Paenibacillus nuruki

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4645 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E3L5P2|A0A1E3L5P2_9BACL Carrier domain-containing protein OS=Paenibacillus nuruki OX=1886670 GN=PTI45_01503 PE=4 SV=1
MM1 pKa = 7.14TVSLDD6 pKa = 3.31QLTIQDD12 pKa = 4.08IPWNRR17 pKa = 11.84ITTPYY22 pKa = 10.44GRR24 pKa = 11.84ATEE27 pKa = 4.32VPQMIAKK34 pKa = 10.0RR35 pKa = 11.84NYY37 pKa = 9.22SGLSNIVEE45 pKa = 4.36HH46 pKa = 6.98QSTLWQVTPWLLFFLLKK63 pKa = 10.26EE64 pKa = 4.58LSLQSPDD71 pKa = 3.23EE72 pKa = 4.22VSAAEE77 pKa = 3.75IDD79 pKa = 4.3LYY81 pKa = 11.48AGVIEE86 pKa = 4.64ALSEE90 pKa = 4.07HH91 pKa = 6.28EE92 pKa = 4.03QLEE95 pKa = 4.5DD96 pKa = 3.77TEE98 pKa = 4.43QTLDD102 pKa = 3.75AMPLLLEE109 pKa = 4.27EE110 pKa = 4.55TYY112 pKa = 10.96LWDD115 pKa = 5.54NDD117 pKa = 4.03DD118 pKa = 5.07QDD120 pKa = 6.45DD121 pKa = 3.94EE122 pKa = 5.76LEE124 pKa = 4.18WDD126 pKa = 3.54NEE128 pKa = 3.99EE129 pKa = 3.85PRR131 pKa = 11.84GYY133 pKa = 10.71DD134 pKa = 3.05SEE136 pKa = 5.29IFFNYY141 pKa = 9.46YY142 pKa = 9.34VYY144 pKa = 10.8SYY146 pKa = 11.42LLLKK150 pKa = 10.15QALPIFEE157 pKa = 4.77NISRR161 pKa = 11.84HH162 pKa = 4.5TTDD165 pKa = 4.11QDD167 pKa = 3.62MQNTLQDD174 pKa = 3.35CMEE177 pKa = 4.2YY178 pKa = 11.1LEE180 pKa = 4.53

Molecular weight: 21.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E3L6Q9|A0A1E3L6Q9_9BACL Purine-nucleoside phosphorylase OS=Paenibacillus nuruki OX=1886670 GN=punA PE=4 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84PTFRR6 pKa = 11.84PNVRR10 pKa = 11.84KK11 pKa = 9.69RR12 pKa = 11.84SKK14 pKa = 9.51VHH16 pKa = 5.89GFRR19 pKa = 11.84KK20 pKa = 10.02RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4645

0

4645

1523825

29

5777

328.1

36.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.572 ± 0.036

0.705 ± 0.011

5.285 ± 0.028

6.517 ± 0.047

3.903 ± 0.029

6.708 ± 0.039

2.389 ± 0.022

7.478 ± 0.035

5.224 ± 0.034

9.708 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.838 ± 0.017

4.201 ± 0.03

3.872 ± 0.023

4.848 ± 0.037

4.307 ± 0.031

6.621 ± 0.031

6.042 ± 0.035

6.808 ± 0.028

1.248 ± 0.016

3.725 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski