Mycobacterium phage Jabbawokkie

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Avanivirus; unclassified Avanivirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 106 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5Y3U6|S5Y3U6_9CAUD Uncharacterized protein OS=Mycobacterium phage Jabbawokkie OX=1340820 GN=103 PE=4 SV=1
MM1 pKa = 7.93SDD3 pKa = 3.41PQSTRR8 pKa = 11.84DD9 pKa = 3.4RR10 pKa = 11.84LIANIEE16 pKa = 3.97NTIWNALEE24 pKa = 4.13QQDD27 pKa = 3.91GPYY30 pKa = 10.15VDD32 pKa = 5.56RR33 pKa = 11.84EE34 pKa = 4.09MGMVDD39 pKa = 3.51ASGAGLDD46 pKa = 3.74MTAVAAAVADD56 pKa = 3.53IFVDD60 pKa = 4.98SEE62 pKa = 5.14DD63 pKa = 3.74DD64 pKa = 4.06CSWCHH69 pKa = 6.11SPCAVGGWSEE79 pKa = 3.99PVDD82 pKa = 3.63VGSVVQSEE90 pKa = 4.24PNQEE94 pKa = 4.43GITDD98 pKa = 3.52VV99 pKa = 3.5

Molecular weight: 10.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5Y3Q7|S5Y3Q7_9CAUD Lysin A OS=Mycobacterium phage Jabbawokkie OX=1340820 GN=38 PE=4 SV=1
MM1 pKa = 7.7KK2 pKa = 10.18IHH4 pKa = 5.44VQSRR8 pKa = 11.84GPAGWNATVLFTAGTVYY25 pKa = 10.11TVADD29 pKa = 3.79DD30 pKa = 3.38QGRR33 pKa = 11.84KK34 pKa = 9.22HH35 pKa = 6.73LIDD38 pKa = 3.44TSRR41 pKa = 11.84VAIRR45 pKa = 11.84RR46 pKa = 11.84LSS48 pKa = 3.2

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

106

0

106

17725

35

1183

167.2

18.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.804 ± 0.404

1.371 ± 0.184

6.347 ± 0.246

6.11 ± 0.331

2.872 ± 0.134

8.7 ± 0.721

2.127 ± 0.15

4.48 ± 0.16

3.622 ± 0.205

7.735 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.161 ± 0.145

3.069 ± 0.163

5.811 ± 0.196

3.583 ± 0.227

6.894 ± 0.433

6.087 ± 0.308

6.347 ± 0.304

7.25 ± 0.349

2.358 ± 0.159

2.274 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski