Corynebacterium phage Juicebox

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Juiceboxvirus; Corynebacterium virus Juicebox

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385UF80|A0A385UF80_9CAUD Immunity repressor OS=Corynebacterium phage Juicebox OX=2301600 GN=34 PE=4 SV=1
MM1 pKa = 7.66FDD3 pKa = 4.5FIRR6 pKa = 11.84SVVPAGARR14 pKa = 11.84AAWYY18 pKa = 7.14ATAGAVVTALVAWGILDD35 pKa = 3.84DD36 pKa = 4.06TATPAVVGVATAAITLLFAIVHH58 pKa = 6.05SDD60 pKa = 3.46SPVRR64 pKa = 11.84QAGYY68 pKa = 10.72GLAAAVGVLGVYY80 pKa = 9.96LGWGTEE86 pKa = 3.92AQIDD90 pKa = 3.93SLLAVIAPALGLGTAAATTTIGDD113 pKa = 3.47AAYY116 pKa = 10.35VGEE119 pKa = 4.59HH120 pKa = 6.11RR121 pKa = 11.84LEE123 pKa = 4.1NN124 pKa = 3.76

Molecular weight: 12.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385UEU2|A0A385UEU2_9CAUD Uncharacterized protein OS=Corynebacterium phage Juicebox OX=2301600 GN=6 PE=4 SV=1
MM1 pKa = 8.03RR2 pKa = 11.84STCEE6 pKa = 3.93TCGEE10 pKa = 4.14TLPIQRR16 pKa = 11.84AGRR19 pKa = 11.84PPKK22 pKa = 10.32YY23 pKa = 9.75CSSPCRR29 pKa = 11.84QAGYY33 pKa = 9.51RR34 pKa = 11.84ARR36 pKa = 11.84KK37 pKa = 9.07RR38 pKa = 11.84RR39 pKa = 11.84QLPPEE44 pKa = 3.99MVAADD49 pKa = 3.36RR50 pKa = 11.84WVAAEE55 pKa = 4.62GKK57 pKa = 10.27RR58 pKa = 11.84PMTADD63 pKa = 2.84GRR65 pKa = 11.84AASSTDD71 pKa = 2.99PSTWGPRR78 pKa = 11.84AGVADD83 pKa = 4.26GPNGFMLGAGFACIDD98 pKa = 3.68LDD100 pKa = 3.83GCVGVDD106 pKa = 3.66GRR108 pKa = 11.84VEE110 pKa = 3.79PWARR114 pKa = 11.84AILATVPDD122 pKa = 4.14AFVEE126 pKa = 4.38RR127 pKa = 11.84SVSGRR132 pKa = 11.84GLHH135 pKa = 6.28VFGLLPEE142 pKa = 4.76GPGRR146 pKa = 11.84NLGRR150 pKa = 11.84AEE152 pKa = 4.36YY153 pKa = 9.36YY154 pKa = 10.16SKK156 pKa = 11.19SRR158 pKa = 11.84FIRR161 pKa = 11.84VTGNVHH167 pKa = 5.25RR168 pKa = 11.84QGVLVALSPAIAEE181 pKa = 4.04IHH183 pKa = 5.81RR184 pKa = 11.84QHH186 pKa = 7.06AAGQIPQRR194 pKa = 11.84RR195 pKa = 11.84GAARR199 pKa = 11.84RR200 pKa = 11.84RR201 pKa = 11.84AAAAAA206 pKa = 3.78

Molecular weight: 22.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

13163

39

1863

223.1

24.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.953 ± 0.63

0.676 ± 0.146

6.784 ± 0.227

6.533 ± 0.372

2.454 ± 0.165

8.281 ± 0.637

2.051 ± 0.209

4.277 ± 0.227

3.13 ± 0.215

7.931 ± 0.284

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.507 ± 0.133

2.492 ± 0.186

6.328 ± 0.395

3.297 ± 0.232

8.228 ± 0.556

4.87 ± 0.324

6.366 ± 0.275

6.83 ± 0.285

1.99 ± 0.216

2.021 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski