Methylobacterium sp.

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Methylobacterium; unclassified Methylobacterium

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6219 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A519YE89|A0A519YE89_9RHIZ Malonate transporter subunit MadM OS=Methylobacterium sp. OX=409 GN=madM PE=4 SV=1
MM1 pKa = 7.33ITDD4 pKa = 3.97LWYY7 pKa = 10.58KK8 pKa = 10.46NAVIYY13 pKa = 9.86CLSVATFMDD22 pKa = 4.17ADD24 pKa = 3.96GDD26 pKa = 4.54GIGDD30 pKa = 3.78FAGLEE35 pKa = 3.84RR36 pKa = 11.84RR37 pKa = 11.84LDD39 pKa = 3.75YY40 pKa = 11.22LQGLGVTAVWLMPFQPSPP58 pKa = 3.27

Molecular weight: 6.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A519YNS3|A0A519YNS3_9RHIZ DMT family transporter (Fragment) OS=Methylobacterium sp. OX=409 GN=EOO66_01555 PE=4 SV=1
MM1 pKa = 7.3TKK3 pKa = 9.26TRR5 pKa = 11.84FTASAIAAAAFGAVLMSAGAVSAATPAPAPSLVQPEE41 pKa = 4.39APTVQVRR48 pKa = 11.84MTRR51 pKa = 11.84RR52 pKa = 11.84SMMRR56 pKa = 11.84HH57 pKa = 3.86HH58 pKa = 6.78TMRR61 pKa = 11.84RR62 pKa = 11.84HH63 pKa = 4.08MMRR66 pKa = 11.84RR67 pKa = 11.84GGGRR71 pKa = 11.84TISTKK76 pKa = 10.5LGARR80 pKa = 11.84TRR82 pKa = 3.68

Molecular weight: 8.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6219

0

6219

1327713

20

921

213.5

22.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.858 ± 0.046

0.833 ± 0.011

5.66 ± 0.023

5.254 ± 0.033

3.439 ± 0.022

9.194 ± 0.035

2.026 ± 0.014

4.539 ± 0.021

2.623 ± 0.027

10.222 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.271 ± 0.014

2.17 ± 0.017

5.663 ± 0.027

2.814 ± 0.018

7.805 ± 0.037

4.826 ± 0.021

5.534 ± 0.022

7.775 ± 0.03

1.295 ± 0.014

2.2 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski