Mycobacterium phage Wanda

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Omegavirus; unclassified Omegavirus

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 240 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5XW26|S5XW26_9CAUD Terminase OS=Mycobacterium phage Wanda OX=1340713 GN=10 PE=4 SV=1
MM1 pKa = 7.15HH2 pKa = 7.02QPTEE6 pKa = 4.72RR7 pKa = 11.84ITPMTVFDD15 pKa = 4.93ANHH18 pKa = 5.73NEE20 pKa = 3.53YY21 pKa = 10.7RR22 pKa = 11.84IGDD25 pKa = 3.9KK26 pKa = 10.79VGSPVSIDD34 pKa = 2.92RR35 pKa = 11.84GMVDD39 pKa = 4.39LYY41 pKa = 10.67TVTEE45 pKa = 3.89ILEE48 pKa = 4.23ADD50 pKa = 3.76SYY52 pKa = 9.91GNEE55 pKa = 3.92YY56 pKa = 10.54VRR58 pKa = 11.84VEE60 pKa = 4.46FIGDD64 pKa = 3.25EE65 pKa = 4.32GSRR68 pKa = 11.84EE69 pKa = 4.11TEE71 pKa = 3.35LWAAEE76 pKa = 3.96DD77 pKa = 3.37LAIYY81 pKa = 10.15VGADD85 pKa = 3.5GYY87 pKa = 11.26SIEE90 pKa = 5.1LNVNEE95 pKa = 4.79AGYY98 pKa = 11.02AGLTIASSDD107 pKa = 3.57RR108 pKa = 11.84ILFEE112 pKa = 4.43MNDD115 pKa = 3.68CFASEE120 pKa = 3.77AAEE123 pKa = 3.99FVTFYY128 pKa = 11.34LEE130 pKa = 4.48TIEE133 pKa = 4.48SS134 pKa = 3.69

Molecular weight: 14.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A068LLN5|A0A068LLN5_9CAUD Uncharacterized protein OS=Mycobacterium phage Wanda OX=1340713 GN=191 PE=4 SV=1
MM1 pKa = 7.03NTTPNRR7 pKa = 11.84PTITDD12 pKa = 3.32VCADD16 pKa = 3.58LHH18 pKa = 5.85DD19 pKa = 5.08AGWRR23 pKa = 11.84TVDD26 pKa = 2.8TRR28 pKa = 11.84YY29 pKa = 10.52RR30 pKa = 11.84GTQAVIEE37 pKa = 4.27FARR40 pKa = 11.84RR41 pKa = 11.84GRR43 pKa = 11.84KK44 pKa = 8.02VWALVDD50 pKa = 3.38TRR52 pKa = 11.84TNDD55 pKa = 3.52VIGGTAVRR63 pKa = 11.84EE64 pKa = 3.79IRR66 pKa = 11.84AAEE69 pKa = 3.88MVRR72 pKa = 11.84EE73 pKa = 3.91SEE75 pKa = 3.86YY76 pKa = 10.44RR77 pKa = 11.84KK78 pKa = 9.59RR79 pKa = 11.84VRR81 pKa = 11.84AYY83 pKa = 9.01RR84 pKa = 11.84TVDD87 pKa = 2.65ADD89 pKa = 3.47ARR91 pKa = 11.84KK92 pKa = 8.45GTDD95 pKa = 2.64WEE97 pKa = 4.35RR98 pKa = 11.84RR99 pKa = 3.65

Molecular weight: 11.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

240

0

240

34503

22

1643

143.8

15.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.672 ± 0.225

1.498 ± 0.148

6.397 ± 0.159

6.895 ± 0.241

3.243 ± 0.107

8.759 ± 0.351

2.264 ± 0.105

4.829 ± 0.117

3.994 ± 0.173

7.973 ± 0.187

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.443 ± 0.101

3.333 ± 0.126

5.446 ± 0.143

3.142 ± 0.15

6.739 ± 0.264

5.495 ± 0.165

5.669 ± 0.199

6.904 ± 0.214

2.29 ± 0.112

3.017 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski