Lactococcus phage 936 group phage PhiF0139

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Skunavirus; Lactococcus virus PhiF0139

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126HBF5|A0A126HBF5_9CAUD Neck passage structure OS=Lactococcus phage 936 group phage PhiF0139 OX=1636578 GN=PhiF0139_12 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 10.41VYY4 pKa = 10.54VLSGDD9 pKa = 4.17TYY11 pKa = 9.3CGSWGSEE18 pKa = 3.01ISLFGVFSNKK28 pKa = 9.67EE29 pKa = 3.93EE30 pKa = 4.1AEE32 pKa = 4.07KK33 pKa = 10.81LADD36 pKa = 5.34KK37 pKa = 10.35IRR39 pKa = 11.84CDD41 pKa = 3.32ISIVNIDD48 pKa = 3.61EE49 pKa = 4.32VEE51 pKa = 4.03EE52 pKa = 3.94PKK54 pKa = 11.18YY55 pKa = 10.98LGGYY59 pKa = 9.59CEE61 pKa = 4.07

Molecular weight: 6.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126HBE0|A0A126HBE0_9CAUD Tape measure protein OS=Lactococcus phage 936 group phage PhiF0139 OX=1636578 GN=PhiF0139_16 PE=4 SV=1
MM1 pKa = 7.46CKK3 pKa = 9.75KK4 pKa = 10.38RR5 pKa = 11.84KK6 pKa = 5.78YY7 pKa = 8.56TKK9 pKa = 9.78MGALYY14 pKa = 10.54SIVNAQHH21 pKa = 6.05NKK23 pKa = 9.46KK24 pKa = 10.06KK25 pKa = 10.41ADD27 pKa = 4.21KK28 pKa = 10.12IPVRR32 pKa = 11.84AYY34 pKa = 8.84YY35 pKa = 10.05CKK37 pKa = 9.82WCNLYY42 pKa = 10.42HH43 pKa = 7.32LSSQQRR49 pKa = 11.84LNIKK53 pKa = 9.21TGVIGG58 pKa = 3.97

Molecular weight: 6.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

9279

36

876

162.8

18.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.003 ± 0.588

0.744 ± 0.173

5.83 ± 0.191

7.727 ± 0.528

4.203 ± 0.23

6.003 ± 0.462

1.272 ± 0.169

7.005 ± 0.314

9.322 ± 0.441

8.686 ± 0.356

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.414 ± 0.151

6.66 ± 0.271

2.22 ± 0.234

3.632 ± 0.213

3.449 ± 0.287

6.358 ± 0.504

6.606 ± 0.31

6.208 ± 0.371

1.423 ± 0.13

4.235 ± 0.384
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski