Leptobacterium flavescens

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Leptobacterium

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3760 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P0UI54|A0A6P0UI54_9FLAO Uncharacterized protein OS=Leptobacterium flavescens OX=472055 GN=GWK08_06195 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.34KK3 pKa = 10.37LYY5 pKa = 10.52FLICYY10 pKa = 9.62LLFGAYY16 pKa = 8.52TYY18 pKa = 11.44AQITVDD24 pKa = 3.81DD25 pKa = 4.47TSFSAQQLVEE35 pKa = 4.19EE36 pKa = 4.42VLINSPCATTSNWTASTGIIPAQGVDD62 pKa = 2.97INGIGFFEE70 pKa = 5.02RR71 pKa = 11.84GTSNFPFEE79 pKa = 4.5RR80 pKa = 11.84GVVLSSGRR88 pKa = 11.84ASLAGARR95 pKa = 11.84NPVDD99 pKa = 3.91PNLNPQSEE107 pKa = 4.77GSAPTDD113 pKa = 3.11TGGAPLWRR121 pKa = 11.84GDD123 pKa = 3.55NDD125 pKa = 3.79LEE127 pKa = 4.73NIVGLPLGNTFNASSLSFDD146 pKa = 4.28FVSQANQISFNFIFASEE163 pKa = 4.52EE164 pKa = 3.85YY165 pKa = 9.85NQNFEE170 pKa = 4.7CQFSDD175 pKa = 3.04AFAFILTDD183 pKa = 3.88SSGNSQNLAVVPNTTIPITTMTIRR207 pKa = 11.84RR208 pKa = 11.84RR209 pKa = 11.84VVPTAPGSTGCPAQNPQFFEE229 pKa = 4.52RR230 pKa = 11.84YY231 pKa = 9.51NFGPTAASSAIAFNGQTVALTASGTVVPGEE261 pKa = 4.21TYY263 pKa = 9.96TIKK266 pKa = 10.81LVVADD271 pKa = 4.27QSDD274 pKa = 3.95GALDD278 pKa = 3.4SAVFLEE284 pKa = 4.64AGSFNLGGSLGDD296 pKa = 3.96DD297 pKa = 3.12RR298 pKa = 11.84TVASGNPGCDD308 pKa = 3.11GEE310 pKa = 5.98AITLDD315 pKa = 3.47ATITGATYY323 pKa = 10.41SWFILNEE330 pKa = 4.03TTNVFEE336 pKa = 5.11PLPDD340 pKa = 3.78DD341 pKa = 3.61TNPAVNEE348 pKa = 3.99TDD350 pKa = 3.25PVINVTTSGTYY361 pKa = 9.58KK362 pKa = 10.59VEE364 pKa = 4.05GEE366 pKa = 4.54LIGTCSFEE374 pKa = 4.45DD375 pKa = 3.63EE376 pKa = 4.46VVIEE380 pKa = 5.78FITPPQIEE388 pKa = 4.82NSPQDD393 pKa = 4.94LEE395 pKa = 5.11ACDD398 pKa = 4.34EE399 pKa = 4.43GNNDD403 pKa = 3.18NAIFDD408 pKa = 4.42LSVNTPVVLGSQDD421 pKa = 3.45LNQFVVSYY429 pKa = 10.76HH430 pKa = 5.78LTQADD435 pKa = 3.54ADD437 pKa = 4.42SNSGGIDD444 pKa = 3.01PAAFSNTQNPQQIFIRR460 pKa = 11.84VADD463 pKa = 3.81NTQSCFVTANFTVTAVPLSIQALDD487 pKa = 4.84DD488 pKa = 5.32LEE490 pKa = 4.82QCDD493 pKa = 4.15PDD495 pKa = 4.15GVASFDD501 pKa = 3.79LSDD504 pKa = 3.56RR505 pKa = 11.84TSDD508 pKa = 4.19VIGTNDD514 pKa = 3.53PSRR517 pKa = 11.84VTVTYY522 pKa = 10.71HH523 pKa = 6.06LTQADD528 pKa = 4.24ADD530 pKa = 4.25TANAPLPDD538 pKa = 3.62NGYY541 pKa = 11.03DD542 pKa = 3.15NTAPVQQIFVRR553 pKa = 11.84LQDD556 pKa = 3.65NANPNCFATASFNIIVNIPPVINQVDD582 pKa = 4.22DD583 pKa = 4.76IEE585 pKa = 5.07EE586 pKa = 4.42CDD588 pKa = 3.77NDD590 pKa = 5.33LDD592 pKa = 5.63GDD594 pKa = 3.98DD595 pKa = 3.79TNGFVEE601 pKa = 5.05FDD603 pKa = 3.34LSLRR607 pKa = 11.84DD608 pKa = 3.27TDD610 pKa = 5.8LINGQANVVIDD621 pKa = 4.25SYY623 pKa = 11.38HH624 pKa = 6.72SSRR627 pKa = 11.84ADD629 pKa = 3.25AEE631 pKa = 4.76SGNSPLPAIYY641 pKa = 10.35TNTTANRR648 pKa = 11.84EE649 pKa = 4.34TIFVRR654 pKa = 11.84LRR656 pKa = 11.84NTASDD661 pKa = 4.16CVSVADD667 pKa = 4.48FDD669 pKa = 5.65LVVNPLPDD677 pKa = 3.46VTNVNLLQCDD687 pKa = 3.19VDD689 pKa = 4.1GNPDD693 pKa = 2.94GFTIYY698 pKa = 10.97NLDD701 pKa = 3.75EE702 pKa = 4.67ANIDD706 pKa = 3.86IISGGNTADD715 pKa = 3.38FTFTYY720 pKa = 10.25FLSLADD726 pKa = 3.85AQSGNNPQAPAPFTNTANPQTLFVSVVNNTTSCSRR761 pKa = 11.84IVEE764 pKa = 4.5VILDD768 pKa = 3.54VTATNVSNASLTLCDD783 pKa = 4.49DD784 pKa = 4.25DD785 pKa = 6.33RR786 pKa = 11.84DD787 pKa = 3.91GSVAFDD793 pKa = 3.71LTLADD798 pKa = 3.88AQVLTSAPPGLDD810 pKa = 2.81VAYY813 pKa = 10.64YY814 pKa = 8.71ATLQDD819 pKa = 3.98AQLEE823 pKa = 4.51QNRR826 pKa = 11.84LPDD829 pKa = 4.21LYY831 pKa = 11.03SNSSNPQTVFARR843 pKa = 11.84VEE845 pKa = 3.82NANACFGISSLTLNVNEE862 pKa = 5.48LPPQNPLSDD871 pKa = 4.39FVVCDD876 pKa = 4.46DD877 pKa = 5.42DD878 pKa = 4.76DD879 pKa = 4.02TFTFDD884 pKa = 5.86LSTKK888 pKa = 9.53DD889 pKa = 3.51AEE891 pKa = 4.83VFGSANPADD900 pKa = 4.02FTVSYY905 pKa = 10.36HH906 pKa = 7.18SNDD909 pKa = 2.9ADD911 pKa = 5.0AIADD915 pKa = 4.14TNPLTTNYY923 pKa = 9.88TNISNPQTVVARR935 pKa = 11.84LEE937 pKa = 4.1NNITGCFVTTAFRR950 pKa = 11.84LSVNINPTVNQPLPLEE966 pKa = 4.4ICDD969 pKa = 4.41DD970 pKa = 4.84DD971 pKa = 4.57IVDD974 pKa = 4.37GFAAFDD980 pKa = 3.75LTTKK984 pKa = 10.33NAEE987 pKa = 3.62ITGNQNGVEE996 pKa = 3.89VSYY999 pKa = 11.49YY1000 pKa = 9.51LTQNDD1005 pKa = 3.98ADD1007 pKa = 3.87MGINPLPDD1015 pKa = 3.17TYY1017 pKa = 11.5TNISNPQIVFVRR1029 pKa = 11.84VQNTTTSCFRR1039 pKa = 11.84TDD1041 pKa = 3.02NTTLEE1046 pKa = 5.1LIVQQGAVANRR1057 pKa = 11.84PQPLQTCDD1065 pKa = 3.44EE1066 pKa = 4.56LGEE1069 pKa = 4.27NDD1071 pKa = 5.56GIAQFDD1077 pKa = 3.93LTALDD1082 pKa = 3.6NEE1084 pKa = 4.32IRR1086 pKa = 11.84GNQDD1090 pKa = 2.59PSQYY1094 pKa = 9.77RR1095 pKa = 11.84VSYY1098 pKa = 10.6HH1099 pKa = 5.14LTEE1102 pKa = 5.03SDD1104 pKa = 3.87AEE1106 pKa = 4.31AGSNEE1111 pKa = 3.83INGAYY1116 pKa = 9.45TNISNPQTIYY1126 pKa = 11.3ARR1128 pKa = 11.84VTNIPIGCFNTTTAEE1143 pKa = 4.11LVVNLLPEE1151 pKa = 4.55PEE1153 pKa = 4.95LGDD1156 pKa = 3.81TVALCVDD1163 pKa = 3.77ANGTITSRR1171 pKa = 11.84PTLNSGIPASGFSFEE1186 pKa = 4.0WRR1188 pKa = 11.84LDD1190 pKa = 3.54GTLLAGEE1197 pKa = 4.5NNPDD1201 pKa = 3.58LVANTAGTYY1210 pKa = 7.62TVSVVNNSTGCRR1222 pKa = 11.84NTDD1225 pKa = 3.07EE1226 pKa = 4.41VVVRR1230 pKa = 11.84RR1231 pKa = 11.84SSPPITFSAEE1241 pKa = 3.83VVTNAFVQNNRR1252 pKa = 11.84IEE1254 pKa = 4.3VNVTGEE1260 pKa = 3.66GDD1262 pKa = 3.76YY1263 pKa = 11.05QFSLDD1268 pKa = 3.86GGSFQDD1274 pKa = 3.74DD1275 pKa = 3.84PFFEE1279 pKa = 4.4RR1280 pKa = 11.84VSPGPHH1286 pKa = 6.95TITIRR1291 pKa = 11.84DD1292 pKa = 3.44INGCGSVEE1300 pKa = 3.8VDD1302 pKa = 3.79VFIIDD1307 pKa = 3.52YY1308 pKa = 9.85PRR1310 pKa = 11.84FFTPNGDD1317 pKa = 3.66GFNDD1321 pKa = 2.97TWNIVGATRR1330 pKa = 11.84LGNPNIFIFDD1340 pKa = 3.82RR1341 pKa = 11.84YY1342 pKa = 10.62GKK1344 pKa = 9.76LLKK1347 pKa = 10.45QIEE1350 pKa = 4.22PAGNGWNGMFNGQPLPSSDD1369 pKa = 3.01YY1370 pKa = 10.33WFLINYY1376 pKa = 8.29TEE1378 pKa = 5.32DD1379 pKa = 3.84DD1380 pKa = 3.23VSKK1383 pKa = 10.68QLRR1386 pKa = 11.84GHH1388 pKa = 6.63FALKK1392 pKa = 10.31RR1393 pKa = 3.61

Molecular weight: 149.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P0UJK0|A0A6P0UJK0_9FLAO Uncharacterized protein OS=Leptobacterium flavescens OX=472055 GN=GWK08_08510 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.13RR10 pKa = 11.84KK11 pKa = 9.48RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.49HH16 pKa = 3.94GFRR19 pKa = 11.84EE20 pKa = 4.27RR21 pKa = 11.84MASANGRR28 pKa = 11.84KK29 pKa = 9.04VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.52LSVSSEE47 pKa = 3.87PRR49 pKa = 11.84HH50 pKa = 5.92KK51 pKa = 10.61KK52 pKa = 9.84

Molecular weight: 6.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3760

0

3760

1265630

38

4047

336.6

38.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.409 ± 0.042

0.71 ± 0.014

5.749 ± 0.044

7.072 ± 0.042

5.309 ± 0.04

6.686 ± 0.038

1.783 ± 0.018

7.633 ± 0.041

7.381 ± 0.053

9.419 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.104 ± 0.023

5.694 ± 0.04

3.451 ± 0.022

3.236 ± 0.019

4.152 ± 0.027

6.558 ± 0.031

5.372 ± 0.051

6.147 ± 0.03

1.094 ± 0.017

4.041 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski