Paucibacter sp. KCTC 42545

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Paucibacter; unclassified Paucibacter

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4190 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U3DUW0|A0A0U3DUW0_9BURK Nitrate reductase OS=Paucibacter sp. KCTC 42545 OX=1768242 GN=AT984_21615 PE=4 SV=1
MM1 pKa = 7.59CLICGWIYY9 pKa = 11.2DD10 pKa = 4.08EE11 pKa = 4.96AAGDD15 pKa = 4.19PEE17 pKa = 5.37HH18 pKa = 7.07GIAPGTLWADD28 pKa = 2.81VDD30 pKa = 4.43MNWTCPEE37 pKa = 3.86CGARR41 pKa = 11.84KK42 pKa = 9.87DD43 pKa = 3.6DD44 pKa = 3.99FEE46 pKa = 4.37MVRR49 pKa = 11.84II50 pKa = 4.15

Molecular weight: 5.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U3D7B9|A0A0U3D7B9_9BURK Uncharacterized protein OS=Paucibacter sp. KCTC 42545 OX=1768242 GN=AT984_00960 PE=4 SV=1
MM1 pKa = 7.35RR2 pKa = 11.84HH3 pKa = 5.03RR4 pKa = 11.84VIARR8 pKa = 11.84GHH10 pKa = 4.87IHH12 pKa = 5.55QAKK15 pKa = 8.72ATSKK19 pKa = 10.03NQPLHH24 pKa = 5.99SATSKK29 pKa = 8.57VQRR32 pKa = 11.84PAFFAALKK40 pKa = 10.15GGRR43 pKa = 11.84RR44 pKa = 11.84PQIGRR49 pKa = 11.84SKK51 pKa = 9.42KK52 pKa = 8.63QSRR55 pKa = 11.84ATPAFQQIKK64 pKa = 10.07PIDD67 pKa = 3.78KK68 pKa = 10.68QPP70 pKa = 3.06

Molecular weight: 7.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4190

0

4190

1461542

50

6269

348.8

37.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.184 ± 0.055

0.893 ± 0.013

4.938 ± 0.025

5.284 ± 0.037

3.439 ± 0.028

8.191 ± 0.049

2.077 ± 0.02

4.117 ± 0.027

3.467 ± 0.032

11.666 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.392 ± 0.02

2.767 ± 0.034

4.975 ± 0.036

4.816 ± 0.039

6.441 ± 0.043

6.102 ± 0.049

4.674 ± 0.033

6.985 ± 0.031

1.499 ± 0.017

2.093 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski