bacterium 1XD42-8

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A9FS86|A0A3A9FS86_9BACT Metallopeptidase OS=bacterium 1XD42-8 OX=2320102 GN=D7X25_15315 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 8.98KK3 pKa = 8.2TLASILAAFLSLSLVACGGGNSGNSAASGSNDD35 pKa = 3.29QGGGAASSAAGQASGEE51 pKa = 4.37STGKK55 pKa = 10.33KK56 pKa = 9.91VGIAMPTQDD65 pKa = 3.76LEE67 pKa = 3.77RR68 pKa = 11.84WNRR71 pKa = 11.84DD72 pKa = 2.54GAYY75 pKa = 10.41LDD77 pKa = 4.09EE78 pKa = 4.21QFKK81 pKa = 10.5AAGYY85 pKa = 6.46EE86 pKa = 4.25TVVTYY91 pKa = 11.22SNNKK95 pKa = 9.72AEE97 pKa = 4.08QQVNDD102 pKa = 4.09IQNMLAQGVDD112 pKa = 3.33ILVVAAIDD120 pKa = 4.02GEE122 pKa = 4.26ALNTVMNDD130 pKa = 2.91AGAEE134 pKa = 4.32GIPVIAYY141 pKa = 8.61DD142 pKa = 4.24RR143 pKa = 11.84LIMNDD148 pKa = 2.92NVSYY152 pKa = 10.79YY153 pKa = 11.19VSFDD157 pKa = 3.21NYY159 pKa = 10.76TVGTLQGQYY168 pKa = 10.65VINALDD174 pKa = 4.08LNNAAGPFNIEE185 pKa = 3.85FTAGDD190 pKa = 3.87PADD193 pKa = 3.87NNAGYY198 pKa = 9.9FFNGAMDD205 pKa = 3.89TLKK208 pKa = 10.8PFIDD212 pKa = 3.64SGKK215 pKa = 9.68LKK217 pKa = 10.54VVSGQTDD224 pKa = 4.33FNTVATEE231 pKa = 3.73QWQTDD236 pKa = 3.63VALDD240 pKa = 3.64RR241 pKa = 11.84AQNVLASYY249 pKa = 10.23YY250 pKa = 11.11ADD252 pKa = 3.51GTKK255 pKa = 10.4LDD257 pKa = 3.56VWVCSNDD264 pKa = 3.14STALGVAQAITSDD277 pKa = 3.85YY278 pKa = 11.46AGGNSVIMTGQDD290 pKa = 2.72GDD292 pKa = 3.9VANLRR297 pKa = 11.84NIKK300 pKa = 10.61DD301 pKa = 3.95GVQSMTVYY309 pKa = 10.58KK310 pKa = 10.62AVANEE315 pKa = 3.97AVVTLDD321 pKa = 3.57LVKK324 pKa = 10.82AILDD328 pKa = 3.69GSTVDD333 pKa = 3.48NSLITGSNWGFEE345 pKa = 4.39CAYY348 pKa = 9.07DD349 pKa = 3.56TSSYY353 pKa = 8.79FTSEE357 pKa = 3.64GHH359 pKa = 5.84NCPSFLLVPDD369 pKa = 5.01VITADD374 pKa = 3.36NMEE377 pKa = 4.69EE378 pKa = 4.19KK379 pKa = 10.55LVTPGYY385 pKa = 9.92YY386 pKa = 9.78KK387 pKa = 10.79VGADD391 pKa = 3.95GYY393 pKa = 11.58LEE395 pKa = 4.22ATNGG399 pKa = 3.41

Molecular weight: 41.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A9FPV7|A0A3A9FPV7_9BACT ABC transporter ATP-binding protein OS=bacterium 1XD42-8 OX=2320102 GN=D7X25_20675 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 8.55MTYY5 pKa = 8.37QPKK8 pKa = 8.44RR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 8.51RR12 pKa = 11.84AKK14 pKa = 8.81VHH16 pKa = 5.44GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 9.99GGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7100

0

7100

1928657

17

2390

271.6

30.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.245 ± 0.036

1.515 ± 0.015

5.104 ± 0.022

7.729 ± 0.032

4.354 ± 0.026

7.143 ± 0.031

1.805 ± 0.011

7.485 ± 0.033

7.019 ± 0.034

9.338 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.004 ± 0.015

4.216 ± 0.024

3.606 ± 0.026

3.613 ± 0.02

4.579 ± 0.023

5.952 ± 0.023

5.036 ± 0.022

6.262 ± 0.021

0.958 ± 0.01

4.039 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski