Sewage-associated circular DNA virus-31

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UGZ8|A0A0B4UGZ8_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-31 OX=1592098 PE=4 SV=1
MM1 pKa = 7.71PARR4 pKa = 11.84LEE6 pKa = 3.99IEE8 pKa = 4.53SYY10 pKa = 10.73SSYY13 pKa = 11.05LIYY16 pKa = 11.03LNLFLSIYY24 pKa = 8.95FLKK27 pKa = 10.94YY28 pKa = 7.92QTNLMAYY35 pKa = 8.95KK36 pKa = 10.68DD37 pKa = 3.91SANTANKK44 pKa = 10.07YY45 pKa = 10.32FLLLGDD51 pKa = 5.31RR52 pKa = 11.84ILDD55 pKa = 3.79CVEE58 pKa = 4.41LNPDD62 pKa = 4.26GVCEE66 pKa = 4.2ADD68 pKa = 4.56DD69 pKa = 3.57VQLFINRR76 pKa = 11.84SSIFLSDD83 pKa = 3.55VGLKK87 pKa = 8.78AHH89 pKa = 6.61KK90 pKa = 9.28WVIGQLHH97 pKa = 7.19HH98 pKa = 6.95YY99 pKa = 9.31NNEE102 pKa = 3.94YY103 pKa = 9.75TKK105 pKa = 11.24GRR107 pKa = 11.84MSEE110 pKa = 4.11HH111 pKa = 6.57EE112 pKa = 4.12LVEE115 pKa = 4.36AYY117 pKa = 10.26GYY119 pKa = 11.32LNLEE123 pKa = 4.17GYY125 pKa = 9.22LRR127 pKa = 11.84HH128 pKa = 5.85VAYY131 pKa = 7.6EE132 pKa = 4.09TYY134 pKa = 10.66KK135 pKa = 10.26EE136 pKa = 4.16VRR138 pKa = 11.84GKK140 pKa = 9.52WEE142 pKa = 4.07TRR144 pKa = 11.84VVEE147 pKa = 4.07YY148 pKa = 10.69DD149 pKa = 3.41LKK151 pKa = 11.49GDD153 pKa = 3.77VASVNYY159 pKa = 9.78CLRR162 pKa = 11.84RR163 pKa = 11.84LEE165 pKa = 4.21LMAIARR171 pKa = 11.84SQGTTLKK178 pKa = 10.78QIFKK182 pKa = 9.72SNGWEE187 pKa = 4.46GYY189 pKa = 10.18LDD191 pKa = 4.54RR192 pKa = 11.84EE193 pKa = 4.36YY194 pKa = 11.22QPIEE198 pKa = 3.95YY199 pKa = 10.35QFVDD203 pKa = 4.73PYY205 pKa = 11.6LLDD208 pKa = 3.58KK209 pKa = 10.78FGRR212 pKa = 11.84EE213 pKa = 3.83AWEE216 pKa = 4.1YY217 pKa = 10.54TPDD220 pKa = 3.7PYY222 pKa = 10.68ATHH225 pKa = 6.91KK226 pKa = 11.03GSLL229 pKa = 3.55

Molecular weight: 26.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UGN7|A0A0B4UGN7_9VIRU Uncharacterized protein OS=Sewage-associated circular DNA virus-31 OX=1592098 PE=4 SV=1
MM1 pKa = 7.02YY2 pKa = 10.59GKK4 pKa = 10.43FRR6 pKa = 11.84GSKK9 pKa = 8.75GASSRR14 pKa = 11.84RR15 pKa = 11.84TNGITANNIARR26 pKa = 11.84ALKK29 pKa = 10.35KK30 pKa = 10.84VGGKK34 pKa = 7.84YY35 pKa = 8.26TSKK38 pKa = 10.53KK39 pKa = 9.05YY40 pKa = 9.72SAPRR44 pKa = 11.84FATVGFARR52 pKa = 11.84NVEE55 pKa = 3.97KK56 pKa = 10.62KK57 pKa = 10.6YY58 pKa = 10.77FDD60 pKa = 3.2KK61 pKa = 11.11QYY63 pKa = 10.94ISNSLEE69 pKa = 3.73ALTGITGNSASNGVTYY85 pKa = 10.62ISNVWGSYY93 pKa = 9.84NFGAQVASTAISNDD107 pKa = 2.82MCKK110 pKa = 10.48GLDD113 pKa = 3.2TGTTARR119 pKa = 11.84TRR121 pKa = 11.84IGNKK125 pKa = 9.97LKK127 pKa = 10.68VSYY130 pKa = 10.45IKK132 pKa = 10.9GAFTFTAAQSNGMNNTPQNGEE153 pKa = 4.0NQVVPLAANTKK164 pKa = 8.97QDD166 pKa = 3.87YY167 pKa = 11.12LRR169 pKa = 11.84TTYY172 pKa = 10.81RR173 pKa = 11.84FVIVKK178 pKa = 9.64DD179 pKa = 3.6LQVNSTDD186 pKa = 4.11AQVTWAQVFDD196 pKa = 4.07TTGSQAGVHH205 pKa = 6.45AEE207 pKa = 4.0LNVDD211 pKa = 2.87NMGRR215 pKa = 11.84FIVIQDD221 pKa = 3.41KK222 pKa = 11.18YY223 pKa = 10.28FTLDD227 pKa = 3.16AQNPQQTCPFQVKK240 pKa = 9.91GSSVGNVRR248 pKa = 11.84YY249 pKa = 9.87NGPGAAALTDD259 pKa = 3.24KK260 pKa = 11.09GIYY263 pKa = 8.44VVWAAYY269 pKa = 10.43VMGVDD274 pKa = 4.37GVGTANVQCPSPVGHH289 pKa = 6.58SRR291 pKa = 11.84LCFTDD296 pKa = 3.9DD297 pKa = 3.11

Molecular weight: 32.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

924

229

398

308.0

34.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.359 ± 0.979

1.84 ± 0.352

5.087 ± 0.205

6.277 ± 2.007

3.896 ± 0.2

8.658 ± 0.861

1.84 ± 0.473

4.437 ± 0.192

6.818 ± 0.526

6.385 ± 2.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.056 ± 0.24

5.736 ± 0.956

3.03 ± 0.255

4.437 ± 0.493

5.087 ± 0.299

5.303 ± 0.865

6.602 ± 1.109

7.143 ± 0.799

2.381 ± 0.749

5.628 ± 1.313
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski