Amino acid dipepetide frequency for Bacillus subtilis (strain 168)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.85AlaAla: 7.85 ± 0.103	0.722AlaCys: 0.722 ± 0.026	4.221AlaAsp: 4.221 ± 0.051	5.634AlaGlu: 5.634 ± 0.08	3.69AlaPhe: 3.69 ± 0.066	6.138AlaGly: 6.138 ± 0.085	1.394AlaHis: 1.394 ± 0.034	5.492AlaIle: 5.492 ± 0.068	5.083AlaLys: 5.083 ± 0.068	7.732AlaLeu: 7.732 ± 0.075	1.963AlaMet: 1.963 ± 0.042	2.674AlaAsn: 2.674 ± 0.047	2.182AlaPro: 2.182 ± 0.039	2.226AlaGln: 2.226 ± 0.045	2.719AlaArg: 2.719 ± 0.05	4.527AlaSer: 4.527 ± 0.061	2.782AlaThr: 2.782 ± 0.059	6.536AlaVal: 6.536 ± 0.084	0.607AlaTrp: 0.607 ± 0.024	2.637AlaTyr: 2.637 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	0.529CysAla: 0.529 ± 0.023	0.119CysCys: 0.119 ± 0.011	0.366CysAsp: 0.366 ± 0.018	0.503CysGlu: 0.503 ± 0.022	0.404CysPhe: 0.404 ± 0.017	0.748CysGly: 0.748 ± 0.027	0.22CysHis: 0.22 ± 0.011	0.591CysIle: 0.591 ± 0.022	0.357CysLys: 0.357 ± 0.019	0.822CysLeu: 0.822 ± 0.026	0.227CysMet: 0.227 ± 0.015	0.239CysAsn: 0.239 ± 0.015	0.354CysPro: 0.354 ± 0.017	0.242CysGln: 0.242 ± 0.015	0.339CysArg: 0.339 ± 0.016	0.548CysSer: 0.548 ± 0.024	0.407CysThr: 0.407 ± 0.019	0.495CysVal: 0.495 ± 0.022	0.098CysTrp: 0.098 ± 0.01	0.263CysTyr: 0.263 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	3.43AspAla: 3.43 ± 0.059	0.391AspCys: 0.391 ± 0.018	2.611AspAsp: 2.611 ± 0.05	4.307AspGlu: 4.307 ± 0.064	2.248AspPhe: 2.248 ± 0.041	3.454AspGly: 3.454 ± 0.054	1.305AspHis: 1.305 ± 0.031	4.418AspIle: 4.418 ± 0.066	3.147AspLys: 3.147 ± 0.056	4.805AspLeu: 4.805 ± 0.063	1.544AspMet: 1.544 ± 0.039	1.562AspAsn: 1.562 ± 0.033	2.083AspPro: 2.083 ± 0.043	2.4AspGln: 2.4 ± 0.048	2.396AspArg: 2.396 ± 0.048	2.76AspSer: 2.76 ± 0.043	2.572AspThr: 2.572 ± 0.048	3.694AspVal: 3.694 ± 0.062	0.626AspTrp: 0.626 ± 0.023	2.095AspTyr: 2.095 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	5.726GluAla: 5.726 ± 0.087	0.45GluCys: 0.45 ± 0.019	3.808GluAsp: 3.808 ± 0.06	6.46GluGlu: 6.46 ± 0.106	2.388GluPhe: 2.388 ± 0.042	4.124GluGly: 4.124 ± 0.066	1.781GluHis: 1.781 ± 0.044	4.693GluIle: 4.693 ± 0.07	6.642GluLys: 6.642 ± 0.091	7.071GluLeu: 7.071 ± 0.093	2.129GluMet: 2.129 ± 0.035	3.354GluAsn: 3.354 ± 0.052	2.087GluPro: 2.087 ± 0.052	3.41GluGln: 3.41 ± 0.063	3.55GluArg: 3.55 ± 0.065	3.505GluSer: 3.505 ± 0.047	4.107GluThr: 4.107 ± 0.058	4.246GluVal: 4.246 ± 0.078	0.867GluTrp: 0.867 ± 0.03	2.205GluTyr: 2.205 ± 0.045	0.0GluXaa: 0.0 ± 0.0
Phe	3.163PheAla: 3.163 ± 0.063	0.4PheCys: 0.4 ± 0.018	2.36PheAsp: 2.36 ± 0.05	2.797PheGlu: 2.797 ± 0.059	2.449PhePhe: 2.449 ± 0.06	3.332PheGly: 3.332 ± 0.069	1.068PheHis: 1.068 ± 0.031	3.628PheIle: 3.628 ± 0.065	2.367PheLys: 2.367 ± 0.044	4.558PheLeu: 4.558 ± 0.084	1.185PheMet: 1.185 ± 0.033	1.559PheAsn: 1.559 ± 0.042	1.63PhePro: 1.63 ± 0.038	1.75PheGln: 1.75 ± 0.04	1.586PheArg: 1.586 ± 0.035	3.394PheSer: 3.394 ± 0.062	2.632PheThr: 2.632 ± 0.051	3.017PheVal: 3.017 ± 0.065	0.464PheTrp: 0.464 ± 0.023	1.655PheTyr: 1.655 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	5.105GlyAla: 5.105 ± 0.084	0.653GlyCys: 0.653 ± 0.025	3.269GlyAsp: 3.269 ± 0.053	4.432GlyGlu: 4.432 ± 0.063	3.444GlyPhe: 3.444 ± 0.061	4.964GlyGly: 4.964 ± 0.08	1.451GlyHis: 1.451 ± 0.036	5.757GlyIle: 5.757 ± 0.078	4.956GlyLys: 4.956 ± 0.065	6.655GlyLeu: 6.655 ± 0.093	2.1GlyMet: 2.1 ± 0.046	2.487GlyAsn: 2.487 ± 0.047	1.751GlyPro: 1.751 ± 0.041	2.25GlyGln: 2.25 ± 0.041	2.878GlyArg: 2.878 ± 0.048	4.251GlySer: 4.251 ± 0.061	4.192GlyThr: 4.192 ± 0.07	4.977GlyVal: 4.977 ± 0.074	0.801GlyTrp: 0.801 ± 0.029	2.75GlyTyr: 2.75 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	1.52HisAla: 1.52 ± 0.034	0.222HisCys: 0.222 ± 0.013	1.166HisAsp: 1.166 ± 0.032	1.478HisGlu: 1.478 ± 0.035	1.134HisPhe: 1.134 ± 0.029	1.392HisGly: 1.392 ± 0.035	0.765HisHis: 0.765 ± 0.033	1.777HisIle: 1.777 ± 0.041	1.162HisLys: 1.162 ± 0.036	2.196HisLeu: 2.196 ± 0.044	0.632HisMet: 0.632 ± 0.023	0.777HisAsn: 0.777 ± 0.03	1.261HisPro: 1.261 ± 0.032	0.972HisGln: 0.972 ± 0.024	0.883HisArg: 0.883 ± 0.025	1.382HisSer: 1.382 ± 0.036	1.249HisThr: 1.249 ± 0.03	1.57HisVal: 1.57 ± 0.035	0.239HisTrp: 0.239 ± 0.014	0.907HisTyr: 0.907 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.956IleAla: 5.956 ± 0.084	0.721IleCys: 0.721 ± 0.024	4.155IleAsp: 4.155 ± 0.06	5.377IleGlu: 5.377 ± 0.08	2.983IlePhe: 2.983 ± 0.062	5.866IleGly: 5.866 ± 0.074	1.742IleHis: 1.742 ± 0.042	5.125IleIle: 5.125 ± 0.089	4.509IleLys: 4.509 ± 0.077	6.47IleLeu: 6.47 ± 0.083	1.758IleMet: 1.758 ± 0.041	2.769IleAsn: 2.769 ± 0.057	3.184IlePro: 3.184 ± 0.049	2.764IleGln: 2.764 ± 0.046	3.005IleArg: 3.005 ± 0.054	5.11IleSer: 5.11 ± 0.07	4.108IleThr: 4.108 ± 0.057	5.144IleVal: 5.144 ± 0.068	0.693IleTrp: 0.693 ± 0.027	2.274IleTyr: 2.274 ± 0.045	0.0IleXaa: 0.0 ± 0.0
Lys	5.404LysAla: 5.404 ± 0.066	0.316LysCys: 0.316 ± 0.016	4.129LysAsp: 4.129 ± 0.072	6.861LysGlu: 6.861 ± 0.077	1.529LysPhe: 1.529 ± 0.033	4.778LysGly: 4.778 ± 0.061	1.546LysHis: 1.546 ± 0.036	4.024LysIle: 4.024 ± 0.057	6.481LysLys: 6.481 ± 0.098	5.835LysLeu: 5.835 ± 0.068	2.097LysMet: 2.097 ± 0.038	3.197LysAsn: 3.197 ± 0.055	2.487LysPro: 2.487 ± 0.051	3.558LysGln: 3.558 ± 0.053	3.622LysArg: 3.622 ± 0.055	3.625LysSer: 3.625 ± 0.066	4.34LysThr: 4.34 ± 0.058	4.149LysVal: 4.149 ± 0.068	0.874LysTrp: 0.874 ± 0.03	1.86LysTyr: 1.86 ± 0.046	0.0LysXaa: 0.0 ± 0.0
Leu	7.521LeuAla: 7.521 ± 0.082	0.775LeuCys: 0.775 ± 0.023	4.7LeuAsp: 4.7 ± 0.068	6.079LeuGlu: 6.079 ± 0.075	5.053LeuPhe: 5.053 ± 0.091	6.2LeuGly: 6.2 ± 0.084	2.197LeuHis: 2.197 ± 0.045	7.054LeuIle: 7.054 ± 0.094	6.773LeuLys: 6.773 ± 0.074	9.947LeuLeu: 9.947 ± 0.111	2.475LeuMet: 2.475 ± 0.045	3.966LeuAsn: 3.966 ± 0.06	3.938LeuPro: 3.938 ± 0.059	3.569LeuGln: 3.569 ± 0.057	3.57LeuArg: 3.57 ± 0.054	7.225LeuSer: 7.225 ± 0.081	5.789LeuThr: 5.789 ± 0.067	5.603LeuVal: 5.603 ± 0.072	0.854LeuTrp: 0.854 ± 0.033	3.283LeuTyr: 3.283 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	2.186MetAla: 2.186 ± 0.046	0.17MetCys: 0.17 ± 0.012	1.327MetAsp: 1.327 ± 0.034	1.738MetGlu: 1.738 ± 0.037	1.146MetPhe: 1.146 ± 0.032	1.633MetGly: 1.633 ± 0.045	0.438MetHis: 0.438 ± 0.021	2.213MetIle: 2.213 ± 0.044	2.756MetLys: 2.756 ± 0.051	2.763MetLeu: 2.763 ± 0.05	0.906MetMet: 0.906 ± 0.027	1.563MetAsn: 1.563 ± 0.039	1.069MetPro: 1.069 ± 0.029	0.872MetGln: 0.872 ± 0.026	1.049MetArg: 1.049 ± 0.028	1.757MetSer: 1.757 ± 0.037	1.791MetThr: 1.791 ± 0.04	1.596MetVal: 1.596 ± 0.036	0.175MetTrp: 0.175 ± 0.012	0.743MetTyr: 0.743 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	2.793AsnAla: 2.793 ± 0.044	0.269AsnCys: 0.269 ± 0.014	2.026AsnAsp: 2.026 ± 0.04	3.085AsnGlu: 3.085 ± 0.062	1.254AsnPhe: 1.254 ± 0.036	3.385AsnGly: 3.385 ± 0.063	0.965AsnHis: 0.965 ± 0.031	3.013AsnIle: 3.013 ± 0.056	2.675AsnLys: 2.675 ± 0.052	3.113AsnLeu: 3.113 ± 0.052	1.135AsnMet: 1.135 ± 0.033	1.503AsnAsn: 1.503 ± 0.045	1.869AsnPro: 1.869 ± 0.041	1.826AsnGln: 1.826 ± 0.043	1.955AsnArg: 1.955 ± 0.039	2.141AsnSer: 2.141 ± 0.043	2.052AsnThr: 2.052 ± 0.04	2.759AsnVal: 2.759 ± 0.051	0.501AsnTrp: 0.501 ± 0.023	1.22AsnTyr: 1.22 ± 0.034	0.0AsnXaa: 0.0 ± 0.0
Pro	2.766ProAla: 2.766 ± 0.05	0.232ProCys: 0.232 ± 0.014	2.336ProAsp: 2.336 ± 0.05	3.126ProGlu: 3.126 ± 0.058	2.087ProPhe: 2.087 ± 0.046	2.364ProGly: 2.364 ± 0.053	0.918ProHis: 0.918 ± 0.029	2.577ProIle: 2.577 ± 0.043	2.254ProLys: 2.254 ± 0.043	3.543ProLeu: 3.543 ± 0.065	0.805ProMet: 0.805 ± 0.03	1.521ProAsn: 1.521 ± 0.036	1.014ProPro: 1.014 ± 0.03	1.121ProGln: 1.121 ± 0.029	1.148ProArg: 1.148 ± 0.03	2.427ProSer: 2.427 ± 0.05	1.547ProThr: 1.547 ± 0.038	3.006ProVal: 3.006 ± 0.054	0.357ProTrp: 0.357 ± 0.016	1.491ProTyr: 1.491 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	3.074GlnAla: 3.074 ± 0.054	0.221GlnCys: 0.221 ± 0.012	1.779GlnAsp: 1.779 ± 0.041	2.747GlnGlu: 2.747 ± 0.05	1.595GlnPhe: 1.595 ± 0.034	2.012GlnGly: 2.012 ± 0.037	0.981GlnHis: 0.981 ± 0.03	2.573GlnIle: 2.573 ± 0.044	3.14GlnLys: 3.14 ± 0.056	3.966GlnLeu: 3.966 ± 0.064	1.254GlnMet: 1.254 ± 0.034	1.673GlnAsn: 1.673 ± 0.036	1.364GlnPro: 1.364 ± 0.037	1.867GlnGln: 1.867 ± 0.051	1.435GlnArg: 1.435 ± 0.04	2.268GlnSer: 2.268 ± 0.04	2.369GlnThr: 2.369 ± 0.044	2.25GlnVal: 2.25 ± 0.041	0.388GlnTrp: 0.388 ± 0.016	1.389GlnTyr: 1.389 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	2.598ArgAla: 2.598 ± 0.056	0.274ArgCys: 0.274 ± 0.016	2.039ArgAsp: 2.039 ± 0.041	3.151ArgGlu: 3.151 ± 0.058	2.055ArgPhe: 2.055 ± 0.042	2.256ArgGly: 2.256 ± 0.048	0.987ArgHis: 0.987 ± 0.03	3.031ArgIle: 3.031 ± 0.043	3.208ArgLys: 3.208 ± 0.057	4.201ArgLeu: 4.201 ± 0.065	1.382ArgMet: 1.382 ± 0.036	1.75ArgAsn: 1.75 ± 0.041	1.361ArgPro: 1.361 ± 0.036	1.634ArgGln: 1.634 ± 0.036	1.925ArgArg: 1.925 ± 0.045	2.361ArgSer: 2.361 ± 0.05	2.203ArgThr: 2.203 ± 0.043	2.53ArgVal: 2.53 ± 0.054	0.412ArgTrp: 0.412 ± 0.021	1.559ArgTyr: 1.559 ± 0.032	0.001ArgXaa: 0.001 ± 0.001
Ser	4.824SerAla: 4.824 ± 0.066	0.492SerCys: 0.492 ± 0.026	3.064SerAsp: 3.064 ± 0.057	4.074SerGlu: 4.074 ± 0.057	3.416SerPhe: 3.416 ± 0.058	4.93SerGly: 4.93 ± 0.057	1.297SerHis: 1.297 ± 0.036	4.827SerIle: 4.827 ± 0.064	3.891SerLys: 3.891 ± 0.063	6.398SerLeu: 6.398 ± 0.08	1.812SerMet: 1.812 ± 0.034	2.19SerAsn: 2.19 ± 0.045	2.289SerPro: 2.289 ± 0.041	2.101SerGln: 2.101 ± 0.039	2.39SerArg: 2.39 ± 0.05	4.34SerSer: 4.34 ± 0.085	2.863SerThr: 2.863 ± 0.057	4.461SerVal: 4.461 ± 0.065	0.659SerTrp: 0.659 ± 0.022	2.46SerTyr: 2.46 ± 0.045	0.0SerXaa: 0.0 ± 0.0
Thr	4.931ThrAla: 4.931 ± 0.072	0.412ThrCys: 0.412 ± 0.021	2.921ThrAsp: 2.921 ± 0.053	3.751ThrGlu: 3.751 ± 0.055	2.559ThrPhe: 2.559 ± 0.05	4.357ThrGly: 4.357 ± 0.053	1.084ThrHis: 1.084 ± 0.03	4.197ThrIle: 4.197 ± 0.058	3.416ThrLys: 3.416 ± 0.061	5.094ThrLeu: 5.094 ± 0.062	1.278ThrMet: 1.278 ± 0.034	2.111ThrAsn: 2.111 ± 0.04	2.24ThrPro: 2.24 ± 0.045	1.425ThrGln: 1.425 ± 0.033	1.765ThrArg: 1.765 ± 0.035	3.407ThrSer: 3.407 ± 0.057	2.455ThrThr: 2.455 ± 0.049	4.289ThrVal: 4.289 ± 0.064	0.492ThrTrp: 0.492 ± 0.021	2.039ThrTyr: 2.039 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	4.43ValAla: 4.43 ± 0.071	0.654ValCys: 0.654 ± 0.024	3.03ValAsp: 3.03 ± 0.054	3.867ValGlu: 3.867 ± 0.06	3.286ValPhe: 3.286 ± 0.061	4.017ValGly: 4.017 ± 0.068	1.435ValHis: 1.435 ± 0.037	5.56ValIle: 5.56 ± 0.085	4.937ValLys: 4.937 ± 0.071	7.116ValLeu: 7.116 ± 0.089	1.943ValMet: 1.943 ± 0.035	2.893ValAsn: 2.893 ± 0.046	2.754ValPro: 2.754 ± 0.051	2.4ValGln: 2.4 ± 0.042	2.654ValArg: 2.654 ± 0.048	4.959ValSer: 4.959 ± 0.062	4.259ValThr: 4.259 ± 0.057	4.253ValVal: 4.253 ± 0.071	0.686ValTrp: 0.686 ± 0.027	2.374ValTyr: 2.374 ± 0.044	0.0ValXaa: 0.0 ± 0.0
Trp	0.676TrpAla: 0.676 ± 0.028	0.086TrpCys: 0.086 ± 0.009	0.518TrpAsp: 0.518 ± 0.022	0.619TrpGlu: 0.619 ± 0.021	0.539TrpPhe: 0.539 ± 0.023	0.659TrpGly: 0.659 ± 0.026	0.23TrpHis: 0.23 ± 0.014	0.822TrpIle: 0.822 ± 0.028	0.797TrpLys: 0.797 ± 0.025	1.127TrpLeu: 1.127 ± 0.037	0.349TrpMet: 0.349 ± 0.016	0.499TrpAsn: 0.499 ± 0.019	0.292TrpPro: 0.292 ± 0.016	0.351TrpGln: 0.351 ± 0.019	0.429TrpArg: 0.429 ± 0.018	0.65TrpSer: 0.65 ± 0.025	0.559TrpThr: 0.559 ± 0.021	0.653TrpVal: 0.653 ± 0.024	0.131TrpTrp: 0.131 ± 0.01	0.344TrpTyr: 0.344 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.326TyrAla: 2.326 ± 0.036	0.29TyrCys: 0.29 ± 0.014	2.02TyrAsp: 2.02 ± 0.05	2.518TyrGlu: 2.518 ± 0.049	1.738TyrPhe: 1.738 ± 0.038	2.515TyrGly: 2.515 ± 0.049	0.882TyrHis: 0.882 ± 0.025	2.34TyrIle: 2.34 ± 0.046	2.162TyrLys: 2.162 ± 0.044	3.159TyrLeu: 3.159 ± 0.057	0.916TyrMet: 0.916 ± 0.025	1.25TyrAsn: 1.25 ± 0.034	1.431TyrPro: 1.431 ± 0.036	1.605TyrGln: 1.605 ± 0.037	1.622TyrArg: 1.622 ± 0.043	2.142TyrSer: 2.142 ± 0.038	1.949TyrThr: 1.949 ± 0.042	2.278TyrVal: 2.278 ± 0.044	0.406TyrTrp: 0.406 ± 0.02	1.353TyrTyr: 1.353 ± 0.038	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.001	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4267 proteins (1236478 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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