Momordica charantia associated gemycircularvirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus mocha1

Average proteome isoelectric point is 7.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A345S7U9|A0A345S7U9_9VIRU Replication-associated protein OS=Momordica charantia associated gemycircularvirus OX=2291613 PE=3 SV=1
MM1 pKa = 7.84PSFVCNFRR9 pKa = 11.84YY10 pKa = 10.28GLITYY15 pKa = 7.3PQCDD19 pKa = 3.52GLDD22 pKa = 3.43PFRR25 pKa = 11.84VMEE28 pKa = 4.46RR29 pKa = 11.84FSSLGAEE36 pKa = 4.35CIIGRR41 pKa = 11.84EE42 pKa = 3.83RR43 pKa = 11.84HH44 pKa = 5.72EE45 pKa = 5.01DD46 pKa = 3.46GGLHH50 pKa = 5.74LHH52 pKa = 6.24VFADD56 pKa = 4.7FGRR59 pKa = 11.84KK60 pKa = 8.14FRR62 pKa = 11.84SRR64 pKa = 11.84KK65 pKa = 7.79TDD67 pKa = 2.98VFDD70 pKa = 3.81VDD72 pKa = 3.65GRR74 pKa = 11.84HH75 pKa = 6.28PNVEE79 pKa = 4.04PSKK82 pKa = 9.22GTPEE86 pKa = 3.79KK87 pKa = 10.85GYY89 pKa = 10.88DD90 pKa = 3.47YY91 pKa = 10.69AIKK94 pKa = 10.83DD95 pKa = 3.47GDD97 pKa = 4.33VVCGGLGRR105 pKa = 11.84PEE107 pKa = 3.97PSGGGDD113 pKa = 3.43GASSAKK119 pKa = 8.61WARR122 pKa = 11.84ITQAEE127 pKa = 4.11NRR129 pKa = 11.84DD130 pKa = 3.9EE131 pKa = 4.33FWEE134 pKa = 4.38LVHH137 pKa = 7.01EE138 pKa = 5.14LDD140 pKa = 4.73PKK142 pKa = 10.9AAACSFNALSKK153 pKa = 9.33YY154 pKa = 10.43ADD156 pKa = 3.0WRR158 pKa = 11.84FAEE161 pKa = 4.33VPPEE165 pKa = 3.98YY166 pKa = 10.36EE167 pKa = 3.34HH168 pKa = 7.5DD169 pKa = 3.61GRR171 pKa = 11.84IEE173 pKa = 4.01FVPGDD178 pKa = 3.59ADD180 pKa = 4.72GRR182 pKa = 11.84ADD184 pKa = 3.28WVSQSGIRR192 pKa = 11.84LAEE195 pKa = 4.12PFLAGTRR202 pKa = 11.84VKK204 pKa = 10.87SLVLWGKK211 pKa = 10.19SRR213 pKa = 11.84TGKK216 pKa = 6.05TTWARR221 pKa = 11.84SLGPHH226 pKa = 6.31VYY228 pKa = 10.18CVGLVSGTEE237 pKa = 4.02CAKK240 pKa = 10.98GLDD243 pKa = 3.16ARR245 pKa = 11.84YY246 pKa = 9.64AVFDD250 pKa = 5.19DD251 pKa = 3.36IRR253 pKa = 11.84GGLGFFHH260 pKa = 7.61GYY262 pKa = 9.93KK263 pKa = 9.57EE264 pKa = 3.99WLGAQPHH271 pKa = 5.37VPIKK275 pKa = 10.13EE276 pKa = 4.14LYY278 pKa = 9.69RR279 pKa = 11.84EE280 pKa = 4.04PRR282 pKa = 11.84YY283 pKa = 9.43MRR285 pKa = 11.84WNKK288 pKa = 9.74PSIWVCNRR296 pKa = 11.84DD297 pKa = 3.62PRR299 pKa = 11.84LEE301 pKa = 4.31AYY303 pKa = 8.55PPNATPDD310 pKa = 3.91WEE312 pKa = 4.27WMDD315 pKa = 4.74DD316 pKa = 3.47NVDD319 pKa = 4.4FIEE322 pKa = 4.46VTDD325 pKa = 4.0SLIASISHH333 pKa = 7.17ASTEE337 pKa = 4.06

Molecular weight:
37.86 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A345S7U9|A0A345S7U9_9VIRU Replication-associated protein OS=Momordica charantia associated gemycircularvirus OX=2291613 PE=3 SV=1
MM1 pKa = 7.95AYY3 pKa = 9.64RR4 pKa = 11.84RR5 pKa = 11.84SVRR8 pKa = 11.84RR9 pKa = 11.84APARR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84PTRR18 pKa = 11.84KK19 pKa = 8.77SSYY22 pKa = 7.52GSRR25 pKa = 11.84YY26 pKa = 8.58RR27 pKa = 11.84RR28 pKa = 11.84SYY30 pKa = 7.13TAKK33 pKa = 10.15RR34 pKa = 11.84RR35 pKa = 11.84FTRR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 8.93RR41 pKa = 11.84PMSRR45 pKa = 11.84KK46 pKa = 9.14SLLNVTSRR54 pKa = 11.84KK55 pKa = 9.88KK56 pKa = 9.81QDD58 pKa = 2.94TMLGYY63 pKa = 11.12SNLANDD69 pKa = 4.13STDD72 pKa = 2.91GSNKK76 pKa = 8.33FHH78 pKa = 7.02SGAGILKK85 pKa = 9.23GGRR88 pKa = 11.84MYY90 pKa = 10.49IIPWIATARR99 pKa = 11.84DD100 pKa = 3.65LSPTDD105 pKa = 3.44GTLGTVAQKK114 pKa = 10.95ASRR117 pKa = 11.84TSTEE121 pKa = 3.71CYY123 pKa = 9.18MRR125 pKa = 11.84GLRR128 pKa = 11.84EE129 pKa = 4.33RR130 pKa = 11.84IQIQTNNGTSWQWRR144 pKa = 11.84RR145 pKa = 11.84ICFTFKK151 pKa = 11.06GPLLVNATTGSEE163 pKa = 3.99TQRR166 pKa = 11.84YY167 pKa = 8.48QIEE170 pKa = 4.48TNQGFARR177 pKa = 11.84VMSDD181 pKa = 3.61FALGTAADD189 pKa = 4.26PLNGVLFKK197 pKa = 11.03GLQSVDD203 pKa = 2.37WSSYY207 pKa = 7.47FTAKK211 pKa = 9.67TDD213 pKa = 3.38NSLVNIMYY221 pKa = 9.37DD222 pKa = 3.01QTRR225 pKa = 11.84IISSGNASGVMRR237 pKa = 11.84NFKK240 pKa = 9.79LWHH243 pKa = 6.22PMNKK247 pKa = 9.33NLYY250 pKa = 8.36YY251 pKa = 10.85NEE253 pKa = 4.64DD254 pKa = 3.89EE255 pKa = 4.58IGATTGTSVYY265 pKa = 9.16STSSKK270 pKa = 10.77RR271 pKa = 11.84GMGDD275 pKa = 3.32YY276 pKa = 10.78YY277 pKa = 11.59VVDD280 pKa = 5.52FILAGTGSNINDD292 pKa = 3.41LMSFEE297 pKa = 5.22PDD299 pKa = 2.85STLYY303 pKa = 8.55WHH305 pKa = 6.72EE306 pKa = 4.03RR307 pKa = 3.41

Molecular weight:
34.85 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

644

307

337

322.0

36.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.988 ± 0.566

1.553 ± 0.639

6.522 ± 1.159

4.969 ± 1.675

4.348 ± 0.542

9.161 ± 0.722

2.019 ± 0.738

4.193 ± 0.261

4.658 ± 0.161

6.366 ± 0.105

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.329 ± 0.889

4.037 ± 1.294

4.814 ± 1.334

2.174 ± 0.768

9.006 ± 1.004

8.23 ± 1.555

6.366 ± 2.183

5.28 ± 0.971

2.64 ± 0.486

4.348 ± 0.612

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski