Cronobacter phage ENT39118

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F1C579|F1C579_9CAUD Uncharacterized protein OS=Cronobacter phage ENT39118 OX=984175 PE=4 SV=1
MM1 pKa = 7.56TEE3 pKa = 4.08DD4 pKa = 3.43NLYY7 pKa = 10.3PLLAHH12 pKa = 7.17LAGGQVYY19 pKa = 8.98PYY21 pKa = 9.85VAPLGSDD28 pKa = 4.08GKK30 pKa = 10.04PSVSPPWVIYY40 pKa = 10.26SIITDD45 pKa = 3.62VAADD49 pKa = 3.76VLCGQAEE56 pKa = 4.49SAVSVQVDD64 pKa = 4.3VYY66 pKa = 11.4SSTITEE72 pKa = 3.76ARR74 pKa = 11.84TIRR77 pKa = 11.84DD78 pKa = 3.42MALDD82 pKa = 4.15ALQALKK88 pKa = 10.6PEE90 pKa = 4.99NIVKK94 pKa = 8.96TPSYY98 pKa = 10.67EE99 pKa = 3.68PDD101 pKa = 3.26LRR103 pKa = 11.84YY104 pKa = 9.87HH105 pKa = 6.93RR106 pKa = 11.84ATLEE110 pKa = 4.02FQVTVV115 pKa = 3.48

Molecular weight: 12.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F1C581|F1C581_9CAUD Head-tail connector protein OS=Cronobacter phage ENT39118 OX=984175 PE=4 SV=1
MM1 pKa = 7.2EE2 pKa = 6.02PGRR5 pKa = 11.84FRR7 pKa = 11.84HH8 pKa = 5.45RR9 pKa = 11.84VKK11 pKa = 10.54ILTFTTSRR19 pKa = 11.84DD20 pKa = 3.36PSGQPVEE27 pKa = 4.07WWSGGNPVPAEE38 pKa = 3.94VKK40 pKa = 10.32GISGRR45 pKa = 11.84EE46 pKa = 3.87QLSGGAEE53 pKa = 3.94TAQATIRR60 pKa = 11.84VWMRR64 pKa = 11.84FRR66 pKa = 11.84SEE68 pKa = 4.39LNASSRR74 pKa = 11.84LEE76 pKa = 4.17VLSGPYY82 pKa = 9.45KK83 pKa = 10.57GQVLNIIGPPVANATGSRR101 pKa = 11.84LEE103 pKa = 4.11ILCKK107 pKa = 10.23TGVEE111 pKa = 4.41KK112 pKa = 10.98

Molecular weight: 12.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

38

0

38

10939

108

1090

287.9

31.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.495 ± 0.62

1.079 ± 0.187

5.732 ± 0.238

5.869 ± 0.337

3.492 ± 0.211

7.953 ± 0.343

1.828 ± 0.305

5.293 ± 0.239

4.882 ± 0.324

8.319 ± 0.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.285 ± 0.136

4.251 ± 0.252

4.077 ± 0.327

4.562 ± 0.305

6.235 ± 0.272

6.317 ± 0.442

6.07 ± 0.32

6.646 ± 0.273

1.645 ± 0.128

2.971 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski