Mycolicibacterium paratuberculosis (strain ATCC BAA-968 / K-10) (Mycobacterium paratuberculosis)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium avium complex (MAC); Mycobacterium avium; Mycobacterium avium subsp. paratuberculosis

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4316 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q73SZ7|Q73SZ7_MYCPA EchA8_2 OS=Mycolicibacterium paratuberculosis (strain ATCC BAA-968 / K-10) OX=262316 GN=echA8 PE=4 SV=1
MM1 pKa = 7.87ADD3 pKa = 4.58DD4 pKa = 5.25KK5 pKa = 11.41DD6 pKa = 3.86ASSPGTEE13 pKa = 3.6AFVPDD18 pKa = 5.61FSDD21 pKa = 5.39DD22 pKa = 4.15DD23 pKa = 4.28DD24 pKa = 4.93AADD27 pKa = 4.06TGTQSWAPDD36 pKa = 3.61FDD38 pKa = 6.76DD39 pKa = 5.82DD40 pKa = 6.3AEE42 pKa = 6.06DD43 pKa = 4.86DD44 pKa = 4.01DD45 pKa = 6.57AEE47 pKa = 4.32AAEE50 pKa = 4.36PAAEE54 pKa = 4.19RR55 pKa = 11.84EE56 pKa = 4.38PEE58 pKa = 4.23PEE60 pKa = 4.32PEE62 pKa = 4.59PEE64 pKa = 4.07PEE66 pKa = 3.95PAGFAGPVPPVAVPGRR82 pKa = 11.84YY83 pKa = 8.7FYY85 pKa = 11.14VKK87 pKa = 8.04WWKK90 pKa = 10.38LVLVLLAVWAASAVIGLGLFSWWYY114 pKa = 10.39HH115 pKa = 6.49SIDD118 pKa = 3.35KK119 pKa = 10.06TPALFAVLVFVMVCVVGGVMLAMVEE144 pKa = 3.95ARR146 pKa = 11.84PLISALAVAVLSGPFAALAAAAPLYY171 pKa = 10.41GYY173 pKa = 8.23YY174 pKa = 9.31YY175 pKa = 9.54CARR178 pKa = 11.84VGHH181 pKa = 6.26CLVGVVPYY189 pKa = 10.88

Molecular weight: 20.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P61744|GPDA2_MYCPA Glycerol-3-phosphate dehydrogenase 2 [NAD(P)+] OS=Mycolicibacterium paratuberculosis (strain ATCC BAA-968 / K-10) OX=262316 GN=gpsA2 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSGRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.66GRR42 pKa = 11.84RR43 pKa = 11.84ALSAA47 pKa = 3.69

Molecular weight: 5.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4316

0

4316

1455413

37

6384

337.2

36.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.413 ± 0.05

0.859 ± 0.01

6.129 ± 0.028

5.003 ± 0.031

2.947 ± 0.019

8.963 ± 0.031

2.311 ± 0.017

4.082 ± 0.024

2.125 ± 0.024

9.835 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.996 ± 0.015

2.19 ± 0.018

6.184 ± 0.035

3.099 ± 0.022

7.781 ± 0.043

5.246 ± 0.025

5.744 ± 0.023

8.474 ± 0.035

1.491 ± 0.017

2.129 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski