Amino acid dipepetide frequency for Natronomonas sp. CBA1133

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.344AlaAla: 13.344 ± 0.188
0.802AlaCys: 0.802 ± 0.037
9.626AlaAsp: 9.626 ± 0.142
8.061AlaGlu: 8.061 ± 0.133
3.974AlaPhe: 3.974 ± 0.073
10.285AlaGly: 10.285 ± 0.163
1.867AlaHis: 1.867 ± 0.054
4.43AlaIle: 4.43 ± 0.091
1.903AlaLys: 1.903 ± 0.056
10.545AlaLeu: 10.545 ± 0.171
2.085AlaMet: 2.085 ± 0.049
2.586AlaAsn: 2.586 ± 0.055
3.938AlaPro: 3.938 ± 0.09
2.44AlaGln: 2.44 ± 0.053
7.011AlaArg: 7.011 ± 0.109
5.662AlaSer: 5.662 ± 0.086
8.022AlaThr: 8.022 ± 0.126
10.979AlaVal: 10.979 ± 0.172
1.178AlaTrp: 1.178 ± 0.043
2.736AlaTyr: 2.736 ± 0.067
0.0AlaXaa: 0.0 ± 0.0
Cys
0.632CysAla: 0.632 ± 0.032
0.088CysCys: 0.088 ± 0.01
0.58CysAsp: 0.58 ± 0.031
0.578CysGlu: 0.578 ± 0.027
0.199CysPhe: 0.199 ± 0.017
0.919CysGly: 0.919 ± 0.038
0.174CysHis: 0.174 ± 0.017
0.253CysIle: 0.253 ± 0.019
0.107CysLys: 0.107 ± 0.012
0.621CysLeu: 0.621 ± 0.028
0.102CysMet: 0.102 ± 0.012
0.181CysAsn: 0.181 ± 0.017
0.543CysPro: 0.543 ± 0.027
0.168CysGln: 0.168 ± 0.016
0.54CysArg: 0.54 ± 0.03
0.448CysSer: 0.448 ± 0.028
0.404CysThr: 0.404 ± 0.028
0.511CysVal: 0.511 ± 0.023
0.099CysTrp: 0.099 ± 0.012
0.188CysTyr: 0.188 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
10.499AspAla: 10.499 ± 0.15
0.745AspCys: 0.745 ± 0.039
7.459AspAsp: 7.459 ± 0.133
7.756AspGlu: 7.756 ± 0.128
1.972AspPhe: 1.972 ± 0.056
8.059AspGly: 8.059 ± 0.134
1.931AspHis: 1.931 ± 0.052
4.048AspIle: 4.048 ± 0.078
0.983AspLys: 0.983 ± 0.04
6.45AspLeu: 6.45 ± 0.107
1.116AspMet: 1.116 ± 0.037
1.365AspAsn: 1.365 ± 0.051
4.293AspPro: 4.293 ± 0.084
1.601AspGln: 1.601 ± 0.055
6.212AspArg: 6.212 ± 0.097
4.133AspSer: 4.133 ± 0.082
4.863AspThr: 4.863 ± 0.092
6.726AspVal: 6.726 ± 0.098
0.92AspTrp: 0.92 ± 0.039
1.815AspTyr: 1.815 ± 0.052
0.0AspXaa: 0.0 ± 0.0
Glu
8.339GluAla: 8.339 ± 0.121
0.548GluCys: 0.548 ± 0.032
4.849GluAsp: 4.849 ± 0.102
6.717GluGlu: 6.717 ± 0.144
2.955GluPhe: 2.955 ± 0.067
5.185GluGly: 5.185 ± 0.08
1.897GluHis: 1.897 ± 0.054
3.329GluIle: 3.329 ± 0.077
1.594GluLys: 1.594 ± 0.058
7.518GluLeu: 7.518 ± 0.12
2.022GluMet: 2.022 ± 0.051
2.117GluAsn: 2.117 ± 0.054
4.103GluPro: 4.103 ± 0.082
3.452GluGln: 3.452 ± 0.086
7.345GluArg: 7.345 ± 0.116
5.431GluSer: 5.431 ± 0.096
7.413GluThr: 7.413 ± 0.114
5.515GluVal: 5.515 ± 0.101
1.165GluTrp: 1.165 ± 0.042
2.817GluTyr: 2.817 ± 0.063
0.0GluXaa: 0.0 ± 0.0
Phe
3.807PheAla: 3.807 ± 0.088
0.287PheCys: 0.287 ± 0.021
3.374PheAsp: 3.374 ± 0.071
3.16PheGlu: 3.16 ± 0.064
0.97PhePhe: 0.97 ± 0.045
3.346PheGly: 3.346 ± 0.085
0.604PheHis: 0.604 ± 0.032
1.23PheIle: 1.23 ± 0.051
0.466PheLys: 0.466 ± 0.028
2.695PheLeu: 2.695 ± 0.083
0.496PheMet: 0.496 ± 0.03
0.637PheAsn: 0.637 ± 0.035
1.345PhePro: 1.345 ± 0.04
0.806PheGln: 0.806 ± 0.038
1.815PheArg: 1.815 ± 0.051
1.698PheSer: 1.698 ± 0.049
2.113PheThr: 2.113 ± 0.06
3.008PheVal: 3.008 ± 0.071
0.324PheTrp: 0.324 ± 0.021
0.83PheTyr: 0.83 ± 0.034
0.0PheXaa: 0.0 ± 0.0
Gly
7.746GlyAla: 7.746 ± 0.135
0.676GlyCys: 0.676 ± 0.031
6.691GlyAsp: 6.691 ± 0.108
7.54GlyGlu: 7.54 ± 0.116
3.204GlyPhe: 3.204 ± 0.074
7.243GlyGly: 7.243 ± 0.116
1.716GlyHis: 1.716 ± 0.056
4.038GlyIle: 4.038 ± 0.086
1.878GlyLys: 1.878 ± 0.049
7.589GlyLeu: 7.589 ± 0.143
1.631GlyMet: 1.631 ± 0.047
1.977GlyAsn: 1.977 ± 0.064
3.263GlyPro: 3.263 ± 0.065
2.078GlyGln: 2.078 ± 0.061
4.778GlyArg: 4.778 ± 0.085
4.834GlySer: 4.834 ± 0.091
5.63GlyThr: 5.63 ± 0.096
7.927GlyVal: 7.927 ± 0.136
1.027GlyTrp: 1.027 ± 0.042
2.856GlyTyr: 2.856 ± 0.07
0.0GlyXaa: 0.0 ± 0.0
His
2.034HisAla: 2.034 ± 0.05
0.173HisCys: 0.173 ± 0.015
2.056HisAsp: 2.056 ± 0.05
1.854HisGlu: 1.854 ± 0.054
0.508HisPhe: 0.508 ± 0.025
1.918HisGly: 1.918 ± 0.052
0.532HisHis: 0.532 ± 0.029
0.834HisIle: 0.834 ± 0.034
0.315HisLys: 0.315 ± 0.023
1.679HisLeu: 1.679 ± 0.044
0.277HisMet: 0.277 ± 0.019
0.445HisAsn: 0.445 ± 0.024
1.143HisPro: 1.143 ± 0.042
0.472HisGln: 0.472 ± 0.026
1.257HisArg: 1.257 ± 0.046
0.824HisSer: 0.824 ± 0.036
1.175HisThr: 1.175 ± 0.045
1.779HisVal: 1.779 ± 0.055
0.192HisTrp: 0.192 ± 0.018
0.564HisTyr: 0.564 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
4.682IleAla: 4.682 ± 0.093
0.242IleCys: 0.242 ± 0.019
3.902IleAsp: 3.902 ± 0.076
4.319IleGlu: 4.319 ± 0.091
0.899IlePhe: 0.899 ± 0.038
3.361IleGly: 3.361 ± 0.076
0.864IleHis: 0.864 ± 0.037
1.466IleIle: 1.466 ± 0.053
0.67IleLys: 0.67 ± 0.034
2.937IleLeu: 2.937 ± 0.084
0.458IleMet: 0.458 ± 0.026
0.917IleAsn: 0.917 ± 0.035
2.1IlePro: 2.1 ± 0.057
1.118IleGln: 1.118 ± 0.041
2.621IleArg: 2.621 ± 0.061
2.01IleSer: 2.01 ± 0.058
2.351IleThr: 2.351 ± 0.066
3.37IleVal: 3.37 ± 0.078
0.278IleTrp: 0.278 ± 0.02
0.842IleTyr: 0.842 ± 0.039
0.0IleXaa: 0.0 ± 0.0
Lys
1.645LysAla: 1.645 ± 0.051
0.135LysCys: 0.135 ± 0.013
0.94LysAsp: 0.94 ± 0.045
1.318LysGlu: 1.318 ± 0.043
0.512LysPhe: 0.512 ± 0.027
1.267LysGly: 1.267 ± 0.042
0.427LysHis: 0.427 ± 0.024
0.682LysIle: 0.682 ± 0.036
0.553LysLys: 0.553 ± 0.034
1.791LysLeu: 1.791 ± 0.058
0.354LysMet: 0.354 ± 0.022
0.544LysAsn: 0.544 ± 0.029
0.905LysPro: 0.905 ± 0.037
0.884LysGln: 0.884 ± 0.033
1.615LysArg: 1.615 ± 0.054
1.119LysSer: 1.119 ± 0.043
1.352LysThr: 1.352 ± 0.047
1.093LysVal: 1.093 ± 0.046
0.228LysTrp: 0.228 ± 0.017
0.526LysTyr: 0.526 ± 0.027
0.0LysXaa: 0.0 ± 0.0
Leu
12.53LeuAla: 12.53 ± 0.206
0.567LeuCys: 0.567 ± 0.032
8.326LeuAsp: 8.326 ± 0.115
5.95LeuGlu: 5.95 ± 0.115
3.108LeuPhe: 3.108 ± 0.089
7.451LeuGly: 7.451 ± 0.134
1.571LeuHis: 1.571 ± 0.05
2.713LeuIle: 2.713 ± 0.076
1.5LeuLys: 1.5 ± 0.052
8.751LeuLeu: 8.751 ± 0.162
1.315LeuMet: 1.315 ± 0.045
1.833LeuAsn: 1.833 ± 0.056
4.22LeuPro: 4.22 ± 0.077
2.092LeuGln: 2.092 ± 0.055
5.644LeuArg: 5.644 ± 0.092
5.541LeuSer: 5.541 ± 0.09
5.721LeuThr: 5.721 ± 0.101
8.477LeuVal: 8.477 ± 0.141
0.834LeuTrp: 0.834 ± 0.038
2.138LeuTyr: 2.138 ± 0.054
0.0LeuXaa: 0.0 ± 0.0
Met
1.796MetAla: 1.796 ± 0.047
0.136MetCys: 0.136 ± 0.013
1.326MetAsp: 1.326 ± 0.048
1.157MetGlu: 1.157 ± 0.041
0.523MetPhe: 0.523 ± 0.026
1.396MetGly: 1.396 ± 0.042
0.373MetHis: 0.373 ± 0.025
0.564MetIle: 0.564 ± 0.028
0.393MetLys: 0.393 ± 0.023
1.482MetLeu: 1.482 ± 0.047
0.256MetMet: 0.256 ± 0.019
0.608MetAsn: 0.608 ± 0.029
0.774MetPro: 0.774 ± 0.032
0.533MetGln: 0.533 ± 0.03
1.09MetArg: 1.09 ± 0.036
1.599MetSer: 1.599 ± 0.045
1.434MetThr: 1.434 ± 0.045
1.172MetVal: 1.172 ± 0.04
0.138MetTrp: 0.138 ± 0.013
0.376MetTyr: 0.376 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
2.614AsnAla: 2.614 ± 0.065
0.221AsnCys: 0.221 ± 0.018
1.691AsnAsp: 1.691 ± 0.051
1.832AsnGlu: 1.832 ± 0.056
0.649AsnPhe: 0.649 ± 0.031
1.917AsnGly: 1.917 ± 0.058
0.48AsnHis: 0.48 ± 0.025
1.024AsnIle: 1.024 ± 0.04
0.483AsnLys: 0.483 ± 0.029
1.983AsnLeu: 1.983 ± 0.052
0.448AsnMet: 0.448 ± 0.025
0.543AsnAsn: 0.543 ± 0.027
1.562AsnPro: 1.562 ± 0.042
0.643AsnGln: 0.643 ± 0.034
1.569AsnArg: 1.569 ± 0.052
1.038AsnSer: 1.038 ± 0.039
1.389AsnThr: 1.389 ± 0.049
2.141AsnVal: 2.141 ± 0.059
0.252AsnTrp: 0.252 ± 0.02
0.608AsnTyr: 0.608 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
4.748ProAla: 4.748 ± 0.084
0.227ProCys: 0.227 ± 0.017
4.916ProAsp: 4.916 ± 0.085
4.574ProGlu: 4.574 ± 0.085
1.608ProPhe: 1.608 ± 0.044
3.613ProGly: 3.613 ± 0.064
0.906ProHis: 0.906 ± 0.037
1.722ProIle: 1.722 ± 0.05
0.827ProLys: 0.827 ± 0.032
3.954ProLeu: 3.954 ± 0.08
0.81ProMet: 0.81 ± 0.03
1.115ProAsn: 1.115 ± 0.043
2.106ProPro: 2.106 ± 0.062
1.061ProGln: 1.061 ± 0.035
2.299ProArg: 2.299 ± 0.056
2.622ProSer: 2.622 ± 0.066
3.7ProThr: 3.7 ± 0.09
4.181ProVal: 4.181 ± 0.075
0.441ProTrp: 0.441 ± 0.026
1.119ProTyr: 1.119 ± 0.042
0.0ProXaa: 0.0 ± 0.0
Gln
2.632GlnAla: 2.632 ± 0.069
0.187GlnCys: 0.187 ± 0.016
1.23GlnAsp: 1.23 ± 0.045
1.887GlnGlu: 1.887 ± 0.053
1.275GlnPhe: 1.275 ± 0.048
1.69GlnGly: 1.69 ± 0.046
0.537GlnHis: 0.537 ± 0.026
1.134GlnIle: 1.134 ± 0.045
0.607GlnLys: 0.607 ± 0.034
2.682GlnLeu: 2.682 ± 0.062
0.601GlnMet: 0.601 ± 0.032
0.811GlnAsn: 0.811 ± 0.038
1.336GlnPro: 1.336 ± 0.039
1.217GlnGln: 1.217 ± 0.052
2.099GlnArg: 2.099 ± 0.06
1.694GlnSer: 1.694 ± 0.051
2.007GlnThr: 2.007 ± 0.059
1.894GlnVal: 1.894 ± 0.056
0.33GlnTrp: 0.33 ± 0.025
0.92GlnTyr: 0.92 ± 0.037
0.0GlnXaa: 0.0 ± 0.0
Arg
6.893ArgAla: 6.893 ± 0.112
0.475ArgCys: 0.475 ± 0.028
5.33ArgAsp: 5.33 ± 0.089
7.022ArgGlu: 7.022 ± 0.117
2.394ArgPhe: 2.394 ± 0.058
4.394ArgGly: 4.394 ± 0.081
1.237ArgHis: 1.237 ± 0.039
2.798ArgIle: 2.798 ± 0.061
1.299ArgLys: 1.299 ± 0.043
6.652ArgLeu: 6.652 ± 0.098
1.255ArgMet: 1.255 ± 0.04
1.48ArgAsn: 1.48 ± 0.047
2.528ArgPro: 2.528 ± 0.06
1.84ArgGln: 1.84 ± 0.054
4.879ArgArg: 4.879 ± 0.113
3.314ArgSer: 3.314 ± 0.068
3.85ArgThr: 3.85 ± 0.08
5.711ArgVal: 5.711 ± 0.091
0.752ArgTrp: 0.752 ± 0.035
1.972ArgTyr: 1.972 ± 0.049
0.0ArgXaa: 0.0 ± 0.0
Ser
5.014SerAla: 5.014 ± 0.083
0.323SerCys: 0.323 ± 0.021
4.467SerAsp: 4.467 ± 0.09
4.393SerGlu: 4.393 ± 0.085
1.949SerPhe: 1.949 ± 0.056
5.153SerGly: 5.153 ± 0.097
1.088SerHis: 1.088 ± 0.039
2.315SerIle: 2.315 ± 0.058
1.207SerLys: 1.207 ± 0.041
5.054SerLeu: 5.054 ± 0.081
1.072SerMet: 1.072 ± 0.042
1.357SerAsn: 1.357 ± 0.049
2.677SerPro: 2.677 ± 0.068
1.477SerGln: 1.477 ± 0.045
3.329SerArg: 3.329 ± 0.076
2.883SerSer: 2.883 ± 0.083
3.407SerThr: 3.407 ± 0.072
5.093SerVal: 5.093 ± 0.097
0.578SerTrp: 0.578 ± 0.033
1.577SerTyr: 1.577 ± 0.044
0.0SerXaa: 0.0 ± 0.0
Thr
7.615ThrAla: 7.615 ± 0.123
0.363ThrCys: 0.363 ± 0.021
6.222ThrAsp: 6.222 ± 0.105
5.17ThrGlu: 5.17 ± 0.09
2.178ThrPhe: 2.178 ± 0.059
5.945ThrGly: 5.945 ± 0.096
1.286ThrHis: 1.286 ± 0.037
2.728ThrIle: 2.728 ± 0.062
1.022ThrLys: 1.022 ± 0.034
6.631ThrLeu: 6.631 ± 0.105
0.992ThrMet: 0.992 ± 0.039
1.534ThrAsn: 1.534 ± 0.053
3.857ThrPro: 3.857 ± 0.1
1.702ThrGln: 1.702 ± 0.051
3.773ThrArg: 3.773 ± 0.082
2.883ThrSer: 2.883 ± 0.064
4.842ThrThr: 4.842 ± 0.112
7.412ThrVal: 7.412 ± 0.124
0.617ThrTrp: 0.617 ± 0.031
1.752ThrTyr: 1.752 ± 0.052
0.0ThrXaa: 0.0 ± 0.0
Val
11.124ValAla: 11.124 ± 0.17
0.788ValCys: 0.788 ± 0.032
6.561ValAsp: 6.561 ± 0.1
7.686ValGlu: 7.686 ± 0.119
2.951ValPhe: 2.951 ± 0.069
8.098ValGly: 8.098 ± 0.158
1.704ValHis: 1.704 ± 0.04
3.011ValIle: 3.011 ± 0.078
1.389ValLys: 1.389 ± 0.045
7.543ValLeu: 7.543 ± 0.112
1.257ValMet: 1.257 ± 0.043
2.089ValAsn: 2.089 ± 0.059
4.148ValPro: 4.148 ± 0.077
1.97ValGln: 1.97 ± 0.054
5.409ValArg: 5.409 ± 0.083
4.936ValSer: 4.936 ± 0.085
6.545ValThr: 6.545 ± 0.099
8.967ValVal: 8.967 ± 0.156
0.754ValTrp: 0.754 ± 0.035
2.052ValTyr: 2.052 ± 0.055
0.0ValXaa: 0.0 ± 0.0
Trp
0.908TrpAla: 0.908 ± 0.04
0.103TrpCys: 0.103 ± 0.013
0.665TrpAsp: 0.665 ± 0.03
0.856TrpGlu: 0.856 ± 0.038
0.402TrpPhe: 0.402 ± 0.029
0.754TrpGly: 0.754 ± 0.035
0.239TrpHis: 0.239 ± 0.02
0.34TrpIle: 0.34 ± 0.024
0.263TrpLys: 0.263 ± 0.018
1.2TrpLeu: 1.2 ± 0.043
0.214TrpMet: 0.214 ± 0.018
0.322TrpAsn: 0.322 ± 0.023
0.438TrpPro: 0.438 ± 0.026
0.412TrpGln: 0.412 ± 0.024
0.862TrpArg: 0.862 ± 0.037
0.54TrpSer: 0.54 ± 0.032
0.718TrpThr: 0.718 ± 0.03
0.814TrpVal: 0.814 ± 0.039
0.195TrpTrp: 0.195 ± 0.017
0.359TrpTyr: 0.359 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.873TyrAla: 2.873 ± 0.061
0.249TyrCys: 0.249 ± 0.019
2.732TyrAsp: 2.732 ± 0.057
2.485TyrGlu: 2.485 ± 0.066
0.779TyrPhe: 0.779 ± 0.034
2.329TyrGly: 2.329 ± 0.057
0.593TyrHis: 0.593 ± 0.027
0.749TyrIle: 0.749 ± 0.032
0.433TyrLys: 0.433 ± 0.024
2.526TyrLeu: 2.526 ± 0.065
0.362TyrMet: 0.362 ± 0.024
0.625TyrAsn: 0.625 ± 0.033
1.272TyrPro: 1.272 ± 0.038
0.807TyrGln: 0.807 ± 0.034
1.928TyrArg: 1.928 ± 0.061
1.201TyrSer: 1.201 ± 0.044
1.59TyrThr: 1.59 ± 0.049
2.212TyrVal: 2.212 ± 0.06
0.303TyrTrp: 0.303 ± 0.022
0.814TyrTyr: 0.814 ± 0.037
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2582 proteins (718440 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski