Deinococcus gobiensis (strain DSM 21396 / JCM 16679 / CGMCC 1.7299 / I-0)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Deinococcus-Thermus; Deinococci; Deinococcales; Deinococcaceae; Deinococcus; Deinococcus gobiensis

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4329 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H8GTE9|H8GTE9_DEIGI Transcriptional regulator LysR family OS=Deinococcus gobiensis (strain DSM 21396 / JCM 16679 / CGMCC 1.7299 / I-0) OX=745776 GN=oxyR PE=3 SV=1
MM1 pKa = 7.72DD2 pKa = 3.2VWLSQCRR9 pKa = 11.84AQVCDD14 pKa = 3.46RR15 pKa = 11.84VSAGLSWEE23 pKa = 4.63AGDD26 pKa = 5.05EE27 pKa = 4.13PDD29 pKa = 6.04LEE31 pKa = 5.98LLNEE35 pKa = 4.18WNGNYY40 pKa = 7.55YY41 pKa = 8.08TQAYY45 pKa = 9.57VYY47 pKa = 10.4DD48 pKa = 4.41DD49 pKa = 4.59SYY51 pKa = 11.97HH52 pKa = 7.0LDD54 pKa = 2.67SSMILRR60 pKa = 11.84GGYY63 pKa = 7.64TRR65 pKa = 11.84AALVRR70 pKa = 11.84WMEE73 pKa = 4.23LLLEE77 pKa = 4.9DD78 pKa = 4.9GGDD81 pKa = 3.91FEE83 pKa = 6.85AEE85 pKa = 4.06LGG87 pKa = 3.77

Molecular weight: 9.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H8GS49|H8GS49_DEIGI Uncharacterized protein OS=Deinococcus gobiensis (strain DSM 21396 / JCM 16679 / CGMCC 1.7299 / I-0) OX=745776 GN=DGo_CA0017 PE=4 SV=1
MM1 pKa = 7.21SRR3 pKa = 11.84SRR5 pKa = 11.84TPRR8 pKa = 11.84RR9 pKa = 11.84PPPRR13 pKa = 11.84APSARR18 pKa = 11.84FTATGRR24 pKa = 11.84PKK26 pKa = 10.32RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84HH30 pKa = 4.43AFGWFSVALRR40 pKa = 11.84IGLVLLLLLWGLILWHH56 pKa = 6.87RR57 pKa = 11.84LGG59 pKa = 4.13

Molecular weight: 6.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4329

0

4329

1234809

8

3354

285.2

30.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.848 ± 0.065

0.595 ± 0.011

5.109 ± 0.03

5.506 ± 0.042

3.011 ± 0.027

9.437 ± 0.046

2.076 ± 0.022

3.215 ± 0.032

2.347 ± 0.035

11.699 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.838 ± 0.018

2.302 ± 0.033

5.95 ± 0.042

3.919 ± 0.032

7.683 ± 0.044

4.941 ± 0.037

5.987 ± 0.042

7.768 ± 0.033

1.409 ± 0.016

2.36 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski