014 pKa = 11.84FGDD1017 pKa = 3.53IQSRR1021 pKa = 11.84TVEE1024 pKa = 4.43SNDD1027 pKa = 3.38LYY1029 pKa = 11.6ANTALKK1035 pKa = 10.25LARR1038 pKa = 11.84PSPGMVHH1045 pKa = 6.16VPYY1048 pKa = 9.18TQTPSGFKK1056 pKa = 10.14YY1057 pKa = 9.05WLKK1060 pKa = 11.03EE1061 pKa = 3.53KK1062 pKa = 9.68GTALNTKK1069 pKa = 10.09APFGCQIKK1077 pKa = 8.87TNPVRR1082 pKa = 11.84AMNCAVGNIPVSMNLPDD1099 pKa = 3.47SAFTRR1104 pKa = 11.84IVEE1107 pKa = 4.22APTIIDD1113 pKa = 3.85LTCTVATCTHH1123 pKa = 6.42SSDD1126 pKa = 3.51FGGVLTLTYY1135 pKa = 8.8KK1136 pKa = 9.63TNKK1139 pKa = 10.3NGDD1142 pKa = 4.08CSVHH1146 pKa = 5.16SHH1148 pKa = 6.15SNVATLQEE1156 pKa = 3.85ATAKK1160 pKa = 10.48VKK1162 pKa = 9.41TAGKK1166 pKa = 8.12VTLHH1170 pKa = 6.7FSTASASPSFVVSLCSARR1188 pKa = 11.84ATCSASCEE1196 pKa = 4.12PPKK1199 pKa = 10.95DD1200 pKa = 3.7HH1201 pKa = 6.89IVPYY1205 pKa = 10.09AASHH1209 pKa = 6.14SNVVFPDD1216 pKa = 3.3MSGTALSWVQKK1227 pKa = 10.45ISGGLGAFAIGAILVLVVVTCIGLRR1252 pKa = 11.84RR1253 pKa = 3.66

Molecular weight:
138.02 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

3685

1253

2432

1842.5

203.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.227 ± 0.215

2.985 ± 0.495

5.346 ± 0.607

5.075 ± 0.588

3.202 ± 0.053

5.726 ± 0.433

2.904 ± 0.356

4.288 ± 0.06

6.133 ± 0.147

7.924 ± 1.135

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.198 ± 0.021

3.474 ± 0.256

6.106 ± 0.584

3.229 ± 0.399

5.482 ± 0.64

6.214 ± 0.087

7.273 ± 0.275

8.521 ± 0.129

1.085 ± 0.159

3.609 ± 0.27

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski