Actinomadura darangshiensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Thermomonosporaceae; Actinomadura

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9026 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R5AXU9|A0A4R5AXU9_9ACTN MFS transporter (Fragment) OS=Actinomadura darangshiensis OX=705336 GN=E1293_25140 PE=4 SV=1
MM1 pKa = 7.44TLLHH5 pKa = 6.88LPRR8 pKa = 11.84LTRR11 pKa = 11.84TAAAVAVLAAASSVLAPAASALPEE35 pKa = 4.3DD36 pKa = 4.38TWTVDD41 pKa = 2.98PGGALTAASPLSIQNVTRR59 pKa = 11.84GWSIDD64 pKa = 3.43CGQATFTGTAEE75 pKa = 4.39FGAGNQVDD83 pKa = 4.26PLVEE87 pKa = 4.12FDD89 pKa = 5.61DD90 pKa = 5.24EE91 pKa = 4.92SFEE94 pKa = 4.22NCAGPDD100 pKa = 3.59GVGYY104 pKa = 8.01TVTMTSGVQLEE115 pKa = 5.0MYY117 pKa = 10.18ASSYY121 pKa = 10.95DD122 pKa = 3.35AGAGKK127 pKa = 7.51TTGTLFGFQLNLVGTNRR144 pKa = 11.84CQADD148 pKa = 3.61VADD151 pKa = 4.12PAGNLGAADD160 pKa = 3.32ATFTNEE166 pKa = 3.62GSALGLGGGNMGVTFVNFACPSDD189 pKa = 4.71FIANGDD195 pKa = 3.67QVVIAARR202 pKa = 11.84LAVSPAQTITGSS214 pKa = 3.19

Molecular weight: 21.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V2YUI6|A0A4V2YUI6_9ACTN Phosphate transporter OS=Actinomadura darangshiensis OX=705336 GN=E1293_25275 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 5.34KK15 pKa = 10.47KK16 pKa = 8.84HH17 pKa = 5.5GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSGRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.5GRR40 pKa = 11.84ARR42 pKa = 11.84VAVV45 pKa = 3.65

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9026

0

9026

2877669

24

3984

318.8

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.552 ± 0.035

0.784 ± 0.007

6.124 ± 0.022

5.535 ± 0.025

2.841 ± 0.014

9.714 ± 0.026

2.226 ± 0.013

3.465 ± 0.016

2.139 ± 0.019

10.32 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.914 ± 0.008

1.758 ± 0.013

6.088 ± 0.024

2.572 ± 0.015

8.42 ± 0.028

4.811 ± 0.019

5.704 ± 0.021

8.45 ± 0.025

1.522 ± 0.011

2.06 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski