Klebsiella phage ST11-VIM1phi8.2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482J747|A0A482J747_9CAUD ParA-like protein OS=Klebsiella phage ST11-VIM1phi8.2 OX=2555911 PE=4 SV=1
MM1 pKa = 7.94TIRR4 pKa = 11.84FYY6 pKa = 10.61PSRR9 pKa = 11.84LPGEE13 pKa = 4.03PLEE16 pKa = 4.29THH18 pKa = 5.09EE19 pKa = 5.4HH20 pKa = 5.91GVTSIRR26 pKa = 11.84KK27 pKa = 7.67WLVANVEE34 pKa = 4.39GYY36 pKa = 9.82EE37 pKa = 4.18DD38 pKa = 4.67RR39 pKa = 11.84DD40 pKa = 3.91VPPLTVEE47 pKa = 4.17VEE49 pKa = 4.27GLLIPPGEE57 pKa = 3.92WAKK60 pKa = 10.88CVIRR64 pKa = 11.84PDD66 pKa = 3.34SDD68 pKa = 3.21VRR70 pKa = 11.84LYY72 pKa = 9.71PVPFGLEE79 pKa = 3.51AATIAWIGVGISVAAAAYY97 pKa = 10.08SYY99 pKa = 11.76FMMSNIDD106 pKa = 3.32TGGYY110 pKa = 7.72TSSTGRR116 pKa = 11.84SLDD119 pKa = 3.85LNPAKK124 pKa = 10.84ANTAKK129 pKa = 10.79LGDD132 pKa = 4.4AIRR135 pKa = 11.84EE136 pKa = 3.98VFGRR140 pKa = 11.84VRR142 pKa = 11.84IYY144 pKa = 10.21PDD146 pKa = 3.47YY147 pKa = 10.72VVQPVTRR154 pKa = 11.84FDD156 pKa = 3.6AADD159 pKa = 3.58PTKK162 pKa = 10.46MRR164 pKa = 11.84VQMLLCLGVGDD175 pKa = 5.83LIYY178 pKa = 10.63TNGDD182 pKa = 2.7IRR184 pKa = 11.84VGSTPASTLPGFSSTHH200 pKa = 5.3YY201 pKa = 10.41PPGADD206 pKa = 2.83VSGDD210 pKa = 3.34EE211 pKa = 5.5RR212 pKa = 11.84SEE214 pKa = 3.65NWVNSTEE221 pKa = 4.14VGGTSSGTGLDD232 pKa = 3.72MAQTSPDD239 pKa = 3.77ADD241 pKa = 4.73DD242 pKa = 5.4IIADD246 pKa = 3.75SMTVSGSSVTFTGLDD261 pKa = 3.42TDD263 pKa = 5.62DD264 pKa = 6.56DD265 pKa = 4.87DD266 pKa = 7.47DD267 pKa = 5.91NDD269 pKa = 4.01EE270 pKa = 4.96NDD272 pKa = 3.52NALPPSWVAGAVVEE286 pKa = 4.79LKK288 pKa = 10.93APANYY293 pKa = 10.15QITTAAGYY301 pKa = 9.91SVIASPLLTEE311 pKa = 4.5IAPVVGMPVTLGFNSVDD328 pKa = 3.22YY329 pKa = 11.24DD330 pKa = 4.15LFIASYY336 pKa = 9.69TPGQAAVPGTGGSAAKK352 pKa = 9.71VQASAAPTTYY362 pKa = 10.86DD363 pKa = 3.49FSTSSSTFTITWKK376 pKa = 10.61GVTYY380 pKa = 9.93PVSLVANYY388 pKa = 10.54VSMSGLLAAITEE400 pKa = 4.33GLTGSGLVAQDD411 pKa = 3.17NGGTVLITEE420 pKa = 4.44SASPFAGGAITSSSLPAAVFGDD442 pKa = 3.66APVYY446 pKa = 10.1TSGTASTGGSPAVTANVTLAYY467 pKa = 10.73NSATGTAFSGMPEE480 pKa = 4.26GVQRR484 pKa = 11.84LSLAHH489 pKa = 7.04RR490 pKa = 11.84GNEE493 pKa = 3.85YY494 pKa = 10.51RR495 pKa = 11.84IVSADD500 pKa = 3.01GTTATVARR508 pKa = 11.84LVSGAVDD515 pKa = 3.71EE516 pKa = 4.59SWPGFTARR524 pKa = 11.84TMIDD528 pKa = 3.46YY529 pKa = 8.18EE530 pKa = 4.29ATGLNDD536 pKa = 3.52TLSWLGPFLVCPEE549 pKa = 4.35NEE551 pKa = 4.06VVDD554 pKa = 3.95AFEE557 pKa = 5.04VNFSFPNGICGFDD570 pKa = 3.23SKK572 pKa = 11.14GKK574 pKa = 9.73KK575 pKa = 9.55RR576 pKa = 11.84IRR578 pKa = 11.84HH579 pKa = 4.88VEE581 pKa = 3.4WEE583 pKa = 3.79IQYY586 pKa = 10.44RR587 pKa = 11.84VYY589 pKa = 11.08GSGSGWVSHH598 pKa = 5.62QGEE601 pKa = 4.38YY602 pKa = 10.89ALKK605 pKa = 9.96NVNGLGFTEE614 pKa = 5.93RR615 pKa = 11.84ITLSSPGLVEE625 pKa = 3.87VRR627 pKa = 11.84CRR629 pKa = 11.84RR630 pKa = 11.84RR631 pKa = 11.84NEE633 pKa = 3.79QGSNNARR640 pKa = 11.84DD641 pKa = 3.61SMYY644 pKa = 9.71WQALRR649 pKa = 11.84GRR651 pKa = 11.84LLTRR655 pKa = 11.84PSSYY659 pKa = 10.6PGVSLMAVTVEE670 pKa = 4.3TGGKK674 pKa = 9.22LAAQSDD680 pKa = 3.73RR681 pKa = 11.84RR682 pKa = 11.84VNVVATRR689 pKa = 11.84AYY691 pKa = 10.67DD692 pKa = 3.19SGTARR697 pKa = 11.84TISGALLHH705 pKa = 6.04VANSLGLEE713 pKa = 4.01MDD715 pKa = 3.24VDD717 pKa = 4.66TINALEE723 pKa = 4.09SAYY726 pKa = 6.94WTPRR730 pKa = 11.84GEE732 pKa = 4.31NFDD735 pKa = 3.99FATGDD740 pKa = 3.79SISALEE746 pKa = 4.11MLQKK750 pKa = 10.08IANAGKK756 pKa = 10.28SRR758 pKa = 11.84FLLSDD763 pKa = 3.51GLATVNRR770 pKa = 11.84EE771 pKa = 4.13GIKK774 pKa = 10.0PWTGVITPHH783 pKa = 6.1EE784 pKa = 4.38MVEE787 pKa = 4.11EE788 pKa = 4.13LQSGFTVPSDD798 pKa = 3.69DD799 pKa = 5.46DD800 pKa = 4.43FDD802 pKa = 5.26GVDD805 pKa = 3.17VTYY808 pKa = 10.92INGTTWAEE816 pKa = 3.93EE817 pKa = 4.16TVKK820 pKa = 10.51CRR822 pKa = 11.84TPDD825 pKa = 2.88NPTPVKK831 pKa = 10.28IEE833 pKa = 3.85NYY835 pKa = 10.1KK836 pKa = 10.56LDD838 pKa = 3.65GVLTQDD844 pKa = 2.92HH845 pKa = 7.35AYY847 pKa = 9.43QIGMRR852 pKa = 11.84RR853 pKa = 11.84LMKK856 pKa = 10.65YY857 pKa = 10.14LLQRR861 pKa = 11.84VTFQTTTEE869 pKa = 4.14LDD871 pKa = 3.51ALCYY875 pKa = 8.91NTGDD879 pKa = 4.35RR880 pKa = 11.84IVLTDD885 pKa = 5.8DD886 pKa = 3.24IPGNNTISCLVEE898 pKa = 3.99AMTTAGGVTTFTVTEE913 pKa = 4.01PLDD916 pKa = 3.4WSFEE920 pKa = 4.07NPRR923 pKa = 11.84ALIRR927 pKa = 11.84YY928 pKa = 8.54QDD930 pKa = 3.71GSASGLMVASRR941 pKa = 11.84VGDD944 pKa = 3.66FQLSVPHH951 pKa = 7.11LSEE954 pKa = 5.04FDD956 pKa = 4.36DD957 pKa = 3.86PTKK960 pKa = 10.91VDD962 pKa = 4.16LSSATIEE969 pKa = 4.29PIRR972 pKa = 11.84LVFCGSTRR980 pKa = 11.84HH981 pKa = 5.68VYY983 pKa = 10.15DD984 pKa = 5.57AIVEE988 pKa = 4.53EE989 pKa = 4.87IAPQSDD995 pKa = 3.74GTCQVTAKK1003 pKa = 10.17EE1004 pKa = 4.15YY1005 pKa = 11.23LEE1007 pKa = 5.3SFYY1010 pKa = 11.0QYY1012 pKa = 11.67DD1013 pKa = 3.65DD1014 pKa = 3.0ATYY1017 pKa = 10.17PGDD1020 pKa = 3.5AAA1022 pKa = 4.93

Molecular weight: 109.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482J393|A0A482J393_9CAUD CII family protein OS=Klebsiella phage ST11-VIM1phi8.2 OX=2555911 PE=4 SV=1
MM1 pKa = 7.89RR2 pKa = 11.84KK3 pKa = 8.26PARR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 9.32CAHH11 pKa = 5.64CRR13 pKa = 11.84EE14 pKa = 4.41WFHH17 pKa = 6.84PARR20 pKa = 11.84EE21 pKa = 4.26GQVVCSFEE29 pKa = 4.16CASEE33 pKa = 3.93IGKK36 pKa = 8.97KK37 pKa = 7.03QTAKK41 pKa = 10.56ARR43 pKa = 11.84EE44 pKa = 3.93AAKK47 pKa = 10.47ARR49 pKa = 11.84AVKK52 pKa = 10.09RR53 pKa = 11.84QRR55 pKa = 11.84EE56 pKa = 4.09FEE58 pKa = 3.97KK59 pKa = 10.49EE60 pKa = 3.49GRR62 pKa = 11.84QRR64 pKa = 11.84RR65 pKa = 11.84RR66 pKa = 11.84AKK68 pKa = 10.5RR69 pKa = 11.84EE70 pKa = 3.81SFKK73 pKa = 10.93TKK75 pKa = 10.43AQWDD79 pKa = 4.06KK80 pKa = 10.73EE81 pKa = 4.19AQSAFNRR88 pKa = 11.84YY89 pKa = 8.22IRR91 pKa = 11.84IRR93 pKa = 11.84DD94 pKa = 3.38EE95 pKa = 4.24GKK97 pKa = 9.48PCVSCGNPLIGKK109 pKa = 9.17SNYY112 pKa = 8.55LTGSAIDD119 pKa = 3.57ASHH122 pKa = 5.58YY123 pKa = 9.46RR124 pKa = 11.84SRR126 pKa = 11.84GAASHH131 pKa = 6.67LKK133 pKa = 9.12FNVFNVHH140 pKa = 6.17SACTRR145 pKa = 11.84CNRR148 pKa = 11.84QLSGNAVEE156 pKa = 4.7YY157 pKa = 10.2RR158 pKa = 11.84IHH160 pKa = 7.37LIEE163 pKa = 5.58RR164 pKa = 11.84IGLDD168 pKa = 2.75RR169 pKa = 11.84VEE171 pKa = 5.12RR172 pKa = 11.84LEE174 pKa = 4.33ADD176 pKa = 3.23NEE178 pKa = 3.95LRR180 pKa = 11.84RR181 pKa = 11.84FDD183 pKa = 3.47IPYY186 pKa = 9.14LQRR189 pKa = 11.84IKK191 pKa = 11.0SIFTRR196 pKa = 11.84RR197 pKa = 11.84ARR199 pKa = 11.84ALEE202 pKa = 3.86KK203 pKa = 10.4RR204 pKa = 11.84RR205 pKa = 11.84ARR207 pKa = 11.84HH208 pKa = 5.15QEE210 pKa = 3.52AAA212 pKa = 3.66

Molecular weight: 24.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

13865

36

1022

203.9

22.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.657 ± 0.37

1.118 ± 0.133

5.691 ± 0.212

5.604 ± 0.276

3.599 ± 0.257

7.169 ± 0.334

1.608 ± 0.146

5.979 ± 0.259

5.193 ± 0.412

9.203 ± 0.386

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.431 ± 0.139

3.924 ± 0.236

4.183 ± 0.247

4.342 ± 0.442

6.066 ± 0.322

6.542 ± 0.244

6.268 ± 0.438

6.686 ± 0.286

1.717 ± 0.145

3.022 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski