Amino acid dipepetide frequency for Methanobrevibacter woesei

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.077AlaAla: 4.077 ± 0.125
0.934AlaCys: 0.934 ± 0.061
3.646AlaAsp: 3.646 ± 0.093
4.171AlaGlu: 4.171 ± 0.112
2.432AlaPhe: 2.432 ± 0.078
4.043AlaGly: 4.043 ± 0.113
1.025AlaHis: 1.025 ± 0.053
6.681AlaIle: 6.681 ± 0.151
4.4AlaLys: 4.4 ± 0.102
5.29AlaLeu: 5.29 ± 0.12
1.533AlaMet: 1.533 ± 0.062
2.987AlaAsn: 2.987 ± 0.094
1.853AlaPro: 1.853 ± 0.063
1.369AlaGln: 1.369 ± 0.062
2.086AlaArg: 2.086 ± 0.064
3.836AlaSer: 3.836 ± 0.089
3.048AlaThr: 3.048 ± 0.102
4.413AlaVal: 4.413 ± 0.109
0.327AlaTrp: 0.327 ± 0.029
1.896AlaTyr: 1.896 ± 0.066
0.0AlaXaa: 0.0 ± 0.0
Cys
0.701CysAla: 0.701 ± 0.045
0.211CysCys: 0.211 ± 0.025
0.875CysAsp: 0.875 ± 0.043
1.071CysGlu: 1.071 ± 0.049
0.407CysPhe: 0.407 ± 0.033
1.663CysGly: 1.663 ± 0.085
0.255CysHis: 0.255 ± 0.023
0.862CysIle: 0.862 ± 0.054
0.814CysLys: 0.814 ± 0.048
0.886CysLeu: 0.886 ± 0.052
0.285CysMet: 0.285 ± 0.027
0.605CysAsn: 0.605 ± 0.04
0.919CysPro: 0.919 ± 0.074
0.313CysGln: 0.313 ± 0.027
0.414CysArg: 0.414 ± 0.032
0.812CysSer: 0.812 ± 0.044
0.557CysThr: 0.557 ± 0.04
0.74CysVal: 0.74 ± 0.042
0.067CysTrp: 0.067 ± 0.011
0.479CysTyr: 0.479 ± 0.032
0.0CysXaa: 0.0 ± 0.0
Asp
3.727AspAla: 3.727 ± 0.098
0.834AspCys: 0.834 ± 0.043
4.356AspAsp: 4.356 ± 0.117
6.113AspGlu: 6.113 ± 0.112
3.27AspPhe: 3.27 ± 0.078
3.642AspGly: 3.642 ± 0.092
0.749AspHis: 0.749 ± 0.037
6.744AspIle: 6.744 ± 0.133
5.018AspLys: 5.018 ± 0.122
6.276AspLeu: 6.276 ± 0.121
1.689AspMet: 1.689 ± 0.058
3.953AspAsn: 3.953 ± 0.124
1.794AspPro: 1.794 ± 0.073
0.932AspGln: 0.932 ± 0.046
1.596AspArg: 1.596 ± 0.064
3.929AspSer: 3.929 ± 0.105
2.641AspThr: 2.641 ± 0.069
4.933AspVal: 4.933 ± 0.122
0.455AspTrp: 0.455 ± 0.03
3.165AspTyr: 3.165 ± 0.101
0.0AspXaa: 0.0 ± 0.0
Glu
4.421GluAla: 4.421 ± 0.11
0.768GluCys: 0.768 ± 0.044
5.325GluAsp: 5.325 ± 0.101
7.77GluGlu: 7.77 ± 0.172
3.424GluPhe: 3.424 ± 0.097
4.404GluGly: 4.404 ± 0.095
1.167GluHis: 1.167 ± 0.049
8.101GluIle: 8.101 ± 0.157
6.881GluLys: 6.881 ± 0.156
7.334GluLeu: 7.334 ± 0.143
2.029GluMet: 2.029 ± 0.069
5.665GluAsn: 5.665 ± 0.135
1.82GluPro: 1.82 ± 0.064
1.463GluGln: 1.463 ± 0.061
2.434GluArg: 2.434 ± 0.074
4.13GluSer: 4.13 ± 0.086
3.649GluThr: 3.649 ± 0.104
5.316GluVal: 5.316 ± 0.107
0.468GluTrp: 0.468 ± 0.035
3.224GluTyr: 3.224 ± 0.081
0.0GluXaa: 0.0 ± 0.0
Phe
2.41PheAla: 2.41 ± 0.08
0.614PheCys: 0.614 ± 0.042
3.183PheAsp: 3.183 ± 0.09
3.154PheGlu: 3.154 ± 0.09
1.868PhePhe: 1.868 ± 0.071
2.791PheGly: 2.791 ± 0.086
0.629PheHis: 0.629 ± 0.03
4.31PheIle: 4.31 ± 0.112
3.629PheLys: 3.629 ± 0.112
3.808PheLeu: 3.808 ± 0.105
1.075PheMet: 1.075 ± 0.056
2.913PheAsn: 2.913 ± 0.091
1.4PhePro: 1.4 ± 0.06
0.823PheGln: 0.823 ± 0.042
1.339PheArg: 1.339 ± 0.057
2.824PheSer: 2.824 ± 0.094
1.994PheThr: 1.994 ± 0.062
2.584PheVal: 2.584 ± 0.083
0.235PheTrp: 0.235 ± 0.021
1.624PheTyr: 1.624 ± 0.059
0.0PheXaa: 0.0 ± 0.0
Gly
4.28GlyAla: 4.28 ± 0.139
0.932GlyCys: 0.932 ± 0.052
3.903GlyAsp: 3.903 ± 0.097
4.58GlyGlu: 4.58 ± 0.118
3.287GlyPhe: 3.287 ± 0.078
4.173GlyGly: 4.173 ± 0.119
1.095GlyHis: 1.095 ± 0.041
6.416GlyIle: 6.416 ± 0.146
4.611GlyLys: 4.611 ± 0.102
5.408GlyLeu: 5.408 ± 0.106
1.665GlyMet: 1.665 ± 0.063
3.196GlyAsn: 3.196 ± 0.108
1.652GlyPro: 1.652 ± 0.066
1.411GlyGln: 1.411 ± 0.053
2.286GlyArg: 2.286 ± 0.073
4.115GlySer: 4.115 ± 0.114
3.363GlyThr: 3.363 ± 0.079
4.77GlyVal: 4.77 ± 0.127
0.433GlyTrp: 0.433 ± 0.033
2.595GlyTyr: 2.595 ± 0.067
0.0GlyXaa: 0.0 ± 0.0
His
0.862HisAla: 0.862 ± 0.044
0.294HisCys: 0.294 ± 0.025
1.001HisAsp: 1.001 ± 0.05
1.324HisGlu: 1.324 ± 0.063
0.647HisPhe: 0.647 ± 0.04
1.239HisGly: 1.239 ± 0.055
0.411HisHis: 0.411 ± 0.047
1.341HisIle: 1.341 ± 0.053
1.01HisLys: 1.01 ± 0.051
1.341HisLeu: 1.341 ± 0.055
0.381HisMet: 0.381 ± 0.028
0.836HisAsn: 0.836 ± 0.043
0.851HisPro: 0.851 ± 0.039
0.37HisGln: 0.37 ± 0.03
0.581HisArg: 0.581 ± 0.033
1.001HisSer: 1.001 ± 0.049
0.721HisThr: 0.721 ± 0.039
1.012HisVal: 1.012 ± 0.043
0.1HisTrp: 0.1 ± 0.013
0.703HisTyr: 0.703 ± 0.041
0.0HisXaa: 0.0 ± 0.0
Ile
6.622IleAla: 6.622 ± 0.138
1.217IleCys: 1.217 ± 0.051
6.35IleAsp: 6.35 ± 0.119
7.193IleGlu: 7.193 ± 0.136
4.067IlePhe: 4.067 ± 0.097
6.091IleGly: 6.091 ± 0.151
1.543IleHis: 1.543 ± 0.046
8.867IleIle: 8.867 ± 0.178
7.498IleLys: 7.498 ± 0.168
8.566IleLeu: 8.566 ± 0.17
2.031IleMet: 2.031 ± 0.073
5.732IleAsn: 5.732 ± 0.141
3.703IlePro: 3.703 ± 0.104
1.857IleGln: 1.857 ± 0.077
2.956IleArg: 2.956 ± 0.073
6.394IleSer: 6.394 ± 0.114
4.765IleThr: 4.765 ± 0.116
6.211IleVal: 6.211 ± 0.125
0.522IleTrp: 0.522 ± 0.033
3.1IleTyr: 3.1 ± 0.094
0.0IleXaa: 0.0 ± 0.0
Lys
4.319LysAla: 4.319 ± 0.104
0.882LysCys: 0.882 ± 0.061
5.479LysAsp: 5.479 ± 0.127
7.876LysGlu: 7.876 ± 0.151
2.939LysPhe: 2.939 ± 0.091
4.454LysGly: 4.454 ± 0.099
1.18LysHis: 1.18 ± 0.053
7.735LysIle: 7.735 ± 0.153
7.071LysLys: 7.071 ± 0.167
6.664LysLeu: 6.664 ± 0.151
1.946LysMet: 1.946 ± 0.074
5.003LysAsn: 5.003 ± 0.122
2.393LysPro: 2.393 ± 0.077
1.748LysGln: 1.748 ± 0.06
2.834LysArg: 2.834 ± 0.093
4.541LysSer: 4.541 ± 0.11
3.988LysThr: 3.988 ± 0.103
4.852LysVal: 4.852 ± 0.124
0.488LysTrp: 0.488 ± 0.036
3.453LysTyr: 3.453 ± 0.089
0.0LysXaa: 0.0 ± 0.0
Leu
5.752LeuAla: 5.752 ± 0.132
1.004LeuCys: 1.004 ± 0.053
6.076LeuAsp: 6.076 ± 0.138
6.69LeuGlu: 6.69 ± 0.142
3.786LeuPhe: 3.786 ± 0.114
5.599LeuGly: 5.599 ± 0.124
1.289LeuHis: 1.289 ± 0.052
8.31LeuIle: 8.31 ± 0.162
8.183LeuLys: 8.183 ± 0.164
6.986LeuLeu: 6.986 ± 0.148
2.162LeuMet: 2.162 ± 0.066
6.096LeuAsn: 6.096 ± 0.134
2.824LeuPro: 2.824 ± 0.084
1.626LeuGln: 1.626 ± 0.058
2.978LeuArg: 2.978 ± 0.091
5.908LeuSer: 5.908 ± 0.133
4.7LeuThr: 4.7 ± 0.098
5.438LeuVal: 5.438 ± 0.122
0.501LeuTrp: 0.501 ± 0.03
2.554LeuTyr: 2.554 ± 0.08
0.0LeuXaa: 0.0 ± 0.0
Met
1.731MetAla: 1.731 ± 0.063
0.272MetCys: 0.272 ± 0.025
1.8MetAsp: 1.8 ± 0.061
1.89MetGlu: 1.89 ± 0.065
0.893MetPhe: 0.893 ± 0.047
1.733MetGly: 1.733 ± 0.069
0.425MetHis: 0.425 ± 0.031
2.031MetIle: 2.031 ± 0.075
1.909MetLys: 1.909 ± 0.065
1.857MetLeu: 1.857 ± 0.063
0.575MetMet: 0.575 ± 0.033
1.452MetAsn: 1.452 ± 0.058
0.853MetPro: 0.853 ± 0.045
0.488MetGln: 0.488 ± 0.032
0.801MetArg: 0.801 ± 0.04
1.611MetSer: 1.611 ± 0.063
1.051MetThr: 1.051 ± 0.048
1.587MetVal: 1.587 ± 0.062
0.155MetTrp: 0.155 ± 0.017
0.729MetTyr: 0.729 ± 0.047
0.0MetXaa: 0.0 ± 0.0
Asn
2.869AsnAla: 2.869 ± 0.087
0.812AsnCys: 0.812 ± 0.052
3.407AsnAsp: 3.407 ± 0.1
4.722AsnGlu: 4.722 ± 0.107
2.403AsnPhe: 2.403 ± 0.086
3.383AsnGly: 3.383 ± 0.124
0.977AsnHis: 0.977 ± 0.046
6.159AsnIle: 6.159 ± 0.139
4.94AsnLys: 4.94 ± 0.129
5.665AsnLeu: 5.665 ± 0.131
1.411AsnMet: 1.411 ± 0.055
4.003AsnAsn: 4.003 ± 0.168
2.719AsnPro: 2.719 ± 0.082
1.718AsnGln: 1.718 ± 0.069
1.763AsnArg: 1.763 ± 0.068
4.023AsnSer: 4.023 ± 0.119
2.676AsnThr: 2.676 ± 0.083
3.72AsnVal: 3.72 ± 0.102
0.416AsnTrp: 0.416 ± 0.031
2.408AsnTyr: 2.408 ± 0.084
0.0AsnXaa: 0.0 ± 0.0
Pro
1.755ProAla: 1.755 ± 0.071
0.39ProCys: 0.39 ± 0.031
2.101ProAsp: 2.101 ± 0.069
3.035ProGlu: 3.035 ± 0.094
1.681ProPhe: 1.681 ± 0.072
2.151ProGly: 2.151 ± 0.088
0.69ProHis: 0.69 ± 0.046
3.046ProIle: 3.046 ± 0.081
2.369ProLys: 2.369 ± 0.078
3.085ProLeu: 3.085 ± 0.078
0.731ProMet: 0.731 ± 0.042
1.855ProAsn: 1.855 ± 0.068
0.875ProPro: 0.875 ± 0.042
0.951ProGln: 0.951 ± 0.054
1.049ProArg: 1.049 ± 0.048
2.077ProSer: 2.077 ± 0.074
1.789ProThr: 1.789 ± 0.063
2.471ProVal: 2.471 ± 0.078
0.224ProTrp: 0.224 ± 0.023
1.413ProTyr: 1.413 ± 0.06
0.0ProXaa: 0.0 ± 0.0
Gln
1.348GlnAla: 1.348 ± 0.056
0.274GlnCys: 0.274 ± 0.024
1.247GlnAsp: 1.247 ± 0.052
1.646GlnGlu: 1.646 ± 0.062
0.849GlnPhe: 0.849 ± 0.04
1.252GlnGly: 1.252 ± 0.06
0.372GlnHis: 0.372 ± 0.028
2.014GlnIle: 2.014 ± 0.075
1.824GlnLys: 1.824 ± 0.066
2.099GlnLeu: 2.099 ± 0.066
0.662GlnMet: 0.662 ± 0.04
1.2GlnAsn: 1.2 ± 0.049
0.601GlnPro: 0.601 ± 0.034
0.647GlnGln: 0.647 ± 0.048
0.897GlnArg: 0.897 ± 0.046
1.328GlnSer: 1.328 ± 0.055
1.025GlnThr: 1.025 ± 0.052
1.256GlnVal: 1.256 ± 0.052
0.226GlnTrp: 0.226 ± 0.022
0.982GlnTyr: 0.982 ± 0.042
0.0GlnXaa: 0.0 ± 0.0
Arg
2.001ArgAla: 2.001 ± 0.079
0.462ArgCys: 0.462 ± 0.03
2.081ArgAsp: 2.081 ± 0.071
2.776ArgGlu: 2.776 ± 0.075
1.441ArgPhe: 1.441 ± 0.057
2.083ArgGly: 2.083 ± 0.077
0.503ArgHis: 0.503 ± 0.038
2.771ArgIle: 2.771 ± 0.079
2.765ArgLys: 2.765 ± 0.075
2.85ArgLeu: 2.85 ± 0.084
0.825ArgMet: 0.825 ± 0.045
1.881ArgAsn: 1.881 ± 0.067
1.08ArgPro: 1.08 ± 0.051
0.869ArgGln: 0.869 ± 0.043
1.334ArgArg: 1.334 ± 0.061
1.659ArgSer: 1.659 ± 0.065
1.55ArgThr: 1.55 ± 0.067
2.066ArgVal: 2.066 ± 0.065
0.196ArgTrp: 0.196 ± 0.022
1.202ArgTyr: 1.202 ± 0.053
0.0ArgXaa: 0.0 ± 0.0
Ser
3.3SerAla: 3.3 ± 0.086
0.712SerCys: 0.712 ± 0.041
4.023SerAsp: 4.023 ± 0.096
4.532SerGlu: 4.532 ± 0.107
3.056SerPhe: 3.056 ± 0.098
4.241SerGly: 4.241 ± 0.113
1.117SerHis: 1.117 ± 0.048
5.569SerIle: 5.569 ± 0.12
5.506SerLys: 5.506 ± 0.118
5.969SerLeu: 5.969 ± 0.129
1.328SerMet: 1.328 ± 0.055
3.527SerAsn: 3.527 ± 0.121
2.144SerPro: 2.144 ± 0.062
1.655SerGln: 1.655 ± 0.06
2.066SerArg: 2.066 ± 0.061
4.432SerSer: 4.432 ± 0.128
3.133SerThr: 3.133 ± 0.076
3.792SerVal: 3.792 ± 0.088
0.488SerTrp: 0.488 ± 0.033
2.612SerTyr: 2.612 ± 0.087
0.0SerXaa: 0.0 ± 0.0
Thr
3.122ThrAla: 3.122 ± 0.082
0.629ThrCys: 0.629 ± 0.041
2.863ThrAsp: 2.863 ± 0.088
2.969ThrGlu: 2.969 ± 0.09
2.086ThrPhe: 2.086 ± 0.077
3.646ThrGly: 3.646 ± 0.09
0.849ThrHis: 0.849 ± 0.039
4.458ThrIle: 4.458 ± 0.109
3.139ThrLys: 3.139 ± 0.082
4.535ThrLeu: 4.535 ± 0.103
1.032ThrMet: 1.032 ± 0.044
2.48ThrAsn: 2.48 ± 0.075
2.275ThrPro: 2.275 ± 0.069
1.095ThrGln: 1.095 ± 0.055
1.633ThrArg: 1.633 ± 0.075
3.289ThrSer: 3.289 ± 0.093
2.81ThrThr: 2.81 ± 0.086
3.72ThrVal: 3.72 ± 0.112
0.285ThrTrp: 0.285 ± 0.023
1.761ThrTyr: 1.761 ± 0.06
0.0ThrXaa: 0.0 ± 0.0
Val
4.443ValAla: 4.443 ± 0.11
0.986ValCys: 0.986 ± 0.048
4.802ValAsp: 4.802 ± 0.099
5.118ValGlu: 5.118 ± 0.109
2.665ValPhe: 2.665 ± 0.079
4.43ValGly: 4.43 ± 0.1
1.028ValHis: 1.028 ± 0.048
6.309ValIle: 6.309 ± 0.126
5.02ValLys: 5.02 ± 0.123
5.715ValLeu: 5.715 ± 0.115
1.472ValMet: 1.472 ± 0.063
3.834ValAsn: 3.834 ± 0.1
2.358ValPro: 2.358 ± 0.073
1.352ValGln: 1.352 ± 0.054
1.89ValArg: 1.89 ± 0.069
4.441ValSer: 4.441 ± 0.114
3.163ValThr: 3.163 ± 0.104
4.883ValVal: 4.883 ± 0.128
0.324ValTrp: 0.324 ± 0.03
2.184ValTyr: 2.184 ± 0.085
0.0ValXaa: 0.0 ± 0.0
Trp
0.401TrpAla: 0.401 ± 0.031
0.076TrpCys: 0.076 ± 0.013
0.512TrpAsp: 0.512 ± 0.037
0.418TrpGlu: 0.418 ± 0.031
0.248TrpPhe: 0.248 ± 0.021
0.442TrpGly: 0.442 ± 0.029
0.096TrpHis: 0.096 ± 0.015
0.629TrpIle: 0.629 ± 0.033
0.494TrpLys: 0.494 ± 0.039
0.54TrpLeu: 0.54 ± 0.037
0.185TrpMet: 0.185 ± 0.02
0.407TrpAsn: 0.407 ± 0.03
0.148TrpPro: 0.148 ± 0.014
0.161TrpGln: 0.161 ± 0.02
0.231TrpArg: 0.231 ± 0.022
0.34TrpSer: 0.34 ± 0.028
0.246TrpThr: 0.246 ± 0.029
0.416TrpVal: 0.416 ± 0.034
0.085TrpTrp: 0.085 ± 0.014
0.22TrpTyr: 0.22 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.905TyrAla: 1.905 ± 0.072
0.636TyrCys: 0.636 ± 0.043
2.784TyrAsp: 2.784 ± 0.09
2.747TyrGlu: 2.747 ± 0.093
1.759TyrPhe: 1.759 ± 0.072
2.71TyrGly: 2.71 ± 0.088
0.627TyrHis: 0.627 ± 0.036
2.935TyrIle: 2.935 ± 0.081
2.765TyrLys: 2.765 ± 0.082
3.588TyrLeu: 3.588 ± 0.094
0.823TyrMet: 0.823 ± 0.046
2.571TyrAsn: 2.571 ± 0.095
1.476TyrPro: 1.476 ± 0.06
0.901TyrGln: 0.901 ± 0.047
1.226TyrArg: 1.226 ± 0.061
2.512TyrSer: 2.512 ± 0.087
1.807TyrThr: 1.807 ± 0.066
2.234TyrVal: 2.234 ± 0.068
0.298TyrTrp: 0.298 ± 0.024
1.604TyrTyr: 1.604 ± 0.068
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1581 proteins (459352 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski