Vibrio phage VfO4K68

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Versovirus; Vibrio virus VfO3K6

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9MBX5|Q9MBX5_9VIRU Vpf81 OS=Vibrio phage VfO4K68 OX=127508 GN=vpf81 PE=4 SV=1
MM1 pKa = 7.28NKK3 pKa = 10.06SLFLLLFSCLFLSLNASAAQPTYY26 pKa = 10.54KK27 pKa = 10.5VSDD30 pKa = 3.43VSAYY34 pKa = 9.5PDD36 pKa = 3.59CKK38 pKa = 10.69LLLGLRR44 pKa = 11.84VNPASYY50 pKa = 10.56VSCYY54 pKa = 8.61EE55 pKa = 4.05NKK57 pKa = 9.73FVNYY61 pKa = 9.99KK62 pKa = 10.62DD63 pKa = 4.74FSTKK67 pKa = 9.71SCYY70 pKa = 9.75LRR72 pKa = 11.84HH73 pKa = 5.84GKK75 pKa = 9.97YY76 pKa = 9.97VVDD79 pKa = 4.27IMCHH83 pKa = 4.35TTSPSWPLYY92 pKa = 9.22RR93 pKa = 11.84AAGFLQNSAQCPPDD107 pKa = 3.53YY108 pKa = 11.08EE109 pKa = 4.4KK110 pKa = 11.45VEE112 pKa = 4.15DD113 pKa = 4.48EE114 pKa = 4.63YY115 pKa = 11.89VVSCEE120 pKa = 4.44PIVPACEE127 pKa = 3.92YY128 pKa = 11.15GEE130 pKa = 4.53NPDD133 pKa = 4.02GSCMDD138 pKa = 3.64ACQFKK143 pKa = 10.73QSIGDD148 pKa = 3.7TVKK151 pKa = 10.86LYY153 pKa = 8.08WHH155 pKa = 6.48PAIYY159 pKa = 10.72GEE161 pKa = 4.73LVTGACYY168 pKa = 10.65GDD170 pKa = 4.13YY171 pKa = 10.89GATRR175 pKa = 11.84CEE177 pKa = 4.02VTKK180 pKa = 11.03NEE182 pKa = 4.01STIICTGVPDD192 pKa = 4.14GQYY195 pKa = 10.09TPDD198 pKa = 3.67SQCSLRR204 pKa = 11.84FAYY207 pKa = 9.17TGRR210 pKa = 11.84QCDD213 pKa = 3.3GGTLFWGVNGPDD225 pKa = 3.77EE226 pKa = 5.2PIIPPDD232 pKa = 3.61TPEE235 pKa = 5.51DD236 pKa = 3.9PTHH239 pKa = 7.31DD240 pKa = 4.39PDD242 pKa = 5.96DD243 pKa = 4.23PTDD246 pKa = 4.15EE247 pKa = 5.35IEE249 pKa = 5.27DD250 pKa = 3.98PTVLPDD256 pKa = 4.76DD257 pKa = 3.92STNTVNPGVVDD268 pKa = 4.59DD269 pKa = 5.26KK270 pKa = 11.58PDD272 pKa = 3.54VEE274 pKa = 6.19DD275 pKa = 5.25PDD277 pKa = 4.19TDD279 pKa = 3.44EE280 pKa = 4.48STDD283 pKa = 3.65TAVLSAIKK291 pKa = 10.32GLNVDD296 pKa = 3.91VNKK299 pKa = 10.75GIHH302 pKa = 6.7DD303 pKa = 4.72LNVDD307 pKa = 3.85INQSHH312 pKa = 7.08ADD314 pKa = 3.21ITNAVIDD321 pKa = 4.2VKK323 pKa = 11.2GSLVDD328 pKa = 3.34NTQAIQEE335 pKa = 4.02QQINDD340 pKa = 3.12NKK342 pKa = 10.54IYY344 pKa = 10.9NNTKK348 pKa = 10.74ALIQQANGDD357 pKa = 3.16ITTAVNNNTNATIGIRR373 pKa = 11.84DD374 pKa = 4.0DD375 pKa = 4.27LKK377 pKa = 11.52GLGDD381 pKa = 3.9SVGEE385 pKa = 4.31LDD387 pKa = 4.0SSLNAIEE394 pKa = 4.64GLLTGSEE401 pKa = 4.55FGTPTGTAITGEE413 pKa = 4.12IFTAEE418 pKa = 4.26DD419 pKa = 3.57FANLQTTIDD428 pKa = 3.92EE429 pKa = 4.45KK430 pKa = 11.47AEE432 pKa = 4.29SIQGYY437 pKa = 9.97VDD439 pKa = 4.67DD440 pKa = 4.85IKK442 pKa = 11.59GLITIGTNFNNGTLSDD458 pKa = 3.38KK459 pKa = 11.13SFNIKK464 pKa = 10.03GATVEE469 pKa = 4.33SGLQRR474 pKa = 11.84FDD476 pKa = 3.62AVSGYY481 pKa = 9.57VRR483 pKa = 11.84PVVLFICALIALWVLFGNRR502 pKa = 11.84SKK504 pKa = 11.51

Molecular weight: 54.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9MCC2|Q9MCC2_9VIRU Vpf215 OS=Vibrio phage VfO4K68 OX=127508 GN=vpf215 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.36GLDD5 pKa = 3.31MKK7 pKa = 11.07YY8 pKa = 9.36VFRR11 pKa = 11.84GKK13 pKa = 10.48SYY15 pKa = 7.69TTLTSCYY22 pKa = 9.68RR23 pKa = 11.84DD24 pKa = 3.22NTDD27 pKa = 2.87QVTVGIGTVRR37 pKa = 11.84TRR39 pKa = 11.84LKK41 pKa = 11.02NGWSLNKK48 pKa = 10.24ALLHH52 pKa = 6.2PKK54 pKa = 10.02QKK56 pKa = 9.7TIKK59 pKa = 8.75TKK61 pKa = 10.61LGSHH65 pKa = 4.77TVEE68 pKa = 3.85GKK70 pKa = 10.6VYY72 pKa = 10.6EE73 pKa = 4.2NLPSIAEE80 pKa = 4.22EE81 pKa = 4.11YY82 pKa = 11.43GMTLNTIYY90 pKa = 10.55KK91 pKa = 9.43RR92 pKa = 11.84YY93 pKa = 10.22SRR95 pKa = 11.84GCRR98 pKa = 11.84GDD100 pKa = 4.28DD101 pKa = 3.72LVPLKK106 pKa = 10.5KK107 pKa = 9.97RR108 pKa = 11.84KK109 pKa = 10.15SYY111 pKa = 10.07VAPDD115 pKa = 3.53DD116 pKa = 3.58EE117 pKa = 6.1ANFRR121 pKa = 11.84FYY123 pKa = 11.6ANGVGYY129 pKa = 10.55KK130 pKa = 9.83SAADD134 pKa = 3.47ACRR137 pKa = 11.84KK138 pKa = 10.2LNVKK142 pKa = 10.03YY143 pKa = 8.94GTYY146 pKa = 9.93RR147 pKa = 11.84LHH149 pKa = 6.38MSNGFTVEE157 pKa = 3.77QALGIEE163 pKa = 4.44KK164 pKa = 10.43VQDD167 pKa = 3.24GRR169 pKa = 11.84KK170 pKa = 9.56VRR172 pKa = 11.84GTKK175 pKa = 10.3FNVDD179 pKa = 2.89GKK181 pKa = 10.77EE182 pKa = 3.81YY183 pKa = 10.13TINEE187 pKa = 3.99LSILHH192 pKa = 5.63KK193 pKa = 10.32VVRR196 pKa = 11.84NWSRR200 pKa = 11.84RR201 pKa = 11.84FHH203 pKa = 6.02EE204 pKa = 4.54RR205 pKa = 11.84SSNHH209 pKa = 6.24LLRR212 pKa = 11.84LATT215 pKa = 4.07

Molecular weight: 24.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1972

76

504

246.5

27.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.846 ± 0.716

2.079 ± 0.442

6.389 ± 0.914

5.325 ± 0.454

4.513 ± 0.583

7.606 ± 0.443

2.282 ± 0.48

5.527 ± 0.608

5.477 ± 0.715

8.671 ± 0.428

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.724 ± 0.41

5.02 ± 0.489

4.26 ± 0.632

3.347 ± 0.415

4.564 ± 0.875

6.491 ± 0.426

6.643 ± 0.617

7.657 ± 0.859

1.471 ± 0.281

4.108 ± 0.423
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski