Amino acid dipepetide frequency for Hypsibius dujardini (Water bear) (Macrobiotus dujardini)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.531AlaAla: 8.531 ± 0.068
1.292AlaCys: 1.292 ± 0.017
4.164AlaAsp: 4.164 ± 0.025
4.579AlaGlu: 4.579 ± 0.032
3.112AlaPhe: 3.112 ± 0.025
5.258AlaGly: 5.258 ± 0.039
1.554AlaHis: 1.554 ± 0.018
3.697AlaIle: 3.697 ± 0.027
3.922AlaLys: 3.922 ± 0.035
6.776AlaLeu: 6.776 ± 0.035
1.772AlaMet: 1.772 ± 0.017
2.914AlaAsn: 2.914 ± 0.023
4.128AlaPro: 4.128 ± 0.045
2.749AlaGln: 2.749 ± 0.028
3.9AlaArg: 3.9 ± 0.029
6.273AlaSer: 6.273 ± 0.036
4.998AlaThr: 4.998 ± 0.044
6.253AlaVal: 6.253 ± 0.039
0.822AlaTrp: 0.822 ± 0.011
1.835AlaTyr: 1.835 ± 0.019
0.0AlaXaa: 0.0 ± 0.0
Cys
1.22CysAla: 1.22 ± 0.016
0.533CysCys: 0.533 ± 0.012
1.067CysAsp: 1.067 ± 0.02
0.866CysGlu: 0.866 ± 0.016
0.831CysPhe: 0.831 ± 0.013
1.493CysGly: 1.493 ± 0.022
0.461CysHis: 0.461 ± 0.01
0.883CysIle: 0.883 ± 0.016
0.773CysLys: 0.773 ± 0.013
1.891CysLeu: 1.891 ± 0.022
0.369CysMet: 0.369 ± 0.008
0.703CysAsn: 0.703 ± 0.012
1.06CysPro: 1.06 ± 0.019
0.761CysGln: 0.761 ± 0.013
1.187CysArg: 1.187 ± 0.015
1.503CysSer: 1.503 ± 0.019
0.971CysThr: 0.971 ± 0.015
1.216CysVal: 1.216 ± 0.018
0.281CysTrp: 0.281 ± 0.006
0.535CysTyr: 0.535 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
4.045AspAla: 4.045 ± 0.032
1.015AspCys: 1.015 ± 0.018
3.69AspAsp: 3.69 ± 0.058
3.772AspGlu: 3.772 ± 0.048
2.467AspPhe: 2.467 ± 0.021
4.074AspGly: 4.074 ± 0.035
1.309AspHis: 1.309 ± 0.017
2.725AspIle: 2.725 ± 0.021
2.272AspLys: 2.272 ± 0.022
5.083AspLeu: 5.083 ± 0.031
1.155AspMet: 1.155 ± 0.014
1.924AspAsn: 1.924 ± 0.017
2.776AspPro: 2.776 ± 0.021
1.976AspGln: 1.976 ± 0.019
3.139AspArg: 3.139 ± 0.027
4.11AspSer: 4.11 ± 0.027
2.47AspThr: 2.47 ± 0.02
3.748AspVal: 3.748 ± 0.028
0.719AspTrp: 0.719 ± 0.012
1.676AspTyr: 1.676 ± 0.018
0.0AspXaa: 0.0 ± 0.0
Glu
4.276GluAla: 4.276 ± 0.037
0.873GluCys: 0.873 ± 0.018
3.66GluAsp: 3.66 ± 0.044
4.913GluGlu: 4.913 ± 0.062
2.101GluPhe: 2.101 ± 0.019
2.853GluGly: 2.853 ± 0.022
1.235GluHis: 1.235 ± 0.015
3.008GluIle: 3.008 ± 0.026
3.656GluLys: 3.656 ± 0.035
5.034GluLeu: 5.034 ± 0.039
1.527GluMet: 1.527 ± 0.016
2.445GluAsn: 2.445 ± 0.025
2.55GluPro: 2.55 ± 0.024
2.339GluGln: 2.339 ± 0.023
3.496GluArg: 3.496 ± 0.031
3.928GluSer: 3.928 ± 0.031
3.241GluThr: 3.241 ± 0.029
3.456GluVal: 3.456 ± 0.028
0.697GluTrp: 0.697 ± 0.011
1.535GluTyr: 1.535 ± 0.015
0.0GluXaa: 0.0 ± 0.0
Phe
3.084PheAla: 3.084 ± 0.025
0.944PheCys: 0.944 ± 0.013
2.274PheAsp: 2.274 ± 0.019
2.035PheGlu: 2.035 ± 0.019
1.958PhePhe: 1.958 ± 0.021
3.036PheGly: 3.036 ± 0.032
1.119PheHis: 1.119 ± 0.014
2.131PheIle: 2.131 ± 0.021
1.594PheLys: 1.594 ± 0.016
4.159PheLeu: 4.159 ± 0.036
0.909PheMet: 0.909 ± 0.013
1.685PheAsn: 1.685 ± 0.015
2.212PhePro: 2.212 ± 0.022
1.64PheGln: 1.64 ± 0.014
2.508PheArg: 2.508 ± 0.02
3.301PheSer: 3.301 ± 0.034
2.366PheThr: 2.366 ± 0.02
2.914PheVal: 2.914 ± 0.025
0.562PheTrp: 0.562 ± 0.011
1.277PheTyr: 1.277 ± 0.014
0.0PheXaa: 0.0 ± 0.0
Gly
4.382GlyAla: 4.382 ± 0.032
1.192GlyCys: 1.192 ± 0.016
3.442GlyAsp: 3.442 ± 0.029
3.199GlyGlu: 3.199 ± 0.029
2.815GlyPhe: 2.815 ± 0.025
6.488GlyGly: 6.488 ± 0.102
1.567GlyHis: 1.567 ± 0.017
3.201GlyIle: 3.201 ± 0.025
3.453GlyLys: 3.453 ± 0.032
5.452GlyLeu: 5.452 ± 0.038
1.633GlyMet: 1.633 ± 0.018
2.873GlyAsn: 2.873 ± 0.025
3.05GlyPro: 3.05 ± 0.035
2.59GlyGln: 2.59 ± 0.033
3.853GlyArg: 3.853 ± 0.027
5.847GlySer: 5.847 ± 0.047
3.859GlyThr: 3.859 ± 0.032
3.968GlyVal: 3.968 ± 0.029
0.828GlyTrp: 0.828 ± 0.013
2.02GlyTyr: 2.02 ± 0.029
0.0GlyXaa: 0.0 ± 0.0
His
1.572HisAla: 1.572 ± 0.019
0.549HisCys: 0.549 ± 0.009
1.156HisAsp: 1.156 ± 0.015
1.154HisGlu: 1.154 ± 0.015
1.111HisPhe: 1.111 ± 0.013
1.655HisGly: 1.655 ± 0.017
1.207HisHis: 1.207 ± 0.026
1.091HisIle: 1.091 ± 0.012
0.904HisLys: 0.904 ± 0.013
2.306HisLeu: 2.306 ± 0.02
0.483HisMet: 0.483 ± 0.008
0.944HisAsn: 0.944 ± 0.011
1.563HisPro: 1.563 ± 0.019
1.096HisGln: 1.096 ± 0.016
1.625HisArg: 1.625 ± 0.019
1.912HisSer: 1.912 ± 0.02
1.154HisThr: 1.154 ± 0.016
1.503HisVal: 1.503 ± 0.015
0.313HisTrp: 0.313 ± 0.009
0.751HisTyr: 0.751 ± 0.013
0.0HisXaa: 0.0 ± 0.0
Ile
3.753IleAla: 3.753 ± 0.03
1.092IleCys: 1.092 ± 0.014
2.399IleAsp: 2.399 ± 0.021
2.324IleGlu: 2.324 ± 0.021
2.337IlePhe: 2.337 ± 0.026
2.986IleGly: 2.986 ± 0.024
1.201IleHis: 1.201 ± 0.013
2.646IleIle: 2.646 ± 0.042
2.176IleLys: 2.176 ± 0.021
4.806IleLeu: 4.806 ± 0.035
1.033IleMet: 1.033 ± 0.014
1.96IleAsn: 1.96 ± 0.02
2.97IlePro: 2.97 ± 0.022
1.93IleGln: 1.93 ± 0.02
3.011IleArg: 3.011 ± 0.022
3.83IleSer: 3.83 ± 0.025
2.911IleThr: 2.911 ± 0.023
3.435IleVal: 3.435 ± 0.038
0.636IleTrp: 0.636 ± 0.011
1.408IleTyr: 1.408 ± 0.015
0.0IleXaa: 0.0 ± 0.0
Lys
3.67LysAla: 3.67 ± 0.034
0.858LysCys: 0.858 ± 0.016
2.734LysAsp: 2.734 ± 0.026
3.215LysGlu: 3.215 ± 0.03
1.85LysPhe: 1.85 ± 0.018
2.498LysGly: 2.498 ± 0.03
1.064LysHis: 1.064 ± 0.015
2.519LysIle: 2.519 ± 0.024
3.611LysLys: 3.611 ± 0.039
4.404LysLeu: 4.404 ± 0.029
1.247LysMet: 1.247 ± 0.012
2.081LysAsn: 2.081 ± 0.017
2.631LysPro: 2.631 ± 0.033
1.929LysGln: 1.929 ± 0.017
3.321LysArg: 3.321 ± 0.031
3.7LysSer: 3.7 ± 0.032
3.062LysThr: 3.062 ± 0.025
3.002LysVal: 3.002 ± 0.025
0.623LysTrp: 0.623 ± 0.01
1.364LysTyr: 1.364 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
7.034LeuAla: 7.034 ± 0.036
1.611LeuCys: 1.611 ± 0.02
4.832LeuAsp: 4.832 ± 0.029
5.207LeuGlu: 5.207 ± 0.04
3.771LeuPhe: 3.771 ± 0.032
4.956LeuGly: 4.956 ± 0.031
2.335LeuHis: 2.335 ± 0.025
4.53LeuIle: 4.53 ± 0.032
4.655LeuLys: 4.655 ± 0.033
9.201LeuLeu: 9.201 ± 0.062
2.099LeuMet: 2.099 ± 0.021
3.666LeuAsn: 3.666 ± 0.024
5.402LeuPro: 5.402 ± 0.036
4.129LeuGln: 4.129 ± 0.032
5.765LeuArg: 5.765 ± 0.037
6.923LeuSer: 6.923 ± 0.036
5.593LeuThr: 5.593 ± 0.038
5.595LeuVal: 5.595 ± 0.033
1.021LeuTrp: 1.021 ± 0.013
2.378LeuTyr: 2.378 ± 0.021
0.0LeuXaa: 0.0 ± 0.0
Met
2.05MetAla: 2.05 ± 0.019
0.344MetCys: 0.344 ± 0.007
1.435MetAsp: 1.435 ± 0.014
1.625MetGlu: 1.625 ± 0.019
0.84MetPhe: 0.84 ± 0.012
1.344MetGly: 1.344 ± 0.017
0.47MetHis: 0.47 ± 0.009
1.032MetIle: 1.032 ± 0.014
1.233MetLys: 1.233 ± 0.015
1.928MetLeu: 1.928 ± 0.017
0.673MetMet: 0.673 ± 0.013
0.954MetAsn: 0.954 ± 0.013
1.189MetPro: 1.189 ± 0.014
0.936MetGln: 0.936 ± 0.015
1.25MetArg: 1.25 ± 0.014
1.746MetSer: 1.746 ± 0.015
1.457MetThr: 1.457 ± 0.014
1.411MetVal: 1.411 ± 0.016
0.263MetTrp: 0.263 ± 0.006
0.552MetTyr: 0.552 ± 0.01
0.0MetXaa: 0.0 ± 0.0
Asn
2.995AsnAla: 2.995 ± 0.024
0.823AsnCys: 0.823 ± 0.014
2.008AsnAsp: 2.008 ± 0.019
2.034AsnGlu: 2.034 ± 0.02
1.792AsnPhe: 1.792 ± 0.018
3.645AsnGly: 3.645 ± 0.035
0.971AsnHis: 0.971 ± 0.014
2.086AsnIle: 2.086 ± 0.019
1.614AsnLys: 1.614 ± 0.016
3.647AsnLeu: 3.647 ± 0.025
0.877AsnMet: 0.877 ± 0.015
1.988AsnAsn: 1.988 ± 0.026
2.351AsnPro: 2.351 ± 0.02
1.629AsnGln: 1.629 ± 0.021
2.407AsnArg: 2.407 ± 0.02
3.323AsnSer: 3.323 ± 0.028
1.982AsnThr: 1.982 ± 0.019
2.721AsnVal: 2.721 ± 0.022
0.566AsnTrp: 0.566 ± 0.011
1.232AsnTyr: 1.232 ± 0.014
0.0AsnXaa: 0.0 ± 0.0
Pro
4.932ProAla: 4.932 ± 0.045
0.774ProCys: 0.774 ± 0.015
2.998ProAsp: 2.998 ± 0.02
3.352ProGlu: 3.352 ± 0.029
2.202ProPhe: 2.202 ± 0.022
3.504ProGly: 3.504 ± 0.041
1.226ProHis: 1.226 ± 0.016
2.413ProIle: 2.413 ± 0.021
2.588ProLys: 2.588 ± 0.026
4.535ProLeu: 4.535 ± 0.029
1.083ProMet: 1.083 ± 0.015
2.234ProAsn: 2.234 ± 0.019
4.616ProPro: 4.616 ± 0.05
2.204ProGln: 2.204 ± 0.029
2.916ProArg: 2.916 ± 0.025
5.329ProSer: 5.329 ± 0.045
3.731ProThr: 3.731 ± 0.035
4.151ProVal: 4.151 ± 0.034
0.586ProTrp: 0.586 ± 0.011
1.455ProTyr: 1.455 ± 0.017
0.001ProXaa: 0.001 ± 0.0
Gln
2.934GlnAla: 2.934 ± 0.025
0.743GlnCys: 0.743 ± 0.016
1.766GlnAsp: 1.766 ± 0.02
2.206GlnGlu: 2.206 ± 0.024
1.629GlnPhe: 1.629 ± 0.018
1.965GlnGly: 1.965 ± 0.025
1.175GlnHis: 1.175 ± 0.016
1.992GlnIle: 1.992 ± 0.019
2.038GlnLys: 2.038 ± 0.023
3.932GlnLeu: 3.932 ± 0.031
1.036GlnMet: 1.036 ± 0.013
1.713GlnAsn: 1.713 ± 0.019
2.501GlnPro: 2.501 ± 0.032
3.462GlnGln: 3.462 ± 0.072
2.712GlnArg: 2.712 ± 0.025
3.111GlnSer: 3.111 ± 0.026
2.492GlnThr: 2.492 ± 0.024
2.207GlnVal: 2.207 ± 0.019
0.493GlnTrp: 0.493 ± 0.009
1.162GlnTyr: 1.162 ± 0.014
0.0GlnXaa: 0.0 ± 0.0
Arg
3.74ArgAla: 3.74 ± 0.025
1.132ArgCys: 1.132 ± 0.019
3.159ArgAsp: 3.159 ± 0.024
3.29ArgGlu: 3.29 ± 0.028
2.382ArgPhe: 2.382 ± 0.021
3.265ArgGly: 3.265 ± 0.027
1.592ArgHis: 1.592 ± 0.017
2.934ArgIle: 2.934 ± 0.019
3.617ArgLys: 3.617 ± 0.027
5.461ArgLeu: 5.461 ± 0.036
1.439ArgMet: 1.439 ± 0.014
2.762ArgAsn: 2.762 ± 0.021
3.389ArgPro: 3.389 ± 0.029
2.643ArgGln: 2.643 ± 0.028
4.743ArgArg: 4.743 ± 0.037
4.898ArgSer: 4.898 ± 0.038
3.548ArgThr: 3.548 ± 0.027
3.296ArgVal: 3.296 ± 0.024
0.741ArgTrp: 0.741 ± 0.011
1.608ArgTyr: 1.608 ± 0.017
0.0ArgXaa: 0.0 ± 0.0
Ser
6.626SerAla: 6.626 ± 0.041
1.442SerCys: 1.442 ± 0.019
4.338SerAsp: 4.338 ± 0.03
3.888SerGlu: 3.888 ± 0.03
3.233SerPhe: 3.233 ± 0.029
6.021SerGly: 6.021 ± 0.048
1.942SerHis: 1.942 ± 0.02
3.435SerIle: 3.435 ± 0.026
3.366SerLys: 3.366 ± 0.028
7.039SerLeu: 7.039 ± 0.037
1.661SerMet: 1.661 ± 0.018
3.093SerAsn: 3.093 ± 0.028
5.089SerPro: 5.089 ± 0.037
3.112SerGln: 3.112 ± 0.025
4.689SerArg: 4.689 ± 0.039
9.659SerSer: 9.659 ± 0.108
5.398SerThr: 5.398 ± 0.047
5.555SerVal: 5.555 ± 0.032
0.866SerTrp: 0.866 ± 0.012
2.091SerTyr: 2.091 ± 0.022
0.0SerXaa: 0.0 ± 0.0
Thr
5.515ThrAla: 5.515 ± 0.038
1.132ThrCys: 1.132 ± 0.018
3.265ThrAsp: 3.265 ± 0.026
3.207ThrGlu: 3.207 ± 0.031
2.562ThrPhe: 2.562 ± 0.024
4.084ThrGly: 4.084 ± 0.033
1.18ThrHis: 1.18 ± 0.014
2.972ThrIle: 2.972 ± 0.026
2.658ThrLys: 2.658 ± 0.024
5.159ThrLeu: 5.159 ± 0.034
1.216ThrMet: 1.216 ± 0.014
2.314ThrAsn: 2.314 ± 0.023
3.543ThrPro: 3.543 ± 0.026
1.941ThrGln: 1.941 ± 0.019
2.883ThrArg: 2.883 ± 0.021
5.183ThrSer: 5.183 ± 0.043
5.233ThrThr: 5.233 ± 0.088
4.853ThrVal: 4.853 ± 0.04
0.709ThrTrp: 0.709 ± 0.011
1.492ThrTyr: 1.492 ± 0.017
0.001ThrXaa: 0.001 ± 0.001
Val
5.568ValAla: 5.568 ± 0.033
1.406ValCys: 1.406 ± 0.02
3.59ValAsp: 3.59 ± 0.026
3.895ValGlu: 3.895 ± 0.029
2.868ValPhe: 2.868 ± 0.029
3.87ValGly: 3.87 ± 0.029
1.462ValHis: 1.462 ± 0.014
3.496ValIle: 3.496 ± 0.029
3.333ValLys: 3.333 ± 0.028
6.136ValLeu: 6.136 ± 0.04
1.57ValMet: 1.57 ± 0.015
2.574ValAsn: 2.574 ± 0.019
3.78ValPro: 3.78 ± 0.029
2.553ValGln: 2.553 ± 0.022
3.649ValArg: 3.649 ± 0.025
4.914ValSer: 4.914 ± 0.035
4.271ValThr: 4.271 ± 0.038
5.214ValVal: 5.214 ± 0.043
0.814ValTrp: 0.814 ± 0.012
1.895ValTyr: 1.895 ± 0.017
0.0ValXaa: 0.0 ± 0.0
Trp
0.707TrpAla: 0.707 ± 0.011
0.219TrpCys: 0.219 ± 0.006
0.649TrpAsp: 0.649 ± 0.011
0.614TrpGlu: 0.614 ± 0.01
0.495TrpPhe: 0.495 ± 0.01
0.561TrpGly: 0.561 ± 0.013
0.273TrpHis: 0.273 ± 0.007
0.749TrpIle: 0.749 ± 0.012
0.797TrpLys: 0.797 ± 0.012
1.173TrpLeu: 1.173 ± 0.016
0.375TrpMet: 0.375 ± 0.007
0.661TrpAsn: 0.661 ± 0.012
0.539TrpPro: 0.539 ± 0.01
0.48TrpGln: 0.48 ± 0.009
0.783TrpArg: 0.783 ± 0.012
0.965TrpSer: 0.965 ± 0.012
0.929TrpThr: 0.929 ± 0.014
0.607TrpVal: 0.607 ± 0.011
0.218TrpTrp: 0.218 ± 0.007
0.361TrpTyr: 0.361 ± 0.008
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.892TyrAla: 1.892 ± 0.017
0.63TyrCys: 0.63 ± 0.01
1.521TyrAsp: 1.521 ± 0.016
1.448TyrGlu: 1.448 ± 0.018
1.355TyrPhe: 1.355 ± 0.014
2.154TyrGly: 2.154 ± 0.029
0.708TyrHis: 0.708 ± 0.011
1.305TyrIle: 1.305 ± 0.013
1.122TyrLys: 1.122 ± 0.015
2.581TyrLeu: 2.581 ± 0.022
0.578TyrMet: 0.578 ± 0.009
1.201TyrAsn: 1.201 ± 0.016
1.491TyrPro: 1.491 ± 0.02
1.168TyrGln: 1.168 ± 0.014
1.802TyrArg: 1.802 ± 0.02
2.087TyrSer: 2.087 ± 0.021
1.435TyrThr: 1.435 ± 0.019
1.752TyrVal: 1.752 ± 0.017
0.398TyrTrp: 0.398 ± 0.008
1.018TyrTyr: 1.018 ± 0.015
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.001XaaAla: 0.001 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.001XaaPro: 0.001 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.001XaaArg: 0.001 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.001XaaThr: 0.001 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.024XaaXaa: 0.024 ± 0.004
Statistics based on 14867 proteins (6817457 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski