Black currant nucleorhabdovirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Nucleorhabdovirus; unclassified Nucleorhabdovirus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9YRH6|A0A2K9YRH6_9RHAB Putative cell-to-cell movement protein OS=Black currant nucleorhabdovirus 1 OX=2079521 PE=4 SV=1
MM1 pKa = 7.48NIAGGIANVHH11 pKa = 5.92GPYY14 pKa = 10.14SGLPQSALNSEE25 pKa = 4.57VLTSRR30 pKa = 11.84LANEE34 pKa = 4.34VVKK37 pKa = 10.91SDD39 pKa = 4.06TIDD42 pKa = 3.66DD43 pKa = 3.98EE44 pKa = 4.56EE45 pKa = 4.13RR46 pKa = 11.84TIAYY50 pKa = 7.91MKK52 pKa = 10.45EE53 pKa = 3.63WMKK56 pKa = 10.87TMSDD60 pKa = 3.12KK61 pKa = 11.25GINIPTRR68 pKa = 11.84VIEE71 pKa = 4.11VLAEE75 pKa = 3.66ISIVLEE81 pKa = 3.81NSGMDD86 pKa = 3.74DD87 pKa = 3.29LHH89 pKa = 7.59GKK91 pKa = 10.09FGFAMVEE98 pKa = 4.41FGTHH102 pKa = 5.6LMKK105 pKa = 11.0NQIAPNTLLEE115 pKa = 4.34KK116 pKa = 9.01MTGLVDD122 pKa = 3.6KK123 pKa = 10.64LYY125 pKa = 11.09EE126 pKa = 4.48GVNEE130 pKa = 4.12MKK132 pKa = 9.55KK133 pKa = 9.03TNDD136 pKa = 4.97KK137 pKa = 10.76INQDD141 pKa = 3.94LPRR144 pKa = 11.84TIAKK148 pKa = 10.04AKK150 pKa = 9.44RR151 pKa = 11.84QQSGKK156 pKa = 10.16AKK158 pKa = 10.4ASEE161 pKa = 4.07TSPEE165 pKa = 3.92KK166 pKa = 11.03VNNNNLSPPSTAQSNTDD183 pKa = 3.22TGVKK187 pKa = 8.81EE188 pKa = 4.22QEE190 pKa = 4.13EE191 pKa = 4.41KK192 pKa = 10.54MEE194 pKa = 4.26TDD196 pKa = 4.26APALNPKK203 pKa = 9.09SEE205 pKa = 4.17KK206 pKa = 10.52AKK208 pKa = 9.9QLRR211 pKa = 11.84DD212 pKa = 3.39TYY214 pKa = 9.78KK215 pKa = 10.72AHH217 pKa = 6.64YY218 pKa = 7.12STPGFDD224 pKa = 3.76AYY226 pKa = 9.84TPEE229 pKa = 4.33KK230 pKa = 10.41VRR232 pKa = 11.84EE233 pKa = 4.32LITYY237 pKa = 6.85YY238 pKa = 10.65ARR240 pKa = 11.84HH241 pKa = 6.32ILDD244 pKa = 3.55IRR246 pKa = 11.84PDD248 pKa = 2.94IWQDD252 pKa = 3.29DD253 pKa = 3.64ASTFNMVSDD262 pKa = 5.54LIDD265 pKa = 3.57KK266 pKa = 10.46KK267 pKa = 11.01RR268 pKa = 11.84LFIVLSKK275 pKa = 10.88AKK277 pKa = 10.72DD278 pKa = 3.2MGLSYY283 pKa = 10.43EE284 pKa = 4.24DD285 pKa = 3.98HH286 pKa = 5.68ATARR290 pKa = 11.84EE291 pKa = 3.93EE292 pKa = 4.89FIDD295 pKa = 5.55AINTCSPAYY304 pKa = 10.1GSYY307 pKa = 8.98TVQGMIHH314 pKa = 6.98GDD316 pKa = 3.83DD317 pKa = 3.34VWIEE321 pKa = 4.06LIKK324 pKa = 10.79LDD326 pKa = 4.17

Molecular weight: 36.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9YRI2|A0A2K9YRI2_9RHAB Matrix protein OS=Black currant nucleorhabdovirus 1 OX=2079521 PE=4 SV=1
MM1 pKa = 7.38TSSKK5 pKa = 9.51TRR7 pKa = 11.84FSEE10 pKa = 4.17EE11 pKa = 4.19FPSKK15 pKa = 7.42PTKK18 pKa = 9.16YY19 pKa = 9.46VSYY22 pKa = 9.75VAVPIRR28 pKa = 11.84GDD30 pKa = 3.18HH31 pKa = 5.56TEE33 pKa = 4.15IPVSADD39 pKa = 2.79GFLLSTKK46 pKa = 10.35AILYY50 pKa = 9.56IKK52 pKa = 10.37NLLSLSSLEE61 pKa = 3.95VDD63 pKa = 3.86VKK65 pKa = 10.75QVSLAWCPLVEE76 pKa = 4.52PNHH79 pKa = 6.22LSSVTIKK86 pKa = 10.52VNYY89 pKa = 9.89DD90 pKa = 3.01IAHH93 pKa = 6.44GSMLQEE99 pKa = 4.55KK100 pKa = 9.97DD101 pKa = 3.32LSLVRR106 pKa = 11.84LTGKK110 pKa = 10.04ISEE113 pKa = 3.99QHH115 pKa = 6.65GIVLYY120 pKa = 6.98PTRR123 pKa = 11.84PLLYY127 pKa = 9.72PKK129 pKa = 10.24GGKK132 pKa = 8.94LRR134 pKa = 11.84LPWDD138 pKa = 3.62VQVEE142 pKa = 3.93IDD144 pKa = 4.01GVSEE148 pKa = 4.08GSNVDD153 pKa = 3.27QTLGIMKK160 pKa = 8.33VWCQIKK166 pKa = 8.85TKK168 pKa = 10.2PFLSSNTYY176 pKa = 9.7PMKK179 pKa = 10.46LYY181 pKa = 10.22KK182 pKa = 10.51APEE185 pKa = 4.09IKK187 pKa = 9.36WGNVHH192 pKa = 6.62FPFYY196 pKa = 10.36VPYY199 pKa = 10.7YY200 pKa = 9.49IINNVRR206 pKa = 11.84GKK208 pKa = 9.22TSMEE212 pKa = 3.87WEE214 pKa = 3.54NSYY217 pKa = 10.83QYY219 pKa = 11.59EE220 pKa = 4.46LFTKK224 pKa = 10.39VVLDD228 pKa = 3.74HH229 pKa = 6.82VEE231 pKa = 4.02KK232 pKa = 11.02KK233 pKa = 10.78LINQEE238 pKa = 3.56NTLALMQTMSFIDD251 pKa = 3.52CTSINEE257 pKa = 4.1LTKK260 pKa = 11.17NCVLNKK266 pKa = 9.94NGEE269 pKa = 4.17YY270 pKa = 10.01CSCGNRR276 pKa = 11.84VKK278 pKa = 10.72DD279 pKa = 3.7YY280 pKa = 11.84VEE282 pKa = 4.21ICMKK286 pKa = 10.75DD287 pKa = 3.57RR288 pKa = 11.84DD289 pKa = 4.27DD290 pKa = 4.04MYY292 pKa = 10.76TNHH295 pKa = 6.26SSIYY299 pKa = 10.23FEE301 pKa = 4.18TTNGIKK307 pKa = 10.13KK308 pKa = 10.45GLLKK312 pKa = 10.61SSNGARR318 pKa = 11.84VPEE321 pKa = 4.28FF322 pKa = 3.47

Molecular weight: 36.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

4139

276

2106

689.8

78.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.856 ± 1.132

1.957 ± 0.308

6.185 ± 0.441

5.654 ± 0.357

3.6 ± 0.224

5.025 ± 0.244

2.561 ± 0.414

7.466 ± 0.504

7.345 ± 0.462

9.133 ± 0.802

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.213 ± 0.109

4.349 ± 0.306

4.373 ± 0.748

3.141 ± 0.16

4.421 ± 0.607

9.181 ± 0.658

6.282 ± 0.527

5.146 ± 0.368

1.208 ± 0.2

4.905 ± 0.202
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski