Halorubrum pleomorphic virus 2

Taxonomy: Viruses; Monodnaviria; Trapavirae; Saleviricota; Huolimaviricetes; Haloruvirales; Pleolipoviridae; Alphapleolipovirus; Alphapleolipovirus HRPV2

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9ABM0|H9ABM0_9VIRU ORF6 OS=Halorubrum pleomorphic virus 2 OX=1156719 GN=ORF6 PE=4 SV=1
MM1 pKa = 7.55GRR3 pKa = 11.84RR4 pKa = 11.84TFVKK8 pKa = 10.51GLGAAGAATVGLSVDD23 pKa = 4.34DD24 pKa = 5.68GFAQDD29 pKa = 3.91AQAIAPLVGVGLAAGAVGVGWALRR53 pKa = 11.84EE54 pKa = 3.93FEE56 pKa = 4.85IVGSDD61 pKa = 3.65APPEE65 pKa = 4.3GLTADD70 pKa = 3.62ALKK73 pKa = 10.31QQVYY77 pKa = 7.21QTAKK81 pKa = 8.92TRR83 pKa = 11.84KK84 pKa = 7.73STNASTIVDD93 pKa = 3.75NQNILDD99 pKa = 4.23GVKK102 pKa = 8.61HH103 pKa = 5.26TAYY106 pKa = 9.75TDD108 pKa = 3.32AKK110 pKa = 9.46IAAIEE115 pKa = 3.95EE116 pKa = 4.46LNAGSAEE123 pKa = 4.21SAVLDD128 pKa = 4.02AATTEE133 pKa = 4.3VNSYY137 pKa = 8.69LTTVQSNFLKK147 pKa = 9.59TWNEE151 pKa = 3.9SVAEE155 pKa = 4.06LDD157 pKa = 4.39SILSTVVNHH166 pKa = 6.72PDD168 pKa = 2.91IGKK171 pKa = 10.49GDD173 pKa = 3.92VFLMLNGSDD182 pKa = 3.54NTIEE186 pKa = 5.01DD187 pKa = 4.15LLANPSGSTDD197 pKa = 3.02ATSFTLADD205 pKa = 3.87GTTMSVGTVEE215 pKa = 4.02VDD217 pKa = 2.94RR218 pKa = 11.84GTEE221 pKa = 3.77SYY223 pKa = 11.09YY224 pKa = 10.34YY225 pKa = 10.72DD226 pKa = 3.98PMSGLVGDD234 pKa = 5.65LGDD237 pKa = 4.7LKK239 pKa = 11.3NGGPTVQYY247 pKa = 10.7DD248 pKa = 3.95GNSLVYY254 pKa = 10.65LNASNWKK261 pKa = 9.34PIYY264 pKa = 10.52DD265 pKa = 3.79EE266 pKa = 4.63MDD268 pKa = 3.1TVLQNVRR275 pKa = 11.84SGISTWVSNVYY286 pKa = 11.0GDD288 pKa = 3.81VQSGEE293 pKa = 4.29IEE295 pKa = 4.27VSDD298 pKa = 3.65LVTPRR303 pKa = 11.84EE304 pKa = 4.0RR305 pKa = 11.84AAMMAQEE312 pKa = 4.65EE313 pKa = 4.97GMSQAIADD321 pKa = 5.12LIALNVPVDD330 pKa = 3.84AEE332 pKa = 4.23RR333 pKa = 11.84EE334 pKa = 4.21ATITIQDD341 pKa = 3.56TGATLPGTFALTDD354 pKa = 3.64ASDD357 pKa = 4.16GPLEE361 pKa = 4.31SGKK364 pKa = 9.08TYY366 pKa = 10.79DD367 pKa = 3.71PSTFSGDD374 pKa = 3.59VYY376 pKa = 9.75FTADD380 pKa = 3.14MSLVEE385 pKa = 4.73GDD387 pKa = 2.89WTAYY391 pKa = 9.88QSGVDD396 pKa = 3.85GGNVTLTSEE405 pKa = 4.71PYY407 pKa = 10.31SGTAVEE413 pKa = 5.43LNTAANEE420 pKa = 4.25TVAVDD425 pKa = 3.73AGNWTATGNGTWYY438 pKa = 10.52HH439 pKa = 6.33DD440 pKa = 3.71VSPEE444 pKa = 4.05LEE446 pKa = 3.93TDD448 pKa = 2.85ITSIEE453 pKa = 4.21SARR456 pKa = 11.84FLSTAEE462 pKa = 3.68QTQYY466 pKa = 9.94EE467 pKa = 4.64TIQLQGSFTIDD478 pKa = 3.09KK479 pKa = 8.46LTNTQTGEE487 pKa = 4.16EE488 pKa = 4.26VTATSFDD495 pKa = 3.41SSEE498 pKa = 3.79PHH500 pKa = 6.37TDD502 pKa = 2.71SNYY505 pKa = 8.54ITQEE509 pKa = 3.3EE510 pKa = 4.64WDD512 pKa = 3.56QLEE515 pKa = 4.22QQNKK519 pKa = 8.28EE520 pKa = 4.17LIEE523 pKa = 4.64KK524 pKa = 10.22YY525 pKa = 9.73EE526 pKa = 3.97QSQSGGGLDD535 pKa = 4.4LGQFDD540 pKa = 4.01MFGIPGEE547 pKa = 4.08IVAVGVAALVGLGVLGNNN565 pKa = 4.05

Molecular weight: 59.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9ABL7|H9ABL7_9VIRU ORF3 OS=Halorubrum pleomorphic virus 2 OX=1156719 GN=ORF3 PE=4 SV=1
MM1 pKa = 7.63EE2 pKa = 4.65EE3 pKa = 3.72RR4 pKa = 11.84AKK6 pKa = 10.36QHH8 pKa = 5.94EE9 pKa = 4.74SPAARR14 pKa = 11.84AASYY18 pKa = 9.93PEE20 pKa = 3.92RR21 pKa = 11.84RR22 pKa = 11.84AKK24 pKa = 10.24RR25 pKa = 11.84AEE27 pKa = 4.12GYY29 pKa = 10.1RR30 pKa = 11.84DD31 pKa = 3.19GSRR34 pKa = 11.84RR35 pKa = 11.84GRR37 pKa = 11.84QPGPASWSEE46 pKa = 3.56RR47 pKa = 11.84RR48 pKa = 11.84EE49 pKa = 3.95ISEE52 pKa = 3.84HH53 pKa = 4.24QQRR56 pKa = 11.84EE57 pKa = 4.08LRR59 pKa = 11.84GEE61 pKa = 3.78EE62 pKa = 4.01TRR64 pKa = 11.84RR65 pKa = 11.84QDD67 pKa = 3.59EE68 pKa = 4.5TRR70 pKa = 11.84MSTSSTRR77 pKa = 11.84LL78 pKa = 3.2

Molecular weight: 9.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

3091

65

655

206.1

22.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.511 ± 0.6

1.003 ± 0.319

8.541 ± 1.147

6.632 ± 0.688

3.009 ± 0.356

8.25 ± 0.572

1.715 ± 0.382

3.365 ± 0.346

2.782 ± 0.479

9.059 ± 0.788

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.844 ± 0.212

2.653 ± 0.459

4.206 ± 0.377

3.235 ± 0.34

7.603 ± 1.216

7.667 ± 0.69

6.923 ± 0.723

7.732 ± 0.746

1.779 ± 0.183

2.491 ± 0.442
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski