Cycloclasticus sp. symbiont of Poecilosclerida sp. M

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Piscirickettsiaceae; Cycloclasticus; unclassified Cycloclasticus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1993 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X1QEJ3|A0A1X1QEJ3_9GAMM Pimeloyl-[acyl-carrier protein] methyl ester esterase OS=Cycloclasticus sp. symbiont of Poecilosclerida sp. M OX=1840537 GN=bioH PE=3 SV=1
MM1 pKa = 6.58EE2 pKa = 5.86TIRR5 pKa = 11.84KK6 pKa = 6.96TPLSLGVAAGLGLLLASTGAMAISTGTDD34 pKa = 3.09LNLSAKK40 pKa = 9.9ARR42 pKa = 11.84AGGMAGAAYY51 pKa = 8.83TMPQEE56 pKa = 3.77ASAAVFGNPATLTQFKK72 pKa = 9.74GINMNFGASYY82 pKa = 10.79LGLKK86 pKa = 10.23SIDD89 pKa = 3.62IEE91 pKa = 4.43TSSTIAALGGGVSTSSKK108 pKa = 10.88SDD110 pKa = 3.04ADD112 pKa = 3.69NYY114 pKa = 10.15IVPDD118 pKa = 4.6FGLTLQVSPNLVLGTGLEE136 pKa = 4.06VDD138 pKa = 3.88AGLGADD144 pKa = 4.08YY145 pKa = 10.81RR146 pKa = 11.84DD147 pKa = 4.49DD148 pKa = 5.29PISLLGTGLVTIPLNVEE165 pKa = 4.53LISFNANLAAAYY177 pKa = 8.63QATEE181 pKa = 3.97KK182 pKa = 10.9LSIGGAVTIGFALAQLGTTGPTGGGAGLTALLGSDD217 pKa = 4.7APTDD221 pKa = 3.88LDD223 pKa = 4.41GPGPLPAIGANLGINDD239 pKa = 4.19FGGTTSSVHH248 pKa = 7.35DD249 pKa = 3.6IGFSGSLGATYY260 pKa = 9.76EE261 pKa = 4.16VQEE264 pKa = 4.21GVMLSAAYY272 pKa = 7.38KK273 pKa = 9.64TKK275 pKa = 10.67HH276 pKa = 5.95EE277 pKa = 4.48YY278 pKa = 10.98NFTNILYY285 pKa = 10.07QDD287 pKa = 3.51TSLNGPSGNGAGLGSGAFGPDD308 pKa = 3.4YY309 pKa = 10.92QDD311 pKa = 4.58LSVAQPAEE319 pKa = 4.37VIVGVAFDD327 pKa = 3.78NVIAQGLLVEE337 pKa = 5.39ADD339 pKa = 3.92VVWKK343 pKa = 10.12NWSDD347 pKa = 2.98AATYY351 pKa = 9.87EE352 pKa = 4.35NAYY355 pKa = 10.61DD356 pKa = 3.81DD357 pKa = 4.17QFLFLLGAQYY367 pKa = 7.91EE368 pKa = 4.44TGDD371 pKa = 2.86WSFRR375 pKa = 11.84AGYY378 pKa = 10.09SYY380 pKa = 11.68AEE382 pKa = 5.37DD383 pKa = 3.8ILRR386 pKa = 11.84SDD388 pKa = 4.64PDD390 pKa = 3.58TTLDD394 pKa = 3.66SLVGLGTLPVGDD406 pKa = 4.78GGPFGNDD413 pKa = 2.32IVKK416 pKa = 10.15IAQATLLPVIWNHH429 pKa = 6.65TITAGVGYY437 pKa = 10.09AITDD441 pKa = 3.56AVSIDD446 pKa = 3.6AYY448 pKa = 10.38AAYY451 pKa = 10.34AFGEE455 pKa = 4.57EE456 pKa = 4.33EE457 pKa = 4.37TVDD460 pKa = 3.64LPKK463 pKa = 10.57IQDD466 pKa = 3.25AAVAYY471 pKa = 7.07VVPVTEE477 pKa = 4.76LQLEE481 pKa = 4.21AQVDD485 pKa = 3.94YY486 pKa = 11.16EE487 pKa = 4.55FMVGAGINIALPP499 pKa = 3.76

Molecular weight: 51.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X1QHJ0|A0A1X1QHJ0_9GAMM CopG family transcriptional regulator OS=Cycloclasticus sp. symbiont of Poecilosclerida sp. M OX=1840537 GN=A6F71_06825 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.08RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.81IINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.01GRR39 pKa = 11.84AVLSAA44 pKa = 3.96

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1993

0

1993

544741

35

1771

273.3

30.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.493 ± 0.054

1.227 ± 0.022

5.57 ± 0.04

6.398 ± 0.06

4.004 ± 0.04

7.131 ± 0.053

2.261 ± 0.027

6.576 ± 0.038

6.025 ± 0.052

10.276 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.577 ± 0.028

4.055 ± 0.037

3.915 ± 0.03

4.001 ± 0.039

4.62 ± 0.044

6.561 ± 0.042

5.365 ± 0.038

7.06 ± 0.049

1.116 ± 0.021

2.77 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski