SARS coronavirus PUMC02

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Sarbecovirus; Severe acute respiratory syndrome-related coronavirus

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6UZF5|Q6UZF5_SARS 2'-O-methyltransferase OS=SARS coronavirus PUMC02 OX=253434 PE=3 SV=1
MM1 pKa = 7.88FIFLLFLTLTSGSDD15 pKa = 3.56LDD17 pKa = 3.81RR18 pKa = 11.84CTTFDD23 pKa = 5.11DD24 pKa = 4.16VQAPNYY30 pKa = 6.73TQHH33 pKa = 5.96TSSMRR38 pKa = 11.84GVYY41 pKa = 10.09YY42 pKa = 9.5PDD44 pKa = 3.78EE45 pKa = 4.48IFRR48 pKa = 11.84SDD50 pKa = 3.65TLYY53 pKa = 9.95LTQDD57 pKa = 3.75LFLPFYY63 pKa = 11.15SNVTGFHH70 pKa = 6.93TINHH74 pKa = 5.98TFGNPVIPFKK84 pKa = 11.08DD85 pKa = 3.93GIYY88 pKa = 10.18FAATEE93 pKa = 4.03KK94 pKa = 11.08SNVVRR99 pKa = 11.84GWVFGSTMNNKK110 pKa = 8.76SQSVIIINNSTNVVIRR126 pKa = 11.84ACNFEE131 pKa = 4.97LCDD134 pKa = 3.57NPFFAVSKK142 pKa = 10.56PMGTQTHH149 pKa = 5.42TMIFDD154 pKa = 3.4NAFNCTFEE162 pKa = 4.55YY163 pKa = 10.53ISDD166 pKa = 3.88AFSLDD171 pKa = 3.34VSEE174 pKa = 4.82KK175 pKa = 10.62SGNFKK180 pKa = 10.48HH181 pKa = 6.67LRR183 pKa = 11.84EE184 pKa = 4.1FVFKK188 pKa = 11.13NKK190 pKa = 10.4DD191 pKa = 3.05GFLYY195 pKa = 10.4VYY197 pKa = 10.28KK198 pKa = 10.23GYY200 pKa = 10.7QPIDD204 pKa = 3.59VVRR207 pKa = 11.84DD208 pKa = 3.53LPSGFNTLKK217 pKa = 10.74PIFKK221 pKa = 10.35LPLGINITNFRR232 pKa = 11.84AILTAFSPAQDD243 pKa = 2.57IWGTSAAAYY252 pKa = 9.13FVGYY256 pKa = 9.98LKK258 pKa = 9.58PTTFMLKK265 pKa = 9.68YY266 pKa = 10.06DD267 pKa = 4.12EE268 pKa = 5.37NGTITDD274 pKa = 5.15AVDD277 pKa = 3.92CSQNPLAEE285 pKa = 4.52LKK287 pKa = 10.85CSVKK291 pKa = 10.59SFEE294 pKa = 4.13IDD296 pKa = 2.72KK297 pKa = 11.25GIYY300 pKa = 6.46QTSNFRR306 pKa = 11.84VVPSGDD312 pKa = 3.08VVRR315 pKa = 11.84FPNITNLCPFGEE327 pKa = 4.44VFNATKK333 pKa = 10.41FPSVYY338 pKa = 9.44AWEE341 pKa = 4.09RR342 pKa = 11.84KK343 pKa = 9.67KK344 pKa = 10.83ISNCVADD351 pKa = 4.37YY352 pKa = 11.15SVLYY356 pKa = 10.67NSTFFSTFKK365 pKa = 10.6CYY367 pKa = 10.02GVSATKK373 pKa = 10.88LNDD376 pKa = 3.32LCFSNVYY383 pKa = 10.62ADD385 pKa = 3.79SFVVKK390 pKa = 10.43GDD392 pKa = 3.78DD393 pKa = 3.46VRR395 pKa = 11.84QIAPGQTGVIADD407 pKa = 4.17YY408 pKa = 10.79NYY410 pKa = 11.15KK411 pKa = 10.75LPDD414 pKa = 3.8DD415 pKa = 4.41FMGCVLAWNTRR426 pKa = 11.84NIDD429 pKa = 3.36ATSTGNYY436 pKa = 7.96NYY438 pKa = 10.14KK439 pKa = 10.12YY440 pKa = 10.29RR441 pKa = 11.84YY442 pKa = 8.78LRR444 pKa = 11.84HH445 pKa = 5.69GKK447 pKa = 9.14LRR449 pKa = 11.84PFEE452 pKa = 4.59RR453 pKa = 11.84DD454 pKa = 2.59ISNVPFSPDD463 pKa = 3.26GKK465 pKa = 9.6PCTPPALNCYY475 pKa = 8.9WPLNDD480 pKa = 3.46YY481 pKa = 11.13GFYY484 pKa = 7.22TTTGIGYY491 pKa = 8.17QPYY494 pKa = 9.17RR495 pKa = 11.84VVVLSFEE502 pKa = 4.61LLNAPATVCGPKK514 pKa = 10.44LSTDD518 pKa = 4.57LIKK521 pKa = 10.91NQCVNFNFNGLTGTGVLTPSSKK543 pKa = 10.22RR544 pKa = 11.84FQPFQQFGRR553 pKa = 11.84DD554 pKa = 3.25VSDD557 pKa = 3.54FTDD560 pKa = 3.29SVRR563 pKa = 11.84DD564 pKa = 3.75PKK566 pKa = 10.71TSEE569 pKa = 3.88ILDD572 pKa = 3.48ISPCSFGGVSVITPGTNASSEE593 pKa = 4.47VAVLYY598 pKa = 10.73QDD600 pKa = 4.28VNCTDD605 pKa = 3.12VSTAIHH611 pKa = 7.03ADD613 pKa = 3.02QLTPAWRR620 pKa = 11.84IYY622 pKa = 9.61STGNNVFQTQAGCLIGAEE640 pKa = 4.41HH641 pKa = 7.11VDD643 pKa = 3.32TSYY646 pKa = 11.61EE647 pKa = 3.86CDD649 pKa = 3.16IPIGAGICASYY660 pKa = 8.45HH661 pKa = 4.57TVSLLRR667 pKa = 11.84STSQKK672 pKa = 10.89SIVAYY677 pKa = 9.62TMSLGADD684 pKa = 3.28SSIAYY689 pKa = 10.1SNNTIAIPTNFSISITTEE707 pKa = 3.64VMPVSMAKK715 pKa = 9.66TSVDD719 pKa = 3.3CNMYY723 pKa = 9.84ICGDD727 pKa = 3.6STEE730 pKa = 4.39CANLLLQYY738 pKa = 11.2GSFCTQLNRR747 pKa = 11.84ALSGIAAEE755 pKa = 4.17QDD757 pKa = 3.08RR758 pKa = 11.84NTRR761 pKa = 11.84EE762 pKa = 3.35VFAQVKK768 pKa = 9.02QMYY771 pKa = 7.48KK772 pKa = 9.69TPTLKK777 pKa = 10.84YY778 pKa = 9.33FGGFNFSQILPDD790 pKa = 3.84PLKK793 pKa = 8.1PTKK796 pKa = 10.2RR797 pKa = 11.84SFIEE801 pKa = 4.3DD802 pKa = 3.61LLFNKK807 pKa = 7.99VTLADD812 pKa = 3.57AGFMKK817 pKa = 10.37QYY819 pKa = 10.74GEE821 pKa = 4.31CLGDD825 pKa = 3.25INARR829 pKa = 11.84DD830 pKa = 4.56LICAQKK836 pKa = 10.77FNGLTVLPPLLTDD849 pKa = 4.9DD850 pKa = 4.88MIAAYY855 pKa = 8.09TAALVSGTATAGWTFGAGAALQIPFAMQMAYY886 pKa = 10.08RR887 pKa = 11.84FNGIGVTQNVLYY899 pKa = 10.46EE900 pKa = 4.09NQKK903 pKa = 10.62QIANQFNKK911 pKa = 10.46AISQIQEE918 pKa = 4.19SLTTTSTALGKK929 pKa = 10.37LQDD932 pKa = 3.95VVNQNAQALNTLVKK946 pKa = 10.38QLSSNFGAISSVLNDD961 pKa = 3.17ILSRR965 pKa = 11.84LDD967 pKa = 3.31KK968 pKa = 11.45VEE970 pKa = 5.19AEE972 pKa = 4.28VQIDD976 pKa = 3.56RR977 pKa = 11.84LITGRR982 pKa = 11.84LQSLQTYY989 pKa = 7.4VTQQLIRR996 pKa = 11.84AAEE999 pKa = 3.85IRR1001 pKa = 11.84ASANLAATKK1010 pKa = 9.96MSEE1013 pKa = 4.28CVLGQSKK1020 pKa = 10.12RR1021 pKa = 11.84VDD1023 pKa = 4.0FCGKK1027 pKa = 9.92GYY1029 pKa = 10.57HH1030 pKa = 6.84LMSFPQAAPHH1040 pKa = 5.76GVVFLHH1046 pKa = 5.25VTYY1049 pKa = 10.82VPSQEE1054 pKa = 4.9RR1055 pKa = 11.84NFTTAPAICHH1065 pKa = 5.73EE1066 pKa = 4.45GKK1068 pKa = 10.56AYY1070 pKa = 9.93FPRR1073 pKa = 11.84EE1074 pKa = 3.72GVFVFNGTSWFITQRR1089 pKa = 11.84NFFSPQIITTDD1100 pKa = 3.16NTFVSGNCDD1109 pKa = 3.2VVIGIINNTVYY1120 pKa = 10.97DD1121 pKa = 4.22PLQPEE1126 pKa = 4.19LDD1128 pKa = 3.85SFKK1131 pKa = 11.4EE1132 pKa = 3.91EE1133 pKa = 3.56LDD1135 pKa = 3.63KK1136 pKa = 11.53YY1137 pKa = 10.15FKK1139 pKa = 10.99NHH1141 pKa = 6.01TSPDD1145 pKa = 3.14VDD1147 pKa = 4.4LGDD1150 pKa = 3.31ISGINASVVNIQKK1163 pKa = 10.63EE1164 pKa = 3.92IDD1166 pKa = 3.67RR1167 pKa = 11.84LNEE1170 pKa = 3.5VAKK1173 pKa = 10.61NLNEE1177 pKa = 4.14SLIDD1181 pKa = 3.75LQEE1184 pKa = 4.17LGKK1187 pKa = 10.54YY1188 pKa = 6.98EE1189 pKa = 5.3QYY1191 pKa = 10.78IKK1193 pKa = 10.03WPWYY1197 pKa = 8.39VWLGFIAGLIAIVMVTILLCCMTSCCSCLKK1227 pKa = 10.16GACSCGSCCKK1237 pKa = 10.16FDD1239 pKa = 4.43EE1240 pKa = 5.39DD1241 pKa = 5.38DD1242 pKa = 4.38SEE1244 pKa = 4.36PVLKK1248 pKa = 10.36GVKK1251 pKa = 9.35LHH1253 pKa = 5.3YY1254 pKa = 9.44TT1255 pKa = 3.44

Molecular weight: 139.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6UZF3|Q6UZF3_SARS Membrane protein OS=SARS coronavirus PUMC02 OX=253434 GN=M PE=3 SV=1
MM1 pKa = 7.81SDD3 pKa = 4.42NGPQSNQRR11 pKa = 11.84SAPRR15 pKa = 11.84ITFGGPTDD23 pKa = 3.81STDD26 pKa = 3.04NNQNGGRR33 pKa = 11.84NGARR37 pKa = 11.84PKK39 pKa = 10.13QRR41 pKa = 11.84RR42 pKa = 11.84PQGLPNNTASWFTALTQHH60 pKa = 6.06GKK62 pKa = 10.36EE63 pKa = 3.96EE64 pKa = 4.07LRR66 pKa = 11.84FPRR69 pKa = 11.84GQGVPINTNSGPDD82 pKa = 3.62DD83 pKa = 3.51QIGYY87 pKa = 8.13YY88 pKa = 9.97RR89 pKa = 11.84RR90 pKa = 11.84ATRR93 pKa = 11.84RR94 pKa = 11.84VRR96 pKa = 11.84GGDD99 pKa = 3.09GKK101 pKa = 10.39MKK103 pKa = 9.92EE104 pKa = 4.5LSPRR108 pKa = 11.84WYY110 pKa = 10.01FYY112 pKa = 11.5YY113 pKa = 10.73LGTGPEE119 pKa = 3.63ASLPYY124 pKa = 9.9GANKK128 pKa = 9.97EE129 pKa = 4.24GIVWVATEE137 pKa = 4.37GALNTPKK144 pKa = 10.91DD145 pKa = 4.0HH146 pKa = 7.46IGTRR150 pKa = 11.84NPNNNAATVLQLPQGTTLPKK170 pKa = 10.34GFYY173 pKa = 10.67AEE175 pKa = 4.5GSRR178 pKa = 11.84GGSQASSRR186 pKa = 11.84SSSRR190 pKa = 11.84SRR192 pKa = 11.84GNSRR196 pKa = 11.84NSTPGSSRR204 pKa = 11.84GNSPARR210 pKa = 11.84MASGGGEE217 pKa = 3.84TALALLLLDD226 pKa = 4.89RR227 pKa = 11.84LNQLEE232 pKa = 4.58SKK234 pKa = 10.86VSGKK238 pKa = 10.04GQQQQGQTVTKK249 pKa = 9.98KK250 pKa = 10.44SAAEE254 pKa = 3.73ASKK257 pKa = 10.76KK258 pKa = 9.61PRR260 pKa = 11.84QKK262 pKa = 9.66RR263 pKa = 11.84TATKK267 pKa = 10.12QYY269 pKa = 11.12NVTQAFGRR277 pKa = 11.84RR278 pKa = 11.84GPEE281 pKa = 3.4QTQGNFGDD289 pKa = 3.74QDD291 pKa = 5.15LIRR294 pKa = 11.84QGTDD298 pKa = 2.96YY299 pKa = 11.27KK300 pKa = 10.5HH301 pKa = 6.32WPQIAQFAPSASAFFGMSRR320 pKa = 11.84IGMEE324 pKa = 4.16VTPSGTWLTYY334 pKa = 10.42HH335 pKa = 7.06GAIKK339 pKa = 10.61LDD341 pKa = 4.18DD342 pKa = 4.89KK343 pKa = 11.64DD344 pKa = 3.91PQFKK348 pKa = 11.14DD349 pKa = 3.7NVILLNKK356 pKa = 10.01HH357 pKa = 4.67IDD359 pKa = 3.35AYY361 pKa = 9.77KK362 pKa = 9.88TFPPTEE368 pKa = 3.95PKK370 pKa = 9.88KK371 pKa = 10.6DD372 pKa = 3.48KK373 pKa = 10.73KK374 pKa = 10.99KK375 pKa = 9.88KK376 pKa = 8.29TDD378 pKa = 3.29EE379 pKa = 4.34AQPLPQRR386 pKa = 11.84QKK388 pKa = 10.35KK389 pKa = 8.88QPTVTLLPAADD400 pKa = 3.84MDD402 pKa = 4.26DD403 pKa = 5.3FSRR406 pKa = 11.84QLQNSMSGASADD418 pKa = 3.63STQAA422 pKa = 3.04

Molecular weight: 46.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

8971

221

7073

2242.8

250.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.223 ± 0.206

3.077 ± 0.488

5.54 ± 0.282

4.57 ± 0.647

4.871 ± 0.562

6.22 ± 0.646

2.04 ± 0.428

5.016 ± 0.519

5.685 ± 0.458

9.252 ± 0.692

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.352 ± 0.311

5.406 ± 0.458

4.091 ± 0.471

3.656 ± 0.703

3.835 ± 0.58

6.699 ± 0.45

7.134 ± 0.301

7.781 ± 0.849

1.115 ± 0.194

4.437 ± 0.298
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski