Actinomyces sp. CtC 72

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinomyces; unclassified Actinomyces

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2185 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G1PJV4|A0A2G1PJV4_9ACTO ABC transporter OS=Actinomyces sp. CtC 72 OX=1937003 GN=BW737_001935 PE=3 SV=1
MM1 pKa = 7.03TAATSTGPSTEE12 pKa = 5.22DD13 pKa = 3.4LDD15 pKa = 4.65ALNALDD21 pKa = 5.33LRR23 pKa = 11.84TPLASYY29 pKa = 7.65ATPSGVQAGSTPFGQFAAGQVSTPAGAGPYY59 pKa = 9.4TPDD62 pKa = 3.0ASLPYY67 pKa = 10.1SLTWPGMASGTSVVVSGGAYY87 pKa = 9.75GVNTDD92 pKa = 5.1DD93 pKa = 4.97LTDD96 pKa = 4.33LAATLNGAADD106 pKa = 3.76WLEE109 pKa = 4.12DD110 pKa = 3.96ARR112 pKa = 11.84DD113 pKa = 3.51LALTSLTDD121 pKa = 3.52LRR123 pKa = 11.84CNGFGFTMYY132 pKa = 9.77RR133 pKa = 11.84RR134 pKa = 11.84PMTASDD140 pKa = 3.16QHH142 pKa = 5.71NFRR145 pKa = 11.84FGMPSQSSTASDD157 pKa = 3.44QSSSGFISKK166 pKa = 10.27SDD168 pKa = 3.37ALTSGGGQCYY178 pKa = 9.42IWQLDD183 pKa = 3.66PCATEE188 pKa = 3.95CANAISALEE197 pKa = 4.0ALTSGVGSLDD207 pKa = 3.75DD208 pKa = 4.07AATSLRR214 pKa = 11.84TLATDD219 pKa = 3.78ITACGTAYY227 pKa = 9.81TDD229 pKa = 4.25ADD231 pKa = 3.98TEE233 pKa = 4.42IDD235 pKa = 4.16GKK237 pKa = 9.41WLLMLQGLAWDD248 pKa = 3.97RR249 pKa = 11.84TFPAA253 pKa = 4.64

Molecular weight: 26.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G1PJL8|A0A2G1PJL8_9ACTO Gluconate 5-dehydrogenase OS=Actinomyces sp. CtC 72 OX=1937003 GN=BW737_002735 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84HH12 pKa = 5.28RR13 pKa = 11.84AKK15 pKa = 10.38VHH17 pKa = 5.26GFRR20 pKa = 11.84KK21 pKa = 9.9RR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AVLASRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.61GRR40 pKa = 11.84ARR42 pKa = 11.84LAAA45 pKa = 4.44

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2185

0

2185

625708

32

1321

286.4

30.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.451 ± 0.08

0.859 ± 0.014

6.185 ± 0.044

5.437 ± 0.05

2.709 ± 0.038

8.742 ± 0.04

2.026 ± 0.029

4.092 ± 0.043

1.795 ± 0.032

10.034 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.067 ± 0.025

2.145 ± 0.031

5.517 ± 0.044

2.956 ± 0.027

7.376 ± 0.068

5.699 ± 0.044

6.443 ± 0.049

8.628 ± 0.051

1.605 ± 0.024

2.233 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski