Stenomitos frigidus ULC18

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Leptolyngbyaceae; Stenomitos; Stenomitos frigidus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5916 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T1ED57|A0A2T1ED57_9CYAN Radical SAM protein OS=Stenomitos frigidus ULC18 OX=2107698 GN=C7B82_08340 PE=4 SV=1
MM1 pKa = 7.51NEE3 pKa = 4.27HH4 pKa = 6.75IALPLCKK11 pKa = 9.84GLSMPVKK18 pKa = 10.24NFRR21 pKa = 11.84HH22 pKa = 4.68VRR24 pKa = 11.84KK25 pKa = 8.57ATVVKK30 pKa = 9.71TPDD33 pKa = 2.99GGQIKK38 pKa = 10.08LGSYY42 pKa = 10.34RR43 pKa = 11.84PDD45 pKa = 3.08KK46 pKa = 10.97KK47 pKa = 10.94NPNDD51 pKa = 3.42KK52 pKa = 9.64TYY54 pKa = 10.84KK55 pKa = 9.92SSRR58 pKa = 11.84STDD61 pKa = 3.61DD62 pKa = 4.18LPSKK66 pKa = 10.93VDD68 pKa = 3.41LRR70 pKa = 11.84PYY72 pKa = 6.64MTPVEE77 pKa = 4.14NQGNSNSCTANAMAGAYY94 pKa = 8.02EE95 pKa = 4.09YY96 pKa = 10.69LAKK99 pKa = 10.13RR100 pKa = 11.84VKK102 pKa = 10.71GSAHH106 pKa = 5.94DD107 pKa = 3.64VSRR110 pKa = 11.84LFIYY114 pKa = 10.89YY115 pKa = 9.9NARR118 pKa = 11.84EE119 pKa = 4.16LDD121 pKa = 3.47GATDD125 pKa = 3.79SDD127 pKa = 3.53EE128 pKa = 4.09GTYY131 pKa = 10.55LRR133 pKa = 11.84SCVKK137 pKa = 10.19VLKK140 pKa = 10.57KK141 pKa = 10.86YY142 pKa = 7.35GTCAEE147 pKa = 4.46TTWPFDD153 pKa = 2.94LHH155 pKa = 7.64RR156 pKa = 11.84IFEE159 pKa = 4.66PPHH162 pKa = 5.49EE163 pKa = 4.38SAYY166 pKa = 10.0TEE168 pKa = 3.9ATSFLIEE175 pKa = 3.62DD176 pKa = 4.48AYY178 pKa = 10.63RR179 pKa = 11.84VKK181 pKa = 10.88VDD183 pKa = 4.19LDD185 pKa = 3.47AMRR188 pKa = 11.84QCLADD193 pKa = 4.63GYY195 pKa = 9.62PFTFGLDD202 pKa = 3.46LFSSFQKK209 pKa = 10.63AGSKK213 pKa = 10.7GLVPMPDD220 pKa = 3.73PDD222 pKa = 4.73SEE224 pKa = 4.31QHH226 pKa = 6.87DD227 pKa = 4.14GGHH230 pKa = 7.12AMLCVGYY237 pKa = 10.66SDD239 pKa = 3.9ADD241 pKa = 3.36KK242 pKa = 11.46VFIVRR247 pKa = 11.84NSWGEE252 pKa = 3.51GWGDD256 pKa = 3.24HH257 pKa = 6.45GYY259 pKa = 11.03CYY261 pKa = 10.18IPYY264 pKa = 10.19DD265 pKa = 4.07YY266 pKa = 7.67MTNPDD271 pKa = 3.96LNGDD275 pKa = 3.87LWAIRR280 pKa = 11.84HH281 pKa = 5.35VADD284 pKa = 4.6ADD286 pKa = 3.45IDD288 pKa = 4.09LSQDD292 pKa = 2.87IQGEE296 pKa = 4.37RR297 pKa = 11.84TSLFSGATAAIAHH310 pKa = 6.68AMQLPRR316 pKa = 11.84ASRR319 pKa = 11.84TTEE322 pKa = 3.44YY323 pKa = 11.35DD324 pKa = 2.66NDD326 pKa = 3.82YY327 pKa = 8.97TVEE330 pKa = 3.99YY331 pKa = 9.63AAQSVFVNGGFIAISDD347 pKa = 4.11YY348 pKa = 11.62EE349 pKa = 4.59DD350 pKa = 4.75LDD352 pKa = 3.65SLYY355 pKa = 10.43FAEE358 pKa = 5.51YY359 pKa = 9.16EE360 pKa = 4.04DD361 pKa = 4.62EE362 pKa = 4.5YY363 pKa = 11.5EE364 pKa = 4.28EE365 pKa = 4.23FTLTEE370 pKa = 3.99TSEE373 pKa = 4.15EE374 pKa = 4.2TEE376 pKa = 3.93YY377 pKa = 11.03SYY379 pKa = 11.86EE380 pKa = 4.08EE381 pKa = 4.15TSEE384 pKa = 4.12EE385 pKa = 4.07TSEE388 pKa = 4.03EE389 pKa = 4.1TEE391 pKa = 3.91YY392 pKa = 11.03SYY394 pKa = 11.84EE395 pKa = 4.09EE396 pKa = 3.85TSEE399 pKa = 3.94EE400 pKa = 4.23SEE402 pKa = 4.07EE403 pKa = 4.35SEE405 pKa = 4.24EE406 pKa = 4.41TEE408 pKa = 4.07DD409 pKa = 3.95SYY411 pKa = 12.0EE412 pKa = 4.09EE413 pKa = 3.93EE414 pKa = 4.44SEE416 pKa = 4.24EE417 pKa = 4.61EE418 pKa = 3.94EE419 pKa = 5.51SEE421 pKa = 5.12DD422 pKa = 3.85EE423 pKa = 4.55DD424 pKa = 5.27EE425 pKa = 4.6EE426 pKa = 5.59SEE428 pKa = 5.23DD429 pKa = 3.71EE430 pKa = 4.19NEE432 pKa = 4.08EE433 pKa = 3.96DD434 pKa = 4.22SEE436 pKa = 4.53EE437 pKa = 4.55IEE439 pKa = 4.88DD440 pKa = 4.14SYY442 pKa = 12.12EE443 pKa = 3.87EE444 pKa = 4.71DD445 pKa = 3.94SEE447 pKa = 5.05DD448 pKa = 4.76DD449 pKa = 3.59EE450 pKa = 5.93SEE452 pKa = 4.41EE453 pKa = 4.04EE454 pKa = 4.17DD455 pKa = 4.25SEE457 pKa = 5.0EE458 pKa = 4.01EE459 pKa = 4.24DD460 pKa = 3.81SEE462 pKa = 4.15EE463 pKa = 4.42DD464 pKa = 3.67EE465 pKa = 4.42EE466 pKa = 5.6SEE468 pKa = 4.71EE469 pKa = 4.11EE470 pKa = 4.28DD471 pKa = 4.15SEE473 pKa = 4.34EE474 pKa = 4.33TEE476 pKa = 4.49DD477 pKa = 4.79SYY479 pKa = 12.2EE480 pKa = 4.03EE481 pKa = 4.51DD482 pKa = 3.6SEE484 pKa = 4.27EE485 pKa = 4.74DD486 pKa = 3.34EE487 pKa = 5.18SEE489 pKa = 4.39EE490 pKa = 3.93EE491 pKa = 4.07DD492 pKa = 3.86SEE494 pKa = 4.61EE495 pKa = 4.28EE496 pKa = 3.92EE497 pKa = 4.8SEE499 pKa = 4.25AEE501 pKa = 3.96PEE503 pKa = 4.14EE504 pKa = 4.39EE505 pKa = 4.61SEE507 pKa = 4.06EE508 pKa = 4.39DD509 pKa = 3.55YY510 pKa = 11.88GDD512 pKa = 4.47AEE514 pKa = 4.01ASYY517 pKa = 11.48DD518 pKa = 3.94EE519 pKa = 4.89GGEE522 pKa = 4.13DD523 pKa = 3.25EE524 pKa = 5.32GGGEE528 pKa = 4.39GEE530 pKa = 4.44EE531 pKa = 4.37EE532 pKa = 4.02

Molecular weight: 60.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T1DZU7|A0A2T1DZU7_9CYAN Tfp pilus assembly protein PilF OS=Stenomitos frigidus ULC18 OX=2107698 GN=C7B82_21165 PE=4 SV=1
MM1 pKa = 7.55TKK3 pKa = 9.01RR4 pKa = 11.84TLGGTSRR11 pKa = 11.84KK12 pKa = 9.47RR13 pKa = 11.84KK14 pKa = 7.16RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TATGRR29 pKa = 11.84RR30 pKa = 11.84VIQARR35 pKa = 11.84RR36 pKa = 11.84TKK38 pKa = 10.62GRR40 pKa = 11.84FRR42 pKa = 11.84LAVAA46 pKa = 4.63

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5916

0

5916

1851076

25

2980

312.9

34.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.776 ± 0.033

0.957 ± 0.01

5.146 ± 0.022

5.649 ± 0.034

3.699 ± 0.021

6.81 ± 0.037

2.059 ± 0.019

5.457 ± 0.023

3.867 ± 0.027

11.448 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.888 ± 0.014

3.36 ± 0.026

5.128 ± 0.026

5.682 ± 0.037

5.736 ± 0.026

6.224 ± 0.029

6.055 ± 0.032

6.872 ± 0.028

1.463 ± 0.015

2.724 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski