Janthinobacterium sp. B9-8

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Janthinobacterium; unclassified Janthinobacterium

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4080 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A120II39|A0A120II39_9BURK Uncharacterized protein OS=Janthinobacterium sp. B9-8 OX=1236179 GN=VN23_15720 PE=4 SV=1
MM1 pKa = 6.87NTVTDD6 pKa = 3.77MPIPFVFTDD15 pKa = 3.78SAAEE19 pKa = 4.02KK20 pKa = 10.73VRR22 pKa = 11.84DD23 pKa = 4.06LIIEE27 pKa = 4.3EE28 pKa = 4.57GNPDD32 pKa = 3.03LKK34 pKa = 11.14LRR36 pKa = 11.84VFVTGGGCSGFQYY49 pKa = 10.94GFTFDD54 pKa = 4.81EE55 pKa = 4.64ITNEE59 pKa = 4.02DD60 pKa = 3.73DD61 pKa = 4.08TPVEE65 pKa = 4.3KK66 pKa = 11.1NGVTLLVDD74 pKa = 3.69PMSYY78 pKa = 10.34QYY80 pKa = 11.57LVGAEE85 pKa = 3.69IDD87 pKa = 3.85YY88 pKa = 11.28VEE90 pKa = 4.54SLEE93 pKa = 4.67GSQFTIKK100 pKa = 10.58NPNASTTCGCGSSFSVV116 pKa = 3.54

Molecular weight:
12.6 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0Y0USV9|A0A0Y0USV9_9BURK Cobyric acid synthase OS=Janthinobacterium sp. B9-8 OX=1236179 GN=VN23_01805 PE=4 SV=1
MM1 pKa = 7.41VLFIIIFCGFLLFEE15 pKa = 4.77LVLGNLPWWIAGGYY29 pKa = 10.6LLMSTVAFIAYY40 pKa = 10.05ALDD43 pKa = 3.54KK44 pKa = 11.07SAARR48 pKa = 11.84NNRR51 pKa = 11.84WRR53 pKa = 11.84TPEE56 pKa = 3.92RR57 pKa = 11.84TLHH60 pKa = 6.05LLGVLGGWRR69 pKa = 11.84GALLAQRR76 pKa = 11.84VLRR79 pKa = 11.84HH80 pKa = 5.86KK81 pKa = 9.72SAKK84 pKa = 9.84VVFLRR89 pKa = 11.84VFVATIVLNCAALACLLAVGGFWQGG114 pKa = 2.77

Molecular weight:
12.65 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4080

0

4080

1323239

37

4936

324.3

35.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.892 ± 0.061

0.976 ± 0.014

5.14 ± 0.032

5.535 ± 0.046

3.901 ± 0.031

7.221 ± 0.057

2.152 ± 0.022

5.881 ± 0.03

4.769 ± 0.044

11.25 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.529 ± 0.024

3.678 ± 0.036

4.335 ± 0.032

4.529 ± 0.038

5.06 ± 0.045

6.53 ± 0.044

5.001 ± 0.067

6.64 ± 0.041

1.353 ± 0.02

2.628 ± 0.023

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski