Mycobacterium phage SassyB

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U9WRV0|A0A1U9WRV0_9CAUD Uncharacterized protein OS=Mycobacterium phage SassyB OX=1958910 GN=72 PE=4 SV=1
MM1 pKa = 7.59SGDD4 pKa = 3.12INAEE8 pKa = 3.61GFIRR12 pKa = 11.84YY13 pKa = 9.51GGDD16 pKa = 3.52CTCGAIYY23 pKa = 9.12TYY25 pKa = 10.64GGHH28 pKa = 7.08AEE30 pKa = 4.57PGSFDD35 pKa = 4.3PFCPDD40 pKa = 2.7HH41 pKa = 7.27GEE43 pKa = 4.02AAVVATGEE51 pKa = 4.19EE52 pKa = 4.15AA53 pKa = 3.78

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U9WRV1|A0A1U9WRV1_9CAUD Uncharacterized protein OS=Mycobacterium phage SassyB OX=1958910 GN=66 PE=4 SV=1
MM1 pKa = 7.83LSRR4 pKa = 11.84HH5 pKa = 4.81VKK7 pKa = 10.09ALRR10 pKa = 11.84TAVKK14 pKa = 9.18FYY16 pKa = 11.08RR17 pKa = 11.84MSLVAQRR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84EE27 pKa = 3.75EE28 pKa = 3.46WRR30 pKa = 11.84AKK32 pKa = 10.16HH33 pKa = 6.56GEE35 pKa = 4.07ASWITTTGEE44 pKa = 3.79EE45 pKa = 4.09AA46 pKa = 3.64

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

17477

30

1176

184.0

20.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.705 ± 0.447

1.127 ± 0.149

6.614 ± 0.227

5.819 ± 0.307

2.958 ± 0.205

8.84 ± 0.523

2.232 ± 0.203

4.349 ± 0.19

3.29 ± 0.195

7.25 ± 0.218

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.214 ± 0.14

3.33 ± 0.171

6.294 ± 0.222

3.485 ± 0.204

6.752 ± 0.383

5.836 ± 0.26

6.969 ± 0.266

7.209 ± 0.305

2.312 ± 0.142

2.415 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski