Polynucleobacter necessarius subsp. necessarius (strain STIR1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Polynucleobacter; Polynucleobacter necessarius

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1507 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B1XTZ9|B1XTZ9_POLNS Binding-protein-dependent transport systems inner membrane component OS=Polynucleobacter necessarius subsp. necessarius (strain STIR1) OX=452638 GN=Pnec_0569 PE=4 SV=1
MM1 pKa = 7.64RR2 pKa = 11.84PDD4 pKa = 3.01VDD6 pKa = 3.61PTVQQAALKK15 pKa = 10.69EE16 pKa = 4.52MFTDD20 pKa = 3.14PHH22 pKa = 6.97FNVMDD27 pKa = 4.37GLDD30 pKa = 3.88IYY32 pKa = 10.73IDD34 pKa = 4.82DD35 pKa = 4.24YY36 pKa = 11.85SKK38 pKa = 11.04PDD40 pKa = 3.52PFCYY44 pKa = 9.88QEE46 pKa = 4.67CSSEE50 pKa = 3.93

Molecular weight: 5.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|B1XSP4|RPOC_POLNS DNA-directed RNA polymerase subunit beta' OS=Polynucleobacter necessarius subsp. necessarius (strain STIR1) OX=452638 GN=rpoC PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.17RR14 pKa = 11.84THH16 pKa = 5.95GFRR19 pKa = 11.84IRR21 pKa = 11.84MKK23 pKa = 8.63TKK25 pKa = 10.02SGRR28 pKa = 11.84AVLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1507

0

1507

404725

30

1420

268.6

29.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.843 ± 0.062

1.009 ± 0.021

5.162 ± 0.049

5.815 ± 0.061

3.995 ± 0.052

7.606 ± 0.057

2.069 ± 0.035

6.387 ± 0.054

5.638 ± 0.059

10.352 ± 0.086

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.665 ± 0.033

3.841 ± 0.047

4.552 ± 0.037

4.004 ± 0.046

4.984 ± 0.053

6.217 ± 0.051

4.973 ± 0.045

6.971 ± 0.061

1.265 ± 0.029

2.65 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski