Lactobacillus phage JNU_P7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3BEP7|A0A6M3BEP7_9CAUD Uncharacterized protein OS=Lactobacillus phage JNU_P7 OX=2686385 PE=4 SV=1
MM1 pKa = 7.81FDD3 pKa = 3.94TILNNLNTLQDD14 pKa = 3.4EE15 pKa = 4.53MVQMFKK21 pKa = 10.23QQYY24 pKa = 6.91EE25 pKa = 4.14WGWFGKK31 pKa = 8.57TNQEE35 pKa = 3.91SNLVLRR41 pKa = 11.84GYY43 pKa = 11.39VNTNALTPEE52 pKa = 4.85GYY54 pKa = 10.11KK55 pKa = 10.53EE56 pKa = 4.04ITGEE60 pKa = 4.12DD61 pKa = 3.75YY62 pKa = 11.68NEE64 pKa = 3.9TSLNKK69 pKa = 10.25SS70 pKa = 3.16

Molecular weight: 8.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3BES3|A0A6M3BES3_9CAUD Uncharacterized protein OS=Lactobacillus phage JNU_P7 OX=2686385 PE=4 SV=1
MM1 pKa = 7.94RR2 pKa = 11.84KK3 pKa = 8.89QDD5 pKa = 3.8KK6 pKa = 10.23LRR8 pKa = 11.84KK9 pKa = 8.96KK10 pKa = 7.68QAKK13 pKa = 9.09MNIKK17 pKa = 9.7KK18 pKa = 9.79CQRR21 pKa = 11.84KK22 pKa = 8.5YY23 pKa = 10.57RR24 pKa = 11.84KK25 pKa = 9.22AVQRR29 pKa = 11.84AIASGTASTYY39 pKa = 11.08LNLGILYY46 pKa = 10.13VGLRR50 pKa = 11.84SSVEE54 pKa = 4.07LEE56 pKa = 4.2TVILDD61 pKa = 4.14YY62 pKa = 10.69IAKK65 pKa = 9.39KK66 pKa = 9.79YY67 pKa = 10.44KK68 pKa = 10.21KK69 pKa = 9.99AGSNQRR75 pKa = 11.84RR76 pKa = 11.84LVNGG80 pKa = 3.54

Molecular weight: 9.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11371

35

1694

206.7

23.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.088 ± 0.371

0.44 ± 0.082

6.842 ± 0.248

5.655 ± 0.45

3.861 ± 0.271

6.235 ± 0.556

1.557 ± 0.129

6.402 ± 0.249

9.559 ± 0.417

7.519 ± 0.386

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.454 ± 0.132

6.2 ± 0.322

3.359 ± 0.193

4.696 ± 0.207

4.01 ± 0.394

6.552 ± 0.31

6.024 ± 0.365

5.857 ± 0.208

1.433 ± 0.164

4.256 ± 0.387
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski