Vibrio proteolyticus NBRC 13287

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio; Vibrio proteolyticus

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4347 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U3A5L8|U3A5L8_VIBPR Ancillary SecYEG translocon subunit OS=Vibrio proteolyticus NBRC 13287 OX=1219065 GN=VPR01S_18_00340 PE=3 SV=1
MM1 pKa = 7.63GRR3 pKa = 11.84RR4 pKa = 11.84FKK6 pKa = 11.02NGYY9 pKa = 8.82LRR11 pKa = 11.84AAVVLMLSTLGLVGCNSDD29 pKa = 3.62SDD31 pKa = 5.1DD32 pKa = 4.17DD33 pKa = 4.59VSSQVQALSFNVTNTTDD50 pKa = 3.68DD51 pKa = 3.89TPLSNLEE58 pKa = 3.69VRR60 pKa = 11.84FVFDD64 pKa = 4.84VSNQSQDD71 pKa = 2.97FRR73 pKa = 11.84VSVTDD78 pKa = 3.16VSGRR82 pKa = 11.84EE83 pKa = 3.61VAGYY87 pKa = 10.96VDD89 pKa = 3.77GTAYY93 pKa = 9.99QSNVLLVPDD102 pKa = 4.27SGIVPISISAFNVSSSGDD120 pKa = 3.34SRR122 pKa = 11.84ASLTLVTHH130 pKa = 6.51GKK132 pKa = 10.76GFFSNSQYY140 pKa = 11.03FDD142 pKa = 3.37LTQTSMEE149 pKa = 3.71TSYY152 pKa = 10.29WVEE155 pKa = 3.93VMPKK159 pKa = 10.11SSSDD163 pKa = 3.8DD164 pKa = 3.36VATAYY169 pKa = 9.94AAQIVAMSGSSTASTLTLSTPIQSTTTSGGQILDD203 pKa = 3.74GASASLTIPAGTTLLAEE220 pKa = 5.11DD221 pKa = 5.0GSAFVPVGDD230 pKa = 3.73ITASLMMFSADD241 pKa = 3.46PQGIADD247 pKa = 4.27ADD249 pKa = 3.99NNPLYY254 pKa = 10.38LFPGGLSPQEE264 pKa = 4.03VTGDD268 pKa = 3.83LPADD272 pKa = 3.24IRR274 pKa = 11.84SATGLTFISAGFVAIEE290 pKa = 4.49LEE292 pKa = 4.39DD293 pKa = 3.54EE294 pKa = 5.0AGNQVKK300 pKa = 10.23GFDD303 pKa = 3.59GSGIRR308 pKa = 11.84LTFDD312 pKa = 3.28VPKK315 pKa = 10.27STTNPNTGAPLSLNDD330 pKa = 3.11QTIPIWSYY338 pKa = 11.28TDD340 pKa = 3.31TTGKK344 pKa = 7.78WHH346 pKa = 6.47YY347 pKa = 9.49EE348 pKa = 4.01GEE350 pKa = 4.06ASIVKK355 pKa = 9.83EE356 pKa = 3.96NSDD359 pKa = 3.68TFTLSKK365 pKa = 10.67QITHH369 pKa = 7.01LSYY372 pKa = 11.81YY373 pKa = 10.07NLDD376 pKa = 3.32WYY378 pKa = 10.6DD379 pKa = 3.41QDD381 pKa = 4.42RR382 pKa = 11.84CKK384 pKa = 10.98LDD386 pKa = 3.39INVVDD391 pKa = 5.26QNGEE395 pKa = 4.02PNNQKK400 pKa = 10.18LRR402 pKa = 11.84LSFAKK407 pKa = 10.58AGGGWAYY414 pKa = 9.84KK415 pKa = 10.03PSGWGDD421 pKa = 3.32NPEE424 pKa = 3.87KK425 pKa = 11.14LEE427 pKa = 4.1INRR430 pKa = 11.84VPAFAGHH437 pKa = 6.84FDD439 pKa = 4.39LLDD442 pKa = 3.77SQGNSLLASIEE453 pKa = 3.84VDD455 pKa = 3.53SQVTSVASGDD465 pKa = 3.54TGLDD469 pKa = 3.15LDD471 pKa = 5.0DD472 pKa = 4.66FCFGLSGDD480 pKa = 3.72NTKK483 pKa = 10.66SLKK486 pKa = 9.52ATLNITNPPTIDD498 pKa = 3.15IQPTLKK504 pKa = 10.44LVCPTNTSVAQAVTSGRR521 pKa = 11.84YY522 pKa = 8.37YY523 pKa = 10.82LYY525 pKa = 10.42SGYY528 pKa = 10.49SYY530 pKa = 10.52EE531 pKa = 4.27SSGEE535 pKa = 3.77ISGDD539 pKa = 3.54TLSLTNLLQDD549 pKa = 3.38GLYY552 pKa = 10.37RR553 pKa = 11.84LYY555 pKa = 11.0YY556 pKa = 10.51YY557 pKa = 10.8GGQTWGQVEE566 pKa = 4.74FTASANLDD574 pKa = 4.02EE575 pKa = 4.72IQLMSLEE582 pKa = 4.62LCDD585 pKa = 6.43KK586 pKa = 10.52IDD588 pKa = 3.25QTVNVRR594 pKa = 11.84LVCLDD599 pKa = 3.63DD600 pKa = 3.81QQDD603 pKa = 3.78VTRR606 pKa = 11.84EE607 pKa = 3.73KK608 pKa = 10.57AAPSAYY614 pKa = 8.73YY615 pKa = 9.79WMYY618 pKa = 9.52NQDD621 pKa = 3.18YY622 pKa = 9.82GQYY625 pKa = 10.73LWGQVDD631 pKa = 3.88EE632 pKa = 5.96DD633 pKa = 3.92GTAQEE638 pKa = 4.28SRR640 pKa = 11.84AVDD643 pKa = 3.52TVQYY647 pKa = 10.01EE648 pKa = 4.16GSAYY652 pKa = 10.1VRR654 pKa = 11.84LDD656 pKa = 3.14NQYY659 pKa = 10.42YY660 pKa = 7.82WGEE663 pKa = 4.14RR664 pKa = 11.84QTVTASDD671 pKa = 3.8SQPVVFDD678 pKa = 3.24IALPSDD684 pKa = 3.31HH685 pKa = 6.69TFCSEE690 pKa = 3.73VSTVDD695 pKa = 3.33YY696 pKa = 11.07SGSTLVIADD705 pKa = 3.95TQGMDD710 pKa = 3.0IAATTTLTVTLKK722 pKa = 11.09DD723 pKa = 4.43GDD725 pKa = 4.04NQDD728 pKa = 3.72YY729 pKa = 8.01TQSGGTLEE737 pKa = 4.65LASSPSGLVFTDD749 pKa = 2.85ITDD752 pKa = 3.95NADD755 pKa = 3.05GTYY758 pKa = 10.32SATVSATDD766 pKa = 3.27AGSYY770 pKa = 8.84TISATVGGEE779 pKa = 4.16VLSNTAPLTITAVDD793 pKa = 3.71TSLSAVSPDD802 pKa = 3.11TVAQDD807 pKa = 3.38EE808 pKa = 4.78GSTTNVTLTLRR819 pKa = 11.84QADD822 pKa = 3.82NSVVGHH828 pKa = 6.78GGHH831 pKa = 6.22TVTATGLAIDD841 pKa = 4.74FGLVSVATTDD851 pKa = 4.07HH852 pKa = 7.76DD853 pKa = 4.61DD854 pKa = 3.53GTYY857 pKa = 9.89TLAVTCDD864 pKa = 2.87NGFNGSQDD872 pKa = 2.76INVNVNAVVVDD883 pKa = 3.98TLSVSCNSII892 pKa = 3.23

Molecular weight: 94.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U2ZMX6|U2ZMX6_VIBPR Cardiolipin synthase A OS=Vibrio proteolyticus NBRC 13287 OX=1219065 GN=cls PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.5RR3 pKa = 11.84TFQPTVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 7.65RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.44NGRR28 pKa = 11.84KK29 pKa = 9.39VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.74GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSKK44 pKa = 10.84

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4347

0

4347

1389250

39

3580

319.6

35.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.099 ± 0.038

1.042 ± 0.014

5.576 ± 0.039

6.078 ± 0.037

3.975 ± 0.026

6.868 ± 0.038

2.377 ± 0.02

5.887 ± 0.034

4.72 ± 0.033

10.579 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.713 ± 0.021

3.863 ± 0.024

4.052 ± 0.026

4.9 ± 0.035

4.901 ± 0.031

6.443 ± 0.039

5.353 ± 0.031

7.216 ± 0.031

1.302 ± 0.017

3.054 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski