Haloarcula hispanica icosahedral virus 2

Taxonomy: Viruses; Varidnaviria; Helvetiavirae; Dividoviricota; Laserviricetes; Halopanivirales; Sphaerolipoviridae; Alphasphaerolipovirus

Average proteome isoelectric point is 4.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9AZV9|H9AZV9_9VIRU Uncharacterized protein OS=Haloarcula hispanica icosahedral virus 2 OX=1154689 PE=4 SV=1
MM1 pKa = 7.73SRR3 pKa = 11.84GDD5 pKa = 3.77PLGDD9 pKa = 3.6PPEE12 pKa = 4.3EE13 pKa = 4.82DD14 pKa = 4.28NLFTDD19 pKa = 3.81TDD21 pKa = 3.97DD22 pKa = 4.93AAGQPGPSSDD32 pKa = 4.42PGPLPPTSGPVEE44 pKa = 4.11AALSTLTYY52 pKa = 10.89AEE54 pKa = 4.76GSAFGLGICPVLTALLVLPYY74 pKa = 10.58DD75 pKa = 3.77PTLSLVALASAGLLTASTAARR96 pKa = 11.84SALPLGHH103 pKa = 6.51VGANAHH109 pKa = 5.79YY110 pKa = 10.64LFAGQLAGAVLGSLWVALVTLAAAVGGVVAA140 pKa = 5.55

Molecular weight: 13.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9AZX1|H9AZX1_9VIRU VP7 OS=Haloarcula hispanica icosahedral virus 2 OX=1154689 PE=1 SV=1
MM1 pKa = 7.82ASINVNRR8 pKa = 11.84ATSTLMSTSFLKK20 pKa = 10.24SAVMIVVGSLLAQVVTDD37 pKa = 3.6YY38 pKa = 11.22LRR40 pKa = 11.84SNVRR44 pKa = 11.84DD45 pKa = 3.3INMAGGDD52 pKa = 3.63AVYY55 pKa = 10.68SAVAALLVLTVLPKK69 pKa = 10.57KK70 pKa = 10.23YY71 pKa = 10.39GKK73 pKa = 9.33PLALGSMATSVRR85 pKa = 11.84VMLRR89 pKa = 11.84EE90 pKa = 4.03LGVVV94 pKa = 3.46

Molecular weight: 9.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

9343

60

1777

217.3

23.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.81 ± 0.506

0.835 ± 0.225

10.628 ± 0.513

8.648 ± 0.576

1.969 ± 0.165

9.419 ± 0.46

1.948 ± 0.235

2.729 ± 0.207

1.948 ± 0.238

6.732 ± 0.423

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.798 ± 0.207

2.847 ± 0.227

4.923 ± 0.303

3.414 ± 0.255

6.186 ± 0.39

6.037 ± 0.342

6.336 ± 0.398

8.306 ± 0.394

1.327 ± 0.151

3.157 ± 0.29
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski