Porcine picobirnavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Picobirnaviridae; Picobirnavirus; unclassified Picobirnavirus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6B0R5|W6B0R5_9VIRU Uncharacterized protein OS=Porcine picobirnavirus OX=442302 PE=4 SV=1
MM1 pKa = 7.01ATKK4 pKa = 10.3NKK6 pKa = 9.59KK7 pKa = 10.24FNGKK11 pKa = 8.46GQGYY15 pKa = 9.39RR16 pKa = 11.84RR17 pKa = 11.84GSKK20 pKa = 10.0SRR22 pKa = 11.84SDD24 pKa = 3.79SYY26 pKa = 12.02SKK28 pKa = 10.97DD29 pKa = 3.23KK30 pKa = 11.48SDD32 pKa = 3.68FSYY35 pKa = 11.4EE36 pKa = 4.08EE37 pKa = 4.03EE38 pKa = 4.36TKK40 pKa = 10.69SGKK43 pKa = 9.5DD44 pKa = 3.1RR45 pKa = 11.84QKK47 pKa = 11.21CNSSRR52 pKa = 11.84PNDD55 pKa = 3.89WKK57 pKa = 10.52WYY59 pKa = 8.27AQNEE63 pKa = 4.11QLLRR67 pKa = 11.84DD68 pKa = 3.95SASFSYY74 pKa = 8.41NTPLGTVVRR83 pKa = 11.84RR84 pKa = 11.84TVSGDD89 pKa = 3.34QPVDD93 pKa = 3.78FAGVPGVMAIYY104 pKa = 7.5TTPAYY109 pKa = 10.12GNSDD113 pKa = 3.65SAVSPLNVAIRR124 pKa = 11.84NIYY127 pKa = 10.34SFVRR131 pKa = 11.84HH132 pKa = 6.08ANSGHH137 pKa = 6.44ANYY140 pKa = 8.96DD141 pKa = 3.51APDD144 pKa = 3.56LGIYY148 pKa = 9.76LMAMDD153 pKa = 5.54NIYY156 pKa = 11.13SFMAYY161 pKa = 9.89LRR163 pKa = 11.84RR164 pKa = 11.84IYY166 pKa = 10.03GVAMTYY172 pKa = 10.49SYY174 pKa = 10.52TNKK177 pKa = 9.6YY178 pKa = 9.68YY179 pKa = 10.24PVAVTNAMGVDD190 pKa = 4.55FEE192 pKa = 5.29DD193 pKa = 3.33VHH195 pKa = 8.38ARR197 pKa = 11.84LADD200 pKa = 4.14FRR202 pKa = 11.84AMINSFAVKK211 pKa = 9.61VGSMCIPSSMSVFARR226 pKa = 11.84HH227 pKa = 4.03MWMYY231 pKa = 10.34EE232 pKa = 3.84GYY234 pKa = 8.33YY235 pKa = 10.23TDD237 pKa = 3.4TDD239 pKa = 3.53QNKK242 pKa = 8.91AQTYY246 pKa = 9.14LYY248 pKa = 10.22VPTGFYY254 pKa = 10.19HH255 pKa = 5.76YY256 pKa = 10.58RR257 pKa = 11.84LRR259 pKa = 11.84NKK261 pKa = 10.03LVEE264 pKa = 4.24EE265 pKa = 4.49GVYY268 pKa = 9.24DD269 pKa = 3.59TSGMLWYY276 pKa = 10.38KK277 pKa = 10.6PLTPYY282 pKa = 10.7GNPVSQEE289 pKa = 3.72VSQVNLLTTEE299 pKa = 4.07MLHH302 pKa = 6.59QYY304 pKa = 9.82GEE306 pKa = 4.28AMLEE310 pKa = 3.91PVLRR314 pKa = 11.84SEE316 pKa = 5.68DD317 pKa = 3.78MNIMSGDD324 pKa = 3.25ILKK327 pKa = 10.81AFGSNGIYY335 pKa = 8.53MIPMIPEE342 pKa = 3.99NYY344 pKa = 9.37VVVPSYY350 pKa = 11.05SRR352 pKa = 11.84EE353 pKa = 3.9VLDD356 pKa = 5.18QINNCTLIGDD366 pKa = 3.97NVHH369 pKa = 5.47YY370 pKa = 9.95TEE372 pKa = 5.26EE373 pKa = 4.54ADD375 pKa = 3.59FDD377 pKa = 3.51KK378 pKa = 11.34GYY380 pKa = 11.05NVLVQDD386 pKa = 4.55PKK388 pKa = 10.81HH389 pKa = 6.46GYY391 pKa = 10.32LIGKK395 pKa = 6.4TCTEE399 pKa = 3.64IPMGVAEE406 pKa = 5.02KK407 pKa = 9.73LTLNMDD413 pKa = 4.09RR414 pKa = 11.84LTAKK418 pKa = 9.74TFVNFDD424 pKa = 3.77HH425 pKa = 7.98DD426 pKa = 4.52DD427 pKa = 3.59VTPADD432 pKa = 3.69TMVATRR438 pKa = 11.84LTNVYY443 pKa = 9.87DD444 pKa = 4.15PKK446 pKa = 10.82DD447 pKa = 3.49VSIIARR453 pKa = 11.84GIGGGAAIQIRR464 pKa = 11.84PGDD467 pKa = 3.65KK468 pKa = 10.72SITLVNTTGTDD479 pKa = 2.8IANYY483 pKa = 10.44ARR485 pKa = 11.84IFYY488 pKa = 8.8YY489 pKa = 10.95ANGGLIVSEE498 pKa = 4.72MICALLPVTLQWDD511 pKa = 4.62FTTMTTEE518 pKa = 3.6KK519 pKa = 10.72LLRR522 pKa = 11.84EE523 pKa = 4.33YY524 pKa = 10.8VSKK527 pKa = 10.94AVTSMQLDD535 pKa = 3.81TARR538 pKa = 11.84INMVTNFRR546 pKa = 11.84RR547 pKa = 11.84HH548 pKa = 5.19PRR550 pKa = 11.84IAYY553 pKa = 9.36QYY555 pKa = 9.29SLYY558 pKa = 11.13VKK560 pKa = 9.25ATADD564 pKa = 3.67GAPKK568 pKa = 9.99EE569 pKa = 4.34VNEE572 pKa = 4.14HH573 pKa = 5.13GKK575 pKa = 10.17YY576 pKa = 9.94VADD579 pKa = 5.02ISDD582 pKa = 3.98LNQWTVIGSQEE593 pKa = 4.32LCNMAEE599 pKa = 4.06TALLSEE605 pKa = 4.95FNVTQYY611 pKa = 10.76GRR613 pKa = 11.84KK614 pKa = 9.26AA615 pKa = 3.14

Molecular weight: 69.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6B0S5|W6B0S5_9VIRU RNA-dependent RNA polymerase OS=Porcine picobirnavirus OX=442302 PE=4 SV=1
MM1 pKa = 7.87PKK3 pKa = 10.47NNANKK8 pKa = 10.2RR9 pKa = 11.84SRR11 pKa = 11.84DD12 pKa = 3.55YY13 pKa = 10.54FASSFASSANLRR25 pKa = 11.84AYY27 pKa = 10.02FGSVVKK33 pKa = 10.49GQSEE37 pKa = 5.11VYY39 pKa = 8.01DD40 pKa = 3.67TPFARR45 pKa = 11.84DD46 pKa = 3.35EE47 pKa = 4.27STKK50 pKa = 10.55TLLSKK55 pKa = 10.1WKK57 pKa = 10.22AVLKK61 pKa = 10.43SIEE64 pKa = 4.24NTWPSLYY71 pKa = 10.32KK72 pKa = 10.58YY73 pKa = 10.37EE74 pKa = 4.76IDD76 pKa = 3.57LASKK80 pKa = 9.51VGPMSVRR87 pKa = 11.84KK88 pKa = 8.68PLSEE92 pKa = 3.78RR93 pKa = 11.84MEE95 pKa = 5.37DD96 pKa = 3.3IEE98 pKa = 5.88HH99 pKa = 6.59YY100 pKa = 10.38YY101 pKa = 11.34ADD103 pKa = 3.84ILQSAEE109 pKa = 4.16PLSDD113 pKa = 3.06RR114 pKa = 11.84AIAATIRR121 pKa = 11.84EE122 pKa = 4.28FNRR125 pKa = 11.84LRR127 pKa = 11.84GLRR130 pKa = 11.84LRR132 pKa = 11.84SEE134 pKa = 4.13SRR136 pKa = 11.84TVEE139 pKa = 3.94LMKK142 pKa = 10.75KK143 pKa = 8.14STNSGSPYY151 pKa = 7.45FTKK154 pKa = 10.47RR155 pKa = 11.84RR156 pKa = 11.84LVTDD160 pKa = 3.12KK161 pKa = 10.34TVPSEE166 pKa = 4.31VYY168 pKa = 9.67HH169 pKa = 6.56FRR171 pKa = 11.84DD172 pKa = 3.37EE173 pKa = 4.57VEE175 pKa = 4.02QHH177 pKa = 6.09LSSGDD182 pKa = 3.38FTAAAILGWRR192 pKa = 11.84GQEE195 pKa = 4.13GGPTAADD202 pKa = 3.35VKK204 pKa = 10.98QRR206 pKa = 11.84VVWMFPYY213 pKa = 10.4AVNIAEE219 pKa = 4.25LQLYY223 pKa = 9.45QPFIEE228 pKa = 4.83AAQSSISSQLGLALTRR244 pKa = 11.84LINGSLPCSTQRR256 pKa = 11.84QTNDD260 pKa = 3.22LVVCTDD266 pKa = 3.76FSKK269 pKa = 10.9FDD271 pKa = 3.21QHH273 pKa = 7.11FNAEE277 pKa = 4.07MQAAALRR284 pKa = 11.84ILRR287 pKa = 11.84ACMEE291 pKa = 4.39PSMTSTNWLNDD302 pKa = 3.41VYY304 pKa = 9.95PIKK307 pKa = 11.05YY308 pKa = 7.97MIPLAYY314 pKa = 10.08DD315 pKa = 3.12EE316 pKa = 4.66GKK318 pKa = 10.52VRR320 pKa = 11.84FGKK323 pKa = 9.95HH324 pKa = 4.43GMGSGSGGTNCDD336 pKa = 2.62EE337 pKa = 4.46TLAHH341 pKa = 7.44RR342 pKa = 11.84ALQYY346 pKa = 10.24EE347 pKa = 4.71AAQSSGVKK355 pKa = 10.17LNPNSQCLGDD365 pKa = 5.2DD366 pKa = 3.95GVLTYY371 pKa = 10.34PGITVEE377 pKa = 4.98DD378 pKa = 3.74VVKK381 pKa = 10.39AYY383 pKa = 9.76KK384 pKa = 10.32SHH386 pKa = 5.73GLEE389 pKa = 3.97MNLDD393 pKa = 3.4KK394 pKa = 11.05QYY396 pKa = 11.78ASTQDD401 pKa = 3.2CTYY404 pKa = 10.58LRR406 pKa = 11.84RR407 pKa = 11.84WHH409 pKa = 6.68HH410 pKa = 6.13KK411 pKa = 10.17DD412 pKa = 3.14YY413 pKa = 10.73RR414 pKa = 11.84QDD416 pKa = 4.15GICVGVYY423 pKa = 6.78STCRR427 pKa = 11.84ALGRR431 pKa = 11.84LRR433 pKa = 11.84YY434 pKa = 9.64LEE436 pKa = 5.54RR437 pKa = 11.84YY438 pKa = 8.61QNPKK442 pKa = 9.36YY443 pKa = 9.89WDD445 pKa = 3.4AKK447 pKa = 10.45AVALRR452 pKa = 11.84QLSILEE458 pKa = 4.01NVKK461 pKa = 9.79YY462 pKa = 10.47HH463 pKa = 6.1PLKK466 pKa = 10.33EE467 pKa = 4.09QFVDD471 pKa = 4.52FCMKK475 pKa = 9.73RR476 pKa = 11.84DD477 pKa = 3.6KK478 pKa = 11.17YY479 pKa = 11.06RR480 pKa = 11.84LGLDD484 pKa = 3.01IPHH487 pKa = 6.82FFDD490 pKa = 4.36DD491 pKa = 4.24LRR493 pKa = 11.84AITEE497 pKa = 3.98QKK499 pKa = 9.99IDD501 pKa = 4.34DD502 pKa = 4.07MPDD505 pKa = 2.97FLGYY509 pKa = 9.01TRR511 pKa = 11.84TLQSGGDD518 pKa = 3.53PAGGIEE524 pKa = 3.96NWWIVKK530 pKa = 8.25YY531 pKa = 10.64LKK533 pKa = 10.78SKK535 pKa = 10.67

Molecular weight: 60.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1328

178

615

442.7

49.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.605 ± 0.086

1.13 ± 0.34

5.949 ± 0.599

5.723 ± 1.335

3.012 ± 0.458

6.551 ± 0.232

2.108 ± 0.309

4.593 ± 0.299

5.045 ± 0.897

7.605 ± 0.865

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.313 ± 0.682

5.12 ± 0.785

3.614 ± 0.709

4.292 ± 0.957

6.325 ± 1.079

7.831 ± 0.571

6.627 ± 0.773

6.702 ± 0.717

1.28 ± 0.227

5.572 ± 1.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski