Mycobacterium phage Heffalump

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A220NSC7|A0A220NSC7_9CAUD Capsid maturation protease OS=Mycobacterium phage Heffalump OX=1983575 GN=16 PE=4 SV=1
MM1 pKa = 8.05ADD3 pKa = 4.05LSIPPIPYY11 pKa = 10.39ALRR14 pKa = 11.84EE15 pKa = 3.98YY16 pKa = 9.13LTEE19 pKa = 4.01YY20 pKa = 10.65EE21 pKa = 4.22RR22 pKa = 11.84LRR24 pKa = 11.84NADD27 pKa = 3.99CEE29 pKa = 4.32DD30 pKa = 3.87TNLLLGLADD39 pKa = 4.48SIAIEE44 pKa = 4.21VRR46 pKa = 11.84ALGFPDD52 pKa = 4.2LPYY55 pKa = 10.88DD56 pKa = 4.32AGLGPSNN63 pKa = 4.08

Molecular weight: 6.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A220NSB1|A0A220NSB1_9CAUD Uncharacterized protein OS=Mycobacterium phage Heffalump OX=1983575 GN=2 PE=4 SV=1
MM1 pKa = 7.29SWASSRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84YY10 pKa = 9.77DD11 pKa = 4.66LPPDD15 pKa = 3.14WEE17 pKa = 4.41LNYY20 pKa = 10.26RR21 pKa = 11.84LPVLRR26 pKa = 11.84DD27 pKa = 3.59ANWICEE33 pKa = 4.27LQWNGCVGVASEE45 pKa = 4.24VDD47 pKa = 3.59HH48 pKa = 6.9IKK50 pKa = 10.61RR51 pKa = 11.84GNDD54 pKa = 3.11HH55 pKa = 6.61SRR57 pKa = 11.84SNLQAVCHH65 pKa = 6.16RR66 pKa = 11.84CHH68 pKa = 6.56AKK70 pKa = 10.26KK71 pKa = 10.66SSAEE75 pKa = 3.83GNARR79 pKa = 11.84KK80 pKa = 10.1AEE82 pKa = 3.73LRR84 pKa = 11.84ARR86 pKa = 11.84RR87 pKa = 11.84KK88 pKa = 10.31RR89 pKa = 11.84PDD91 pKa = 2.97EE92 pKa = 3.82RR93 pKa = 11.84HH94 pKa = 5.48PGRR97 pKa = 11.84RR98 pKa = 3.44

Molecular weight: 11.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

16557

38

847

180.0

19.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.96 ± 0.368

0.93 ± 0.118

6.372 ± 0.25

6.843 ± 0.347

3.237 ± 0.174

8.601 ± 0.529

1.933 ± 0.163

4.922 ± 0.16

4.651 ± 0.201

8.208 ± 0.369

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.398 ± 0.118

3.261 ± 0.16

5.544 ± 0.268

3.551 ± 0.254

6.354 ± 0.368

5.267 ± 0.235

6.136 ± 0.222

7.018 ± 0.238

1.927 ± 0.154

2.887 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski