Faeces associated gemycircularvirus 22

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus bovas1; Bovine associated gemycircularvirus 1

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A161J5P3|A0A161J5P3_9VIRU RepA OS=Faeces associated gemycircularvirus 22 OX=1843742 PE=3 SV=1
MM1 pKa = 7.81PFHH4 pKa = 6.86FGARR8 pKa = 11.84YY9 pKa = 9.91ALITYY14 pKa = 7.36AQCNALDD21 pKa = 3.87GFRR24 pKa = 11.84VMDD27 pKa = 4.02HH28 pKa = 6.63FSGLGAEE35 pKa = 4.62CIVGRR40 pKa = 11.84EE41 pKa = 3.86VHH43 pKa = 6.72ADD45 pKa = 3.32GGIHH49 pKa = 6.0LHH51 pKa = 6.36CFIDD55 pKa = 4.11FGRR58 pKa = 11.84KK59 pKa = 7.81FRR61 pKa = 11.84SRR63 pKa = 11.84RR64 pKa = 11.84TDD66 pKa = 2.64IFDD69 pKa = 3.14VDD71 pKa = 3.53GRR73 pKa = 11.84HH74 pKa = 6.16PNIAPSYY81 pKa = 6.47GTPWRR86 pKa = 11.84GYY88 pKa = 10.77DD89 pKa = 3.55YY90 pKa = 10.79AIKK93 pKa = 10.71DD94 pKa = 3.36GDD96 pKa = 4.29VICGGLEE103 pKa = 3.73RR104 pKa = 11.84PEE106 pKa = 4.01EE107 pKa = 4.12PRR109 pKa = 11.84SKK111 pKa = 10.46RR112 pKa = 11.84VTKK115 pKa = 10.54DD116 pKa = 2.49WDD118 pKa = 3.16PWTEE122 pKa = 3.55ITNARR127 pKa = 11.84DD128 pKa = 3.58RR129 pKa = 11.84EE130 pKa = 4.49HH131 pKa = 6.41FWEE134 pKa = 4.87LVHH137 pKa = 8.03HH138 pKa = 7.16LDD140 pKa = 4.88PKK142 pKa = 10.88AAACNYY148 pKa = 8.82GQLAKK153 pKa = 10.78YY154 pKa = 10.48ADD156 pKa = 3.32WRR158 pKa = 11.84FAAKK162 pKa = 9.95PPVYY166 pKa = 9.39EE167 pKa = 4.2SPGGIEE173 pKa = 4.29FVGGDD178 pKa = 3.31VDD180 pKa = 6.46GRR182 pKa = 11.84DD183 pKa = 3.34AWCDD187 pKa = 3.17QSGIRR192 pKa = 11.84SGDD195 pKa = 3.6PLIGTSVRR203 pKa = 11.84SPRR206 pKa = 11.84LPSSGTIGGVCLGLAPQGGPYY227 pKa = 9.23TPPDD231 pKa = 3.56GAEE234 pKa = 4.08TSLSRR239 pKa = 11.84CNANCRR245 pKa = 3.61

Molecular weight: 27.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A161IVT0|A0A161IVT0_9VIRU Replication associated protein OS=Faeces associated gemycircularvirus 22 OX=1843742 PE=3 SV=1
MM1 pKa = 7.7ARR3 pKa = 11.84LRR5 pKa = 11.84YY6 pKa = 9.11VKK8 pKa = 9.88RR9 pKa = 11.84RR10 pKa = 11.84YY11 pKa = 9.1RR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 8.32PNSRR18 pKa = 11.84RR19 pKa = 11.84STAKK23 pKa = 8.47TRR25 pKa = 11.84PYY27 pKa = 10.02RR28 pKa = 11.84KK29 pKa = 9.32RR30 pKa = 11.84RR31 pKa = 11.84VTPRR35 pKa = 11.84RR36 pKa = 11.84LTKK39 pKa = 10.37KK40 pKa = 10.23AILNITSKK48 pKa = 10.7KK49 pKa = 9.74KK50 pKa = 10.0RR51 pKa = 11.84NTMLSVTNTNSAGVPVPIALGGVFINGTGSGKK83 pKa = 10.38IIWSPTAMDD92 pKa = 5.59LSTDD96 pKa = 4.68DD97 pKa = 4.31IDD99 pKa = 3.71NTAISSSLRR108 pKa = 11.84TSQVCYY114 pKa = 8.69MRR116 pKa = 11.84GVAEE120 pKa = 4.39HH121 pKa = 6.72LRR123 pKa = 11.84IQTSNGLAWFHH134 pKa = 6.55RR135 pKa = 11.84RR136 pKa = 11.84ICFTSKK142 pKa = 10.61APEE145 pKa = 3.94FRR147 pKa = 11.84YY148 pKa = 9.04MAGDD152 pKa = 3.84SAPNAQWNPYY162 pKa = 9.12IEE164 pKa = 4.48NSNGLQRR171 pKa = 11.84FMLNMQVNNMNNTISRR187 pKa = 11.84MEE189 pKa = 3.84TLIFKK194 pKa = 8.93GQQGKK199 pKa = 9.53DD200 pKa = 2.81WSDD203 pKa = 3.5PLIAPLDD210 pKa = 3.89RR211 pKa = 11.84YY212 pKa = 10.66QIDD215 pKa = 3.57VKK217 pKa = 10.79FDD219 pKa = 3.38KK220 pKa = 10.38TWTIRR225 pKa = 11.84SGNEE229 pKa = 3.2KK230 pKa = 9.36GTVAEE235 pKa = 4.21RR236 pKa = 11.84KK237 pKa = 9.0LWHH240 pKa = 6.76PMNKK244 pKa = 9.53NLVYY248 pKa = 10.77ADD250 pKa = 4.77DD251 pKa = 4.19EE252 pKa = 4.71NGTDD256 pKa = 3.31EE257 pKa = 4.48VTSHH261 pKa = 7.05FSVTDD266 pKa = 3.55KK267 pKa = 11.33QGMGDD272 pKa = 3.62YY273 pKa = 10.36YY274 pKa = 10.19IYY276 pKa = 10.54DD277 pKa = 3.43IFIPGAGSSSGDD289 pKa = 3.72YY290 pKa = 10.98INITPSTTIFWHH302 pKa = 6.17EE303 pKa = 4.0KK304 pKa = 9.48

Molecular weight: 34.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

877

245

328

292.3

32.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.526 ± 0.872

2.166 ± 0.75

7.526 ± 0.864

4.333 ± 0.65

4.333 ± 0.489

9.464 ± 1.417

2.737 ± 0.517

6.157 ± 0.501

4.789 ± 0.92

5.473 ± 0.149

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.052 ± 0.728

3.877 ± 1.501

5.473 ± 0.735

1.938 ± 0.463

8.438 ± 0.096

6.043 ± 0.982

5.359 ± 1.429

5.701 ± 0.754

2.737 ± 0.289

3.877 ± 0.079
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski