Lake Sarah-associated circular molecule 6

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140AQI5|A0A140AQI5_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular molecule 6 OX=1685731 PE=3 SV=1
MM1 pKa = 7.74LEE3 pKa = 4.43NDD5 pKa = 3.83EE6 pKa = 4.23QPIPSVSIEE15 pKa = 4.05KK16 pKa = 10.18PLVSRR21 pKa = 11.84GWCFTCNNYY30 pKa = 9.89SEE32 pKa = 4.81SDD34 pKa = 3.7YY35 pKa = 11.67NGFLAAEE42 pKa = 4.2SVYY45 pKa = 10.9LVIGKK50 pKa = 8.97EE51 pKa = 3.91ISSTGTPHH59 pKa = 5.89LQGYY63 pKa = 9.99IYY65 pKa = 10.53FRR67 pKa = 11.84TEE69 pKa = 3.0KK70 pKa = 10.57SLRR73 pKa = 11.84TLRR76 pKa = 11.84KK77 pKa = 9.96LSDD80 pKa = 3.33RR81 pKa = 11.84SHH83 pKa = 6.61WEE85 pKa = 3.54PAKK88 pKa = 10.75GDD90 pKa = 3.65SDD92 pKa = 5.97SNFCYY97 pKa = 10.14CSKK100 pKa = 11.01GEE102 pKa = 3.96NFIEE106 pKa = 4.8RR107 pKa = 11.84GTRR110 pKa = 11.84PATGKK115 pKa = 10.32RR116 pKa = 11.84KK117 pKa = 9.35AANGLAKK124 pKa = 10.08IKK126 pKa = 10.27EE127 pKa = 4.34NYY129 pKa = 10.16AEE131 pKa = 4.18TVEE134 pKa = 4.07LAKK137 pKa = 10.47KK138 pKa = 10.5GKK140 pKa = 9.96LDD142 pKa = 4.58EE143 pKa = 5.05IDD145 pKa = 4.18PGHH148 pKa = 7.62LLRR151 pKa = 11.84FYY153 pKa = 11.45GSIKK157 pKa = 10.5SLQKK161 pKa = 11.0DD162 pKa = 3.54NLVRR166 pKa = 11.84PSDD169 pKa = 3.95SEE171 pKa = 4.11EE172 pKa = 3.98LTGLWIYY179 pKa = 10.68GPSGCGKK186 pKa = 9.8SKK188 pKa = 10.43YY189 pKa = 10.34VRR191 pKa = 11.84DD192 pKa = 3.6TYY194 pKa = 11.56GQDD197 pKa = 3.37FYY199 pKa = 12.13YY200 pKa = 11.08KK201 pKa = 10.46LANKK205 pKa = 8.32WWDD208 pKa = 3.53GYY210 pKa = 9.75RR211 pKa = 11.84QEE213 pKa = 4.16EE214 pKa = 4.38TVLIEE219 pKa = 5.02DD220 pKa = 5.38LGTEE224 pKa = 3.96HH225 pKa = 7.71DD226 pKa = 4.21KK227 pKa = 11.41LGHH230 pKa = 6.21HH231 pKa = 6.97LKK233 pKa = 10.48LWSDD237 pKa = 4.25RR238 pKa = 11.84YY239 pKa = 10.71AFSGEE244 pKa = 4.38TKK246 pKa = 10.64GGMLSCRR253 pKa = 11.84PKK255 pKa = 10.45RR256 pKa = 11.84IVVTSQYY263 pKa = 10.62SIEE266 pKa = 4.42EE267 pKa = 3.86IFLDD271 pKa = 4.32EE272 pKa = 4.31KK273 pKa = 10.25TQEE276 pKa = 3.85ALNRR280 pKa = 11.84RR281 pKa = 11.84FTKK284 pKa = 10.51LRR286 pKa = 11.84LFNLKK291 pKa = 8.53STT293 pKa = 4.16

Molecular weight: 33.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140AQI5|A0A140AQI5_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular molecule 6 OX=1685731 PE=3 SV=1
MM1 pKa = 7.74LEE3 pKa = 4.43NDD5 pKa = 3.83EE6 pKa = 4.23QPIPSVSIEE15 pKa = 4.05KK16 pKa = 10.18PLVSRR21 pKa = 11.84GWCFTCNNYY30 pKa = 9.89SEE32 pKa = 4.81SDD34 pKa = 3.7YY35 pKa = 11.67NGFLAAEE42 pKa = 4.2SVYY45 pKa = 10.9LVIGKK50 pKa = 8.97EE51 pKa = 3.91ISSTGTPHH59 pKa = 5.89LQGYY63 pKa = 9.99IYY65 pKa = 10.53FRR67 pKa = 11.84TEE69 pKa = 3.0KK70 pKa = 10.57SLRR73 pKa = 11.84TLRR76 pKa = 11.84KK77 pKa = 9.96LSDD80 pKa = 3.33RR81 pKa = 11.84SHH83 pKa = 6.61WEE85 pKa = 3.54PAKK88 pKa = 10.75GDD90 pKa = 3.65SDD92 pKa = 5.97SNFCYY97 pKa = 10.14CSKK100 pKa = 11.01GEE102 pKa = 3.96NFIEE106 pKa = 4.8RR107 pKa = 11.84GTRR110 pKa = 11.84PATGKK115 pKa = 10.32RR116 pKa = 11.84KK117 pKa = 9.35AANGLAKK124 pKa = 10.08IKK126 pKa = 10.27EE127 pKa = 4.34NYY129 pKa = 10.16AEE131 pKa = 4.18TVEE134 pKa = 4.07LAKK137 pKa = 10.47KK138 pKa = 10.5GKK140 pKa = 9.96LDD142 pKa = 4.58EE143 pKa = 5.05IDD145 pKa = 4.18PGHH148 pKa = 7.62LLRR151 pKa = 11.84FYY153 pKa = 11.45GSIKK157 pKa = 10.5SLQKK161 pKa = 11.0DD162 pKa = 3.54NLVRR166 pKa = 11.84PSDD169 pKa = 3.95SEE171 pKa = 4.11EE172 pKa = 3.98LTGLWIYY179 pKa = 10.68GPSGCGKK186 pKa = 9.8SKK188 pKa = 10.43YY189 pKa = 10.34VRR191 pKa = 11.84DD192 pKa = 3.6TYY194 pKa = 11.56GQDD197 pKa = 3.37FYY199 pKa = 12.13YY200 pKa = 11.08KK201 pKa = 10.46LANKK205 pKa = 8.32WWDD208 pKa = 3.53GYY210 pKa = 9.75RR211 pKa = 11.84QEE213 pKa = 4.16EE214 pKa = 4.38TVLIEE219 pKa = 5.02DD220 pKa = 5.38LGTEE224 pKa = 3.96HH225 pKa = 7.71DD226 pKa = 4.21KK227 pKa = 11.41LGHH230 pKa = 6.21HH231 pKa = 6.97LKK233 pKa = 10.48LWSDD237 pKa = 4.25RR238 pKa = 11.84YY239 pKa = 10.71AFSGEE244 pKa = 4.38TKK246 pKa = 10.64GGMLSCRR253 pKa = 11.84PKK255 pKa = 10.45RR256 pKa = 11.84IVVTSQYY263 pKa = 10.62SIEE266 pKa = 4.42EE267 pKa = 3.86IFLDD271 pKa = 4.32EE272 pKa = 4.31KK273 pKa = 10.25TQEE276 pKa = 3.85ALNRR280 pKa = 11.84RR281 pKa = 11.84FTKK284 pKa = 10.51LRR286 pKa = 11.84LFNLKK291 pKa = 8.53STT293 pKa = 4.16

Molecular weight: 33.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

293

293

293

293.0

33.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.096 ± 0.0

2.048 ± 0.0

5.461 ± 0.0

8.532 ± 0.0

3.754 ± 0.0

8.191 ± 0.0

2.048 ± 0.0

4.778 ± 0.0

8.874 ± 0.0

9.898 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

0.683 ± 0.0

4.096 ± 0.0

3.413 ± 0.0

2.389 ± 0.0

6.143 ± 0.0

8.874 ± 0.0

5.802 ± 0.0

3.413 ± 0.0

2.048 ± 0.0

5.461 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski