Methanothermobacter phage psiM100

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9HH53|Q9HH53_9CAUD Putative portal protein OS=Methanothermobacter phage psiM100 OX=173824 GN=ORF10 PE=3 SV=1
MM1 pKa = 7.54ARR3 pKa = 11.84PGGIDD8 pKa = 3.62YY9 pKa = 11.09NPEE12 pKa = 4.09KK13 pKa = 10.22LNSMIDD19 pKa = 3.56DD20 pKa = 4.06FMDD23 pKa = 3.72STEE26 pKa = 4.66HH27 pKa = 5.43IMNRR31 pKa = 11.84VRR33 pKa = 11.84VSSVEE38 pKa = 3.86AFIDD42 pKa = 3.69DD43 pKa = 4.17LQALTRR49 pKa = 11.84EE50 pKa = 4.1EE51 pKa = 4.67FIRR54 pKa = 11.84KK55 pKa = 9.13YY56 pKa = 10.19GVTRR60 pKa = 11.84RR61 pKa = 11.84EE62 pKa = 3.77YY63 pKa = 11.15DD64 pKa = 3.35EE65 pKa = 6.19LIAALEE71 pKa = 4.06DD72 pKa = 3.79MKK74 pKa = 11.49DD75 pKa = 3.32EE76 pKa = 5.28DD77 pKa = 3.78MDD79 pKa = 3.97EE80 pKa = 4.67APSEE84 pKa = 4.7DD85 pKa = 3.79EE86 pKa = 3.6TDD88 pKa = 3.48MRR90 pKa = 11.84GLIYY94 pKa = 10.43FLGLAVIYY102 pKa = 7.24LTRR105 pKa = 11.84DD106 pKa = 3.09AEE108 pKa = 4.05RR109 pKa = 11.84DD110 pKa = 3.66AEE112 pKa = 3.97IEE114 pKa = 3.92AYY116 pKa = 9.8RR117 pKa = 11.84RR118 pKa = 11.84HH119 pKa = 6.19AEE121 pKa = 3.91DD122 pKa = 3.24YY123 pKa = 11.43GRR125 pKa = 11.84DD126 pKa = 3.54LYY128 pKa = 11.59LNWVTMGDD136 pKa = 3.53DD137 pKa = 4.24HH138 pKa = 9.26VCDD141 pKa = 3.51TCQEE145 pKa = 4.18YY146 pKa = 11.0EE147 pKa = 4.09DD148 pKa = 4.25ASPYY152 pKa = 9.76RR153 pKa = 11.84VEE155 pKa = 4.56GFPHH159 pKa = 6.66IPHH162 pKa = 7.16PFCRR166 pKa = 11.84CHH168 pKa = 7.09PEE170 pKa = 4.08PCDD173 pKa = 3.79EE174 pKa = 4.65EE175 pKa = 5.11GNPIDD180 pKa = 5.7DD181 pKa = 4.14LSEE184 pKa = 4.04YY185 pKa = 10.65SGPDD189 pKa = 3.13LEE191 pKa = 4.83YY192 pKa = 10.36FDD194 pKa = 6.17EE195 pKa = 5.18IMEE198 pKa = 4.57DD199 pKa = 3.88FQQ201 pKa = 6.41

Molecular weight: 23.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9HH65|Q9HH65_9CAUD SWIM-type domain-containing protein OS=Methanothermobacter phage psiM100 OX=173824 GN=ORFA PE=4 SV=1
MM1 pKa = 7.61GGRR4 pKa = 11.84RR5 pKa = 11.84MQRR8 pKa = 11.84RR9 pKa = 11.84ICGRR13 pKa = 11.84RR14 pKa = 11.84DD15 pKa = 3.05LQKK18 pKa = 10.74AIKK21 pKa = 9.57EE22 pKa = 3.92LLRR25 pKa = 11.84EE26 pKa = 3.98YY27 pKa = 11.34GEE29 pKa = 4.29LSVEE33 pKa = 3.99EE34 pKa = 4.02LHH36 pKa = 7.57RR37 pKa = 11.84LLLHH41 pKa = 6.24NYY43 pKa = 8.86EE44 pKa = 4.38FGRR47 pKa = 11.84NYY49 pKa = 10.91DD50 pKa = 3.41VTRR53 pKa = 11.84QAVTIYY59 pKa = 8.69TRR61 pKa = 11.84RR62 pKa = 11.84VAATTGITVNKK73 pKa = 8.3YY74 pKa = 7.99GKK76 pKa = 8.82PVRR79 pKa = 11.84VYY81 pKa = 9.96TLEE84 pKa = 3.85AA85 pKa = 4.47

Molecular weight: 10.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

36

0

36

8902

85

1157

247.3

28.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.381 ± 0.457

0.719 ± 0.156

6.235 ± 0.377

9.166 ± 0.43

3.64 ± 0.223

6.605 ± 0.319

1.91 ± 0.162

6.942 ± 0.328

6.897 ± 0.593

9.211 ± 0.423

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.651 ± 0.225

4.1 ± 0.216

4.325 ± 0.309

2.539 ± 0.172

5.931 ± 0.327

6.021 ± 0.406

5.358 ± 0.238

6.549 ± 0.338

1.348 ± 0.189

4.471 ± 0.317
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski