Hybrid snakehead virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; unclassified Rhabdoviridae; Siniperca chuatsi rhabdovirus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M9T5K3|M9T5K3_9RHAB GDP polyribonucleotidyltransferase OS=Hybrid snakehead virus OX=1316453 GN=L PE=4 SV=1
MM1 pKa = 7.82AKK3 pKa = 9.93PVFQISYY10 pKa = 10.12DD11 pKa = 3.89LEE13 pKa = 4.69KK14 pKa = 10.78LATNFEE20 pKa = 4.36EE21 pKa = 5.47LNLAEE26 pKa = 4.67LKK28 pKa = 10.94RR29 pKa = 11.84NGEE32 pKa = 4.29DD33 pKa = 3.66PDD35 pKa = 5.66DD36 pKa = 4.41PLVWKK41 pKa = 9.22PSPDD45 pKa = 3.69LLPYY49 pKa = 9.97AASPEE54 pKa = 4.07EE55 pKa = 3.98EE56 pKa = 4.61EE57 pKa = 4.53YY58 pKa = 11.03EE59 pKa = 4.07QGEE62 pKa = 4.26QEE64 pKa = 4.22MEE66 pKa = 4.26CTDD69 pKa = 3.93EE70 pKa = 3.99YY71 pKa = 11.37TPADD75 pKa = 3.9SSWTAEE81 pKa = 4.05VSRR84 pKa = 11.84PPILPGYY91 pKa = 5.58TTQSRR96 pKa = 11.84WFGPGVTAEE105 pKa = 4.26TKK107 pKa = 10.38PIILEE112 pKa = 3.92EE113 pKa = 4.1LASVLQQIGIKK124 pKa = 9.88LISAAPSADD133 pKa = 2.91ANYY136 pKa = 10.66YY137 pKa = 10.38LLYY140 pKa = 10.23PNIHH144 pKa = 6.53MSLMAPGVLEE154 pKa = 4.02SGRR157 pKa = 11.84PLTPYY162 pKa = 7.8TAPAEE167 pKa = 4.31APKK170 pKa = 10.36PPAAPAPDD178 pKa = 4.2PPKK181 pKa = 10.15EE182 pKa = 4.29SPPAPRR188 pKa = 11.84QPVPALAEE196 pKa = 3.88QSPTGLILNDD206 pKa = 3.65DD207 pKa = 4.24DD208 pKa = 4.13VRR210 pKa = 11.84AVLWASGVRR219 pKa = 11.84VVEE222 pKa = 4.14KK223 pKa = 9.66KK224 pKa = 8.77TNKK227 pKa = 8.67TAILKK232 pKa = 9.4PCALGWMIDD241 pKa = 3.34DD242 pKa = 4.58WLQVDD247 pKa = 4.27IGAEE251 pKa = 3.88TDD253 pKa = 3.27VAEE256 pKa = 4.56IFKK259 pKa = 11.04KK260 pKa = 10.1MLAKK264 pKa = 9.79TPSRR268 pKa = 11.84KK269 pKa = 10.17LMMYY273 pKa = 9.94KK274 pKa = 10.33YY275 pKa = 10.13IPLEE279 pKa = 4.01VVQQMVLFF287 pKa = 4.7

Molecular weight: 31.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M9T1J2|M9T1J2_9RHAB Nucleocapsid protein OS=Hybrid snakehead virus OX=1316453 GN=N PE=4 SV=1
MM1 pKa = 7.79PLFKK5 pKa = 10.62KK6 pKa = 10.49SNKK9 pKa = 9.04KK10 pKa = 10.33SAVKK14 pKa = 9.84PYY16 pKa = 9.74QAPPPYY22 pKa = 9.98SATALTPSAPMDD34 pKa = 4.57LPDD37 pKa = 3.41NDD39 pKa = 3.91YY40 pKa = 10.8RR41 pKa = 11.84IKK43 pKa = 10.45TMMVEE48 pKa = 4.65LDD50 pKa = 3.66FKK52 pKa = 9.88ITSSIEE58 pKa = 3.58LKK60 pKa = 10.17TIGKK64 pKa = 9.49IYY66 pKa = 10.49QIAQYY71 pKa = 10.16MLDD74 pKa = 4.28EE75 pKa = 4.24YY76 pKa = 10.57TGPIRR81 pKa = 11.84SKK83 pKa = 9.84PLYY86 pKa = 9.08MGLFLASCHH95 pKa = 5.37NAINPSMVHH104 pKa = 5.99GKK106 pKa = 6.85WHH108 pKa = 6.18YY109 pKa = 9.47EE110 pKa = 3.52IQFRR114 pKa = 11.84GPIGFNLANNTPLDD128 pKa = 3.9WVCNPVAISYY138 pKa = 7.69EE139 pKa = 4.34CNTPEE144 pKa = 4.32RR145 pKa = 11.84SLVSYY150 pKa = 7.8TCNMRR155 pKa = 11.84PTKK158 pKa = 8.7MTGSSFEE165 pKa = 4.19KK166 pKa = 9.51MFHH169 pKa = 6.02GVLVHH174 pKa = 6.53PSAASVFGIFQMAEE188 pKa = 3.7AEE190 pKa = 4.35VRR192 pKa = 11.84GEE194 pKa = 4.38DD195 pKa = 3.58IVFILKK201 pKa = 10.56DD202 pKa = 3.92PGHH205 pKa = 6.18SWHH208 pKa = 6.92

Molecular weight: 23.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3510

208

2078

702.0

79.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.496 ± 1.421

1.51 ± 0.299

5.271 ± 0.359

6.581 ± 0.419

3.932 ± 0.382

5.84 ± 0.408

2.536 ± 0.496

5.954 ± 0.162

6.581 ± 0.517

10.427 ± 0.715

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.162 ± 0.322

4.501 ± 0.288

4.986 ± 1.211

3.675 ± 0.199

4.786 ± 0.541

7.607 ± 0.679

5.755 ± 0.309

5.242 ± 0.495

1.852 ± 0.12

3.305 ± 0.348
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski