Berne virus (BEV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Tornidovirineae; Tobaniviridae; Torovirinae; Torovirus; Renitovirus; Equine torovirus

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q65482|Q65482_BEV 9.8K protein OS=Berne virus OX=11156 PE=2 SV=1
MM1 pKa = 7.38NFTVPVQAIQSIWSVGKK18 pKa = 10.6EE19 pKa = 3.81SDD21 pKa = 3.61DD22 pKa = 5.52AIAEE26 pKa = 4.09ACKK29 pKa = 10.22PPFCIYY35 pKa = 10.56FSKK38 pKa = 9.27KK39 pKa = 8.29TPYY42 pKa = 10.01TVTNGSNADD51 pKa = 3.59HH52 pKa = 7.21GDD54 pKa = 3.69DD55 pKa = 4.19EE56 pKa = 4.76VRR58 pKa = 11.84QMMRR62 pKa = 11.84GLLYY66 pKa = 10.81NSSCISAQGHH76 pKa = 5.39TPLALYY82 pKa = 8.17STAMLYY88 pKa = 10.35PPMYY92 pKa = 9.66GSCPQYY98 pKa = 11.31VKK100 pKa = 10.95LFDD103 pKa = 4.06GSGSEE108 pKa = 4.27SVDD111 pKa = 3.84VISSSYY117 pKa = 10.38FVATWVLLVVVIILVFIIISFCISNN142 pKa = 3.47

Molecular weight: 15.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P23052|SPIKE_BEV Spike glycoprotein OS=Berne virus OX=11156 GN=S PE=2 SV=1
MM1 pKa = 7.76NSMLNPNAVPFQPSPQVVALPMQYY25 pKa = 10.53PSGFSSGYY33 pKa = 9.4RR34 pKa = 11.84RR35 pKa = 11.84QRR37 pKa = 11.84DD38 pKa = 3.29PAFRR42 pKa = 11.84PMFRR46 pKa = 11.84RR47 pKa = 11.84QNNGNQNRR55 pKa = 11.84SRR57 pKa = 11.84QNRR60 pKa = 11.84QRR62 pKa = 11.84LQNNNRR68 pKa = 11.84GNNRR72 pKa = 11.84NRR74 pKa = 11.84NQFNRR79 pKa = 11.84RR80 pKa = 11.84QNQPSQSMSFEE91 pKa = 4.02QQLLLMANEE100 pKa = 3.95TAYY103 pKa = 10.72AATYY107 pKa = 9.33PSDD110 pKa = 3.72MQNIAPTKK118 pKa = 9.25LVKK121 pKa = 9.4IAKK124 pKa = 8.54RR125 pKa = 11.84AAMQIVSGHH134 pKa = 5.28ATVEE138 pKa = 4.05ISNGTEE144 pKa = 3.77DD145 pKa = 3.34SNQRR149 pKa = 11.84VATFTIKK156 pKa = 10.74VVMNN160 pKa = 4.55

Molecular weight: 18.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

13630

88

6857

1947.1

222.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.637 ± 0.432

3.023 ± 0.147

5.018 ± 0.478

4.637 ± 0.607

6.508 ± 0.354

4.615 ± 0.073

2.267 ± 0.142

4.974 ± 0.543

5.51 ± 0.751

9.626 ± 0.21

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.238 ± 0.18

4.842 ± 0.324

4.615 ± 0.184

5.385 ± 0.251

3.243 ± 0.277

7.572 ± 0.625

5.884 ± 0.53

9.068 ± 0.376

1.453 ± 0.114

4.886 ± 0.306
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski