Microbacterium phage WaterT

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 121 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514TXC2|A0A514TXC2_9CAUD Cas4 family exonuclease OS=Microbacterium phage WaterT OX=2593342 GN=75 PE=4 SV=1
MM1 pKa = 7.18TRR3 pKa = 11.84TVTKK7 pKa = 10.26QGHH10 pKa = 6.56ADD12 pKa = 3.28WDD14 pKa = 4.05DD15 pKa = 5.08LIGKK19 pKa = 8.54SFHH22 pKa = 6.43FLFYY26 pKa = 10.49PGWDD30 pKa = 3.57DD31 pKa = 4.84PEE33 pKa = 4.69WIPITLAGIDD43 pKa = 3.49LTEE46 pKa = 4.47NEE48 pKa = 4.88FGTPIYY54 pKa = 11.18YY55 pKa = 10.99LMDD58 pKa = 4.31EE59 pKa = 4.37NGGGYY64 pKa = 7.68MQFEE68 pKa = 4.65STVVEE73 pKa = 4.48VMLNDD78 pKa = 3.47

Molecular weight: 8.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514TX85|A0A514TX85_9CAUD Minor tail protein OS=Microbacterium phage WaterT OX=2593342 GN=39 PE=4 SV=1
MM1 pKa = 7.23KK2 pKa = 10.25FVVPFLRR9 pKa = 11.84ARR11 pKa = 11.84SSTGMVSSLRR21 pKa = 11.84LRR23 pKa = 11.84TPVAVASIVALSRR36 pKa = 11.84LTTTRR41 pKa = 11.84FRR43 pKa = 11.84ILMTSKK49 pKa = 10.81SKK51 pKa = 10.86LLTRR55 pKa = 11.84KK56 pKa = 8.23RR57 pKa = 11.84TKK59 pKa = 10.83DD60 pKa = 3.25GTT62 pKa = 3.74

Molecular weight: 6.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

121

0

121

18373

30

1085

151.8

16.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.77 ± 0.544

0.544 ± 0.1

6.754 ± 0.262

7.092 ± 0.333

3.576 ± 0.128

8.502 ± 0.315

1.502 ± 0.144

4.991 ± 0.158

4.185 ± 0.239

7.522 ± 0.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.368 ± 0.136

3.826 ± 0.209

4.392 ± 0.294

3.222 ± 0.243

5.813 ± 0.237

6.352 ± 0.251

6.733 ± 0.316

7.19 ± 0.237

2.134 ± 0.149

3.532 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski